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Tip Growth and Polarity

  • Chapter
The Growing Fungus

Abstract

Cells are said to exhibit polarized cell development or tip growth when the expansion of the cell surface is focused at one location. The term polarity has also been used in the context of mechanisms that establish new sites for growth, either by budding, germ tube formation or by branching. This chapter considers how tip growth and cell polarity are established, maintained and regulated in fungal cells.

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References

  • Adams, A.E.M. and Pringle, J.R. (1984) Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic mutant Saccharomyces cerevisiae. Journal of Cell Biology, 98, 934–45.

    Article  PubMed  CAS  Google Scholar 

  • Adams, A.E.M., Johnson, D.I., Longnecker, R.M. et al. (1990) CDC24 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae. Journal of Cell Biology, 111, 131–42.

    Article  PubMed  CAS  Google Scholar 

  • Adams, A.E.M., Botstein, D. and Drubin, D.G. (1991) Requirement of yeast fimbrin for actin organization and morphogenesis in vivo. Nature, 354, 404–8.

    Article  PubMed  CAS  Google Scholar 

  • Alfa, C.E. and Hyams, J.S. (1990) Distribution of tubulin and actin through the cell division cycle of the fission yeast Schizosaccharomyces japonicus var. versitalis: comparison with Schizosaccharomyces pombe. Journal of Cell Science, 96, 71–7.

    PubMed  Google Scholar 

  • Allen, E.D., Aiuto, R. and Sussman, A.S. (1980) Effects of cytochalasins on Neurospora crassa I. growth and ultrastructure. Protoplasma, 102, 63–75.

    Article  PubMed  CAS  Google Scholar 

  • Anderson, J. and Soil, D.R. (1986) Differences in actin localization during bud and hypha formation in the yeast Candida albicans. Journal of General Microbiology, 132, 2035–47.

    PubMed  CAS  Google Scholar 

  • Bartnicki-Garcia, S. and Bracker, C.E. (1984) Unique properties of chitosomes, in Microbial Cell Wall Synthesis and Autolysis, (ed. C. Nombela) Elsevier Science Publishers, Amsterdam, pp. 101–12.

    Google Scholar 

  • Bartnicki-Garcia, S. and Lippman, E. (1969) Fungal morphogenesis: cell wall construction in Mucor rouxii. Science, 165, 302–4.

    Article  PubMed  CAS  Google Scholar 

  • Bartnicki-Garcia, S., Bracker, C.E., Reyes, E. and Ruiz-Herrera, J. (1978) Isolation of chitosomes from taxonomically diverse fungi and synthesis of chitin microfibrils in vitro. Experimental Mycology, 2, 173–92.

    Article  CAS  Google Scholar 

  • Bartnicki-Garcia, S., Hergert, F. and Gierz, G. (1989) Computer simulation of fungal morphogenesis and the mathematical basis for hyphal (tip) growth. Protoplasma, 153, 46–57.

    Article  Google Scholar 

  • Barton, R. and Gull, K. (1988) Variation in the cytoplasmic microtubule organization and spindle length between the two forms of the dimorphic fungus Candida albicans. Journal of Cell Science, 91, 211–20.

    PubMed  Google Scholar 

  • Bedlack, Jr, R.S., Wei, M-D. and Loew, L.M. (1992) Localized membrane potential depolarizations and localized calcium influx during electric field-guided neurite growth. Neuron, 9, 393–403.

    Article  PubMed  CAS  Google Scholar 

  • Bender, A. and Pringle, J.R. (1989) Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the rasrelated gene RSR1 Proceedings of the National Academy of Sciences of the USA, 86, 9976–80.

    Article  CAS  Google Scholar 

  • Bender, A. and Pringle, J.R. (1991) Use of a screen for synthetic lethal and multicopy suppresser mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Molecular and Cellular Biology, 11, 1295–305.

    PubMed  CAS  Google Scholar 

  • Berger, F. and Brownlee, C. (1993) Ratio confocal imaging of free cytoplasmic calcium gradients in polarising and polarised Fucus zygotes. Zygote, 1, 9–15.

    Article  PubMed  CAS  Google Scholar 

  • Betina, V., Micekova, D. and Nemec, P. (1972) Antimicrobial properties of cytochalasins and their alteration of fungal morphology. Journal of General Microbiology, 71, 343–9.

    Article  CAS  Google Scholar 

  • Bracker, C.E., Ruiz-Herrera, J. and Bartnicki-Garcia, S. (1976) Structure and transformation of chitin synthetase particles (chitosomes) during microfibril synthesis in vitro. Proceedings of the National Academy of Sciences of the USA, 73, 4570–4.

