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Immunofluorescent Localization of Proteins in Schistosoma mansoni

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Schistosoma mansoni

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2151))

Abstract

Immunofluorescence allows the detection, visualization, and localization of proteins by using the ability of antibodies to firmly bind to specific antigens. Proteins must be accessible to thorough interaction with the specific antibodies. Different immune evasion mechanisms of parasites are directed to hamper or prevent access of antibodies to critical proteins or virulence factors. The blood fluke Schistosoma mansoni would not survive a day in the host blood capillaries if antibodies were able to readily bind to proteins located at the surface and mediate its attrition and demise by the complement system and/or the FcγR- or FcαR-bearing leukocytes. The worm surface is the area of parasite-host interaction and the route to critical nutrients, but is selectively permeable, allowing access of nutrient molecules but not host antibodies. Gentle procedures, which, however, are not commonly in use in vivo, are required to increase the permeability of the parasite outer membrane shield to just allow access of specific antibodies and identify and localize the proteins at the apical surface. Robust methods involving acetone, methanol, and Triton X-100 treatment lead to disintegration of the dual lipid bilayer cover with exposure of the proteins located in the tegument beneath. Internal proteins may not be accessed except following cryostat or paraffin sectioning. Accordingly, vaccine-induced specific antibodies to the apical surface or tegument proteins are unable to harm intact parasites. Specific antibodies to surface membrane proteins may only add to the action of administered or endo schistosomicides via acceleration of killing and interference with repair of severely and lightly impacted parasites, respectively. Therefore, careful immunofluorescent localization of S. mansoni proteins is important for devising the different control strategies against infection.

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Author information

Authors and Affiliations

  1. Zoology Department, Faculty of Science, Cairo University, Giza, Egypt

    Rashika El Ridi & Hatem Tallima

  2. Department of Chemistry, School of Science and Engineering, American University in Cairo, New Cairo, Egypt

    Hatem Tallima

Authors
  1. Rashika El Ridi
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  2. Hatem Tallima
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Editors and Affiliations

  1. School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK

    David J. Timson

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El Ridi, R., Tallima, H. (2020). Immunofluorescent Localization of Proteins in Schistosoma mansoni. In: Timson, D.J. (eds) Schistosoma mansoni. Methods in Molecular Biology, vol 2151. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0635-3_3

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  • DOI: https://doi.org/10.1007/978-1-0716-0635-3_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0634-6

  • Online ISBN: 978-1-0716-0635-3

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