Abstract
Computer-aided wet-spinning (CAWS) has emerged in the past few years as a hybrid fabrication technique coupling the advantages of additive manufacturing in controlling the external shape and macroporous structure of biomedical polymeric scaffold with those of wet-spinning in endowing the polymeric matrix with a spread microporosity. This book chapter is aimed at providing a detailed description of the experimental methods developed to fabricate by CAWS polymeric scaffolds with a predefined external shape and size as well as a controlled internal porous structure. The protocol for the preparation of poly(ε-caprolactone)-based scaffolds with a predefined pore size and geometry will be reported in detail as a reference example that can be followed and simply adapted to fabricate other kinds of scaffold, with a different porous structure or based on different biodegradable polymers, by applying the processing parameters reported in relevant tables included in the text.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Giannitelli SM, Mozetic P, Trombetta M, Rainer A (2015) Combined additive manufacturing approaches in tissue engineering. Acta Biomater 24:1–11
Puppi D, Zhang X, Yang L, Chiellini F, Sun X, Chiellini E (2014) Nano/microfibrous polymeric constructs loaded with bioactive agents and designed for tissue engineering applications: a review. J Biomed Mater Res B Appl Biomater 102(7):1562–1579
Puppi D, Chiellini F (2017) Wet-spinning of biomedical polymers: from single fibers production to additive manufacturing of 3D scaffolds. Polym Int 66(12):1690–1696
Puppi D, Mota C, Gazzarri M, Dinucci D, Gloria A, Myrzabekova M, Ambrosio L, Chiellini F (2012) Additive manufacturing of wet-spun polymeric scaffolds for bone tissue engineering. Biomed Microdevices 14(6):1115–1127
Puppi D, Migone C, Grassi L, Pirosa A, Maisetta G, Batoni G, Chiellini F (2016) Integrated three-dimensional fiber/hydrogel biphasic scaffolds for periodontal bone tissue engineering. Polym Int 65(6):631–640
Mota C, Puppi D, Dinucci D, Gazzarri M, Chiellini F (2013) Additive manufacturing of star poly(ε-caprolactone) wet-spun scaffolds for bone tissue engineering applications. J Bioact Compat Polym 28(4):320–340
Puppi D, Piras AM, Pirosa A, Sandreschi S, Chiellini F (2016) Levofloxacin-loaded star poly(ε-caprolactone) scaffolds by additive manufacturing. J Mater Sci Mater Med 27(3):44
Dini F, Barsotti G, Puppi D, Coli A, Briganti A, Giannessi E, Miragliotta V, Mota C, Pirosa A, Stornelli MR, Gabellieri P, Carlucci F, Chiellini F (2016) Tailored star poly (ε-caprolactone) wet-spun scaffolds for in vivo regeneration of long bone critical size defects. J Bioact Compat Polym 31(1):15–30
Neves SC, Mota C, Longoni A, Barrias CC, Granja PL, Moroni L (2016) Additive manufactured polymeric 3D scaffolds with tailored surface topography influence mesenchymal stromal cells activity. Biofabrication 8(2):025012
Mota C, Wang SY, Puppi D, Gazzarri M, Migone C, Chiellini F, Chen GQ, Chiellini E (2017) Additive manufacturing of poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] scaffolds for engineered bone development. J Tissue Eng Regen Med 11(1):175–186
Puppi D, Pirosa A, Morelli A, Chiellini F (2018) Design, fabrication and characterization of tailored poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyexanoate] scaffolds by Computer-aided Wet-spinning. Rapid Prototyp J 24(1):1–8
Puppi D, Morelli A, Chiellini F (2017) Additive Manufacturing of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/poly(ε-caprolactone) Blend Scaffolds for Tissue Engineering. Bioengineering 4(2):49
Puppi D, Migone C, Morelli A, Bartoli C, Gazzarri M, Pasini D, Chiellini F (2016) Microstructured chitosan/poly(γ-glutamic acid) polyelectrolyte complex hydrogels by computer-aided wet-spinning for biomedical three-dimensional scaffolds. J Bioact Compat Polym 31(5):531–549
Chiellini F, Puppi D, Piras AM, Morelli A, Bartoli C, Migone C (2016) Modelling of pancreatic ductal adenocarcinoma in vitro with three-dimensional microstructured hydrogels. RSC Adv 6(59):54226–54235
Puppi D, Pirosa A, Lupi G, Erba PA, Giachi G, Chiellini F (2017) Design and fabrication of novel polymeric biodegradable stents for small caliber blood vessels by computer-aided wet-spinning. Biomed Mater 12(3):035011
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Puppi, D., Chiellini, F. (2021). Computer-Aided Wet-Spinning. In: Rainer, A., Moroni, L. (eds) Computer-Aided Tissue Engineering. Methods in Molecular Biology, vol 2147. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0611-7_8
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0611-7_8
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0610-0
Online ISBN: 978-1-0716-0611-7
eBook Packages: Springer Protocols