    Article  PubMed  CAS  Google Scholar 

  • Brennwald, P. and Novick, P. (1993) Interactions of three domains distinguishing the rasrelated GTP-binding proteins Yptl and Sec4. Nature, 362, 560–3.

    Article  PubMed  CAS  Google Scholar 

  • Brownlee, C. and Wood, J.W. (1986) A gradient of cytoplasmic free calcium in growing rhizoid cells of Fucus serratus. Nature, 320, 624–6.

    Article  CAS  Google Scholar 

  • Burnett, J.H. (1979) Aspects of the structure and growth of hyphal walls, in Fungal Walls and Hyphal Growth, (eds J.H. Burnett and A.P.J. Trinci) Cambridge University Press, Cambridge, pp. 1–15.

    Google Scholar 

  • Chant, J. and Herskowitz, I. (1991) Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway. Cell, 65, 1203–12.

    Article  PubMed  CAS  Google Scholar 

  • Chant, J., Corrado, K., Pringle, J.R. and Herskowitz, I. (1991) Yeast BUD5, encoding putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1. Cell, 65, 1213–24.

    Article  PubMed  CAS  Google Scholar 

  • Chenevert, J., Corrado, K., Bender, A. et al. (1992) A yeast gene (BEMI) necessary for cell polarization whose gene product contains two SH3 domains. Nature, 356, 77–9.

    Article  PubMed  CAS  Google Scholar 

  • Cho, C., Harold, F.M. and Scheurs, W.J.A. (1991) Electric and ionic dimensions of apical growth in Achlya hyphae. Experimental Mycology, 15, 34–43.

    Article  Google Scholar 

  • Collinge, A.J., and Trinci, A.P.J. (1974) Hyphal tips of wild type and spreading colonial mutants of Neurospora crassa. Archives of Microbiology, 99, 353–68.

    Article  PubMed  CAS  Google Scholar 

  • Costigan, C., Gehrung, S. and Synder, M. (1992) A synthetic lethal screen identifies SLK1, a novel protein kinase homolog implicated in yeast cell morphogenesis and cell growth. Molecular and Cellular Biology, 12, 1162–78.

    PubMed  CAS  Google Scholar 

  • Crombie, T., Gow, N.A.R. and Gooday, G.W. (1990) Influence of applied electrical fields on yeast and hyphal growth of Candida albicans. Journal of General Microbiology, 136, 311–17.

    Article  PubMed  CAS  Google Scholar 

  • Davenport, R.W. and Kater, S.B. (1992) Local increases in intracellular calcium elicit local filopodial responses in helisoma neuronal growth cones. Neuron, 9, 405–416.

    Article  PubMed  CAS  Google Scholar 

  • De Silva, L.R., Youatt, J., Gooday, G.W. and Gow, N.A.R. (1992) Inwardly directed ionic currents of Allomyces macrogynus and other water moulds indicate sites of proton-driven nutrient transport but are incidental to tip growth. Mycological Research, 96, 925–31.

    Article  Google Scholar 

  • De Vries, S.C. and Wessels, J.G.H. (1982) Polarized outgrowth of hyphae by constant electrical fields during reversion of Schizophyllum commune protoplasts. Experimental Mycology, 6, 95–8.

    Article  Google Scholar 

  • Dicker, J.W. and Turian, G. (1990) Calcium deficiencies and apical hyperbranching in wild-type and the ‘frost’ and’ spray’ morphological mutants of Neurospora crassa. Journal of General Microbiology, 136, 1413–20.

    Article  CAS  Google Scholar 

  • Drubin, D.G. (1991) Development of cell polarity in budding yeast. Cell, 65, 1093–6.

    Article  PubMed  CAS  Google Scholar 

  • Drubin, D.G., Miller, K.G. and Botstein, D. (1988) Yeast actin-binding proteins: evidence for a role in morphogenesis. Journal of Cell Biology, 107, 2551–61.

    Article  PubMed  CAS  Google Scholar 

  • Drubin, D.G., Mullholland, J., Zhu, Z. and Botstein, D. (1990) Homology of a yeast actin-binding protein to signal trasnduction proteins and myosin-I. Nature, 343, 288–90.

    Article  PubMed  CAS  Google Scholar 

  • Eamus, D. and Jennings, D.H. (1986) Turgor and fungal growth: studies on water relations of mycelia of Serpula lacrimans and Phallus impudicus. Transactions of the British Mycological Society, 86, 527–35.

    Article  Google Scholar 

  • Eamus, D., Thompson, W., Cairney, J.W.G. and Jennings, D.H. (1985) Internal structure and hydraulic conductivity of basidiomycete translocating organs. Journal of Experimental Botany, 36, 1110–16.

    Article  Google Scholar 

  • Enos, A.P. and Morris, N.R. (1990) Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans. Cell, 60, 1019–27.

    Article  PubMed  CAS  Google Scholar 

  • Finegold, A.A., Johnson, D.I., Fransworth, C.C. et al. (1991) Protein geranylgeranylytransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product. Proceedings of the National Academy of Sciences of the USA, 88, 4448–52.

    Article  PubMed  CAS  Google Scholar 

  • Flescher, E.G., Madden, K. and Snyder, M. (1993) Components required for cytokinesis are important for bud site selection. Journal of Cell Biology, 122, 373–86.

    Article  PubMed  CAS  Google Scholar 

  • Garrill, A., Lew, R.R. and Heath, I.B. (1992) Stretch-activated Ca2+ and Ca2+-activated K+ channels in the hyphal tip plasma membrane of the oomycete Saprolegnia ferax. Journal of Cell Science, 101, 721–30.

    CAS  Google Scholar 

  • Garrill, A., Jackson, S.L., Lew, R.R. and Heath, I.B. (1993) Ion channel activity and tip growth: tip localised stretch-activated channels generate an essential Ca2+ gradient in the oomycete Saprolegnia ferax. European Journal of Cell Biology, 60, 358–65.

    PubMed  CAS  Google Scholar 

  • Gehrung, S. and Snyder, M. (1990) The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating. Journal of Cell Biology, 111, 1451–64.

    Article  PubMed  CAS  Google Scholar 

  • Girbardt, M. (1969) Die ultrastruktur der apikalregion von pilzhyphen. Protoplasma, 67, 413–41.

    Article  Google Scholar 

  • Gooday, G.W. (1971) An autoradiographic study of hyphal growth of some fungi. Journal of General Microbiology, 67, 125–33.

    Article  CAS  Google Scholar 

  • Gooday, G.W. (1979) Chitin synthesis and differentiation in Coprinus cinereus, in Fungal Walls and Hyphal Growth (eds J.H. Burnett and A.P.J. Trinci), Cambridge University Press, Cambridge, pp. 203–23.

    Google Scholar 

  • Gooday, G.W. (1993) Cell envelope diversity and dynamics in yeasts and filamentous fungi. Journal of Applied Bacteriology Symposium Supplement, 74, 12S–20S.

    Article  Google Scholar 

  • Gooday, G.W. and Trinci, A.P.J. (1980) Wall structure and biosynthesis in fungi, in The Eukaryotic Microbial Cell, Society for General Microbiology Symposium, vol.30 (eds G.W. Gooday, D. Lloyd and A.P.J. Trinci), Cambridge University Press, Cambridge, pp. 207–51.

    Google Scholar 

  • Gow, N.A.R. (1984) Transhyphal electrical currents in fungi. Journal of General Microbiology, 130, 3313–18.

    PubMed  CAS  Google Scholar 

  • Gow, N.A.R. (1987) Polarity and branching in fungi induced by electrical fields, in Spatial Organization in Eukaryotic Microbes (Volume 23 of Special publications of the Society for General Microbiology) (eds R.K. Poole and A.P.J. Trinci). IRL Press, Oxford, pp. 25–41.

    Google Scholar 

  • Gow, N.A.R. (1989a) Control of the extension of the hyphal apex. Current Topics in Medical Mycology, 3, 109–52.

    Article  PubMed  CAS  Google Scholar 

  • Gow, N.A.R. (1989b) The circulating ionic currents of microorganisms. Advances in Microbial Physiology, 30, 89–123.

    Article  PubMed  CAS  Google Scholar 

  • Gow, N.A.R. and Gooday, G.W. (1987) Effects of antheridiol on growth, branching and electrical currents of Achlya ambisexualis. Journal of General Microbiology, 133, 3531–35.

    CAS  Google Scholar 

  • Gow, N.A.R., Kropf, D.L. and Harold, F.M. (1984) Growing hyphae of Achlya bisexualis generate a longitudinal pH gradient in the surrounding medium. Journal of General Microbiology, 130, 2967–74.

    PubMed  CAS  Google Scholar 

  • Gow, N.A.R., Miller, P.F.P. and Gooday, G.W. (1992) Life at the apex: growth of the hyphal tip. Journal of Chemical Technology and Biotechnology, 56, 217–19.

    Google Scholar 

  • Grove, S.M. and Bracker, C.E. (1970) Protoplasmic organization of hyphal tips among fungi: vesicles and Spitzenkorper. Journal of Bacteriology, 104, 989–1009.

    PubMed  CAS  Google Scholar 

  • Grove, S.M., Bracker, C.E. and Morre, D.J. (1970) An ultrastructural basis for hyphal tip growth in Pythium ultimum. American Journal of Botany, 57, 245–66.

    Article  Google Scholar 

  • Harold, F.M. (1990) To shape a cell: an inquiry into the causes of morphogenesis of microorgansims. Microbiological Reviews, 54, 381–431.

    PubMed  CAS  Google Scholar 

  • Heath, I.B. (1987) Preservation of a labile cortical array of actin microfilaments in growing hyphal tips of the fungus Saprolegnia ferax. European Journal of Cell Biology, 44, 10–16.

    Google Scholar 

  • Heath, I.B. (1990) The roles of actin in tip growth in fungi. International Review of Cytology, 123, 95–127.

    Article  CAS  Google Scholar 

  • Heath, I.B. and Kaminskyj, S.G.W. (1989) The organisation of tip-growth related organelles and microtubules revealed by quantitative analysis of freeze-substituted oomycete hyphae. Journal of Cell Science, 93, 41–52.

    Google Scholar 

  • Heath, I.B., Rethoret, K. Arsenault, A.L. and Ottensmeyer, F.P. (1985) Improved preservation of the form and contents of wall vesicles and the Golgi apparatus in freeze substituted hyphae of Saprolegnia. Protoplasma, 128, 81–93.

    Article  Google Scholar 

  • Herr, F.B. and Heath, M.C. (1982) The effects of antimicrotubule agents on organelle positioning in the cowpea rust fungus, Uromyces phaseoli var. vignae. Experimental Mycology, 6, 15–24.

    Article  CAS  Google Scholar 

  • Hoch, H.C. and Staples, R.C. (1985) The microtubule cytoskeleton in hyphae of Uromyces phaseoli germlings: its relationship to the region of nucleation and to the F-actin cytoskeleton. Protoplasma, 124, 112–22.

    Article  CAS  Google Scholar 

  • Hoch, H.C., Staples, R.C., Whitehead, B. et al., (1987) Signaling for growth orientation and cell differentiation by surface topography in Uromyces. Science, 235, 1659–62.

    Article  PubMed  CAS  Google Scholar 

  • Horwitz, B.A., Weisenseel, M.H., Dorm, A. and Gressel, J. (1984) Electric currents around growing Trichoderma hyphae: before and after photoinduction of conidiation. Plant Physiology, 74, 912–16.

    Article  PubMed  CAS  Google Scholar 

  • Howard, RJ. (1981) Ultrastructural analysis of hyphal tip cell growth in fungi: Spitzenkörper, cytoskeleton and endomembranes after freeze-substitution. Journal of Cell Science, 48, 89–103.

    PubMed  CAS  Google Scholar 

  • Howard, R.J and Aist, J.R. (1977) Effects of MBC on hyphal tip organisation, growth and mitosis of Fusarium accuminatum, and their antagonism by D2O. Protoplasma, 92, 195–210.

    Article  PubMed  CAS  Google Scholar 

  • Howard, R.J and Aist, J.R. (1980) Cytoplasmic microtubules and fungal morphogenesis: ultrastructural effects of methyl benzimidazole-2-ylcarbamate determined by freeze-substitution of hyphal tip cells. Journal of Cell Biology, 87, 55–64.

    Article  PubMed  CAS  Google Scholar 

  • Howard, R.J. and O’Donnell, K. (1987) Freeze substitution of fungi for cytological analysis. Experimental Mycology, 11, 250–69.

    Article  Google Scholar 

  • Huffaker, T.C., Thomas, J.H. and Botstein, D. (1988) Diverse effects of (3-tubulin mutations on microtubule formation and function. Journal of Cell Biology, 106, 1997–2010.

    Article  PubMed  CAS  Google Scholar 

  • Humphreys, A.M. and Gooday, G.W. (1984). Properties of chitinase activities from Mucor mucedo: evidence for a membrane-bound zymogenic form. Journal of General Microbiology, 130, 1359–66.

    CAS  Google Scholar 

  • Jackson, S.L. and Heath, I.B. (1989) Effects of exogenous calcium ions on tip growth, intracellular Ca2+ concentration, and actin arrays in hyphae of the fungus Saprolegnia ferax. Experimental Mycology, 13, 1–12.

    Article  Google Scholar 

  • Jackson, S.L. and Heath, I.B. (1990a) Visualization of actin arrays in growing hyphae of the fungus Saprolegnia ferax. Protoplasma, 154, 66–70.

    Article  Google Scholar 

  • Jackson, S.L. and Heath, I.B. (1990b) Evidence that actin reinforces the extensible hyphal apex of the oomycete Saprolegnia ferax. Protoplasma, 157, 144–53.

    Article  Google Scholar 

  • Jackson, S.L. and Heath, I.B. (1992) UV microirradiations elicit Ca2+ dependent apex-directed cytoplasmic contractions in hyphae. Protoplasma, 170, 46–52.

    Article  Google Scholar 

  • Jackson, S.L. and Heath, I.B. (1993a) The dynamic behavior of cytoplsamic F-actin in growing hyphae. Protoplasma, 173, 23–34.

    Article  CAS  Google Scholar 

  • Jackson, S.L. and Heath, I.B. (1993b) Roles of calcium ions in hyphal tip growth. Microbiological Reviews, 57, 367–82.

    PubMed  CAS  Google Scholar 

  • Jacobs, C.W., Adams, A.E.M., Szaniszlo, PJ. and Pringle, J.R. (1988) Functions of microtubules in the Saccharomyces cerevisiae cell cycle. Journal of Cell Biology, 107, 1409–26.

    Article  PubMed  CAS  Google Scholar 

  • Jaffe, L.F. and Nuccitelli, R. (1974) An ultrasensitive vibrating probe for measuring extracellular electrical currents. Journal of Cell Biology, 63, 614–28.

    Article  PubMed  CAS  Google Scholar 

  • Jaffe, L.F. (1977) Electrophoresis along cell membranes. Nature, 265, 600–2.

    Article  PubMed  CAS  Google Scholar 

  • Jennings, D.H. (1987) Translocation of solutes in fungi. Biological Reviews, 62, 215–43.

    Article  CAS  Google Scholar 

  • Johnson, B.F. (1965) Autoradiographic analysis of regional wall growth of yeast, Schizosaccharomyces pombe. Experimental Cell Research 39, 613–24.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, B.F. and Gibson, E.J. (1966) Autroradiographic analysis of regional wall growth of yeasts III, Saccharomyces cerevisiae. Experimental Cell Research, 41, 580–91.

    Article  PubMed  CAS  Google Scholar 

  • Johnston, G.C., Prendergast, J.A. and Singer, R.A. (1991) The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles. Journal of Cell Biology, 113, 539–51.

    Article  PubMed  CAS  Google Scholar 

  • Kaminskyj, S.G.W., Garrill, A. and Heath, I.B. (1992) The relation between turgor and tip growth in Saprolegnia ferax: turgor is necessary, but not sufficient to explain apical extension rates. Experimental Mycology, 16, 64–75.

    Article  Google Scholar 

  • Kilmartin, J.V. and Adams, A.E.M. (1984) Structural rearrangments of tubulin and actin during the cell cycle of the yeast Saccharomyces. Journal of Cell Biology, 98, 922–33.

    Article  PubMed  CAS  Google Scholar 

  • Koch. A.L. (1982) The shape of hyphal tips of fungi. Journal of General Microbiology, 128, 947–51.

    Google Scholar 

  • Knight, H., Trewavas, A.J. and Read, N.D. (1993) Confocal microscopy of living fungal hyphae microinjected with Ca2+-sensitive fluorescent dyes. Mycological Research, 97, 1505–15.

    Article  Google Scholar 

  • Kropf, D.L. (1986) Electrophysiological studies of Achlya hyphae: ionic currents studies by intracellular recording potentials. Journal of Cell Biology, 102, 1209–16.

    Article  PubMed  CAS  Google Scholar 

  • Kropf, D.L. and Quatrano, R.S. (1987) Localization of membrane-associated calcium development of fucoid algae using chlorotetracycline. Planta, 171, 158–70.

    Article  CAS  Google Scholar 

  • Kropf, D.L., Lupa, M.D., Caldwell, J.C. and Harold, F.M. (1983) Cell polarity: endogenous ion currents precede and predict branching in the water mold Achlya. Science, 220, 1385–87.

    Article  PubMed  CAS  Google Scholar 

  • Kropf, D.L., Caldwell, J.C., Gow, N.A.R. and Harold, F.M. (1984) Transcellular ion currents in the water mould Achlya. Amino acid symport as a mechanism of current entry. Journal of Cell Biology, 99, 486–96.

    Article  PubMed  CAS  Google Scholar 

  • Kwon, Y.H., Hoch, H.C. and Staples, R.C. (1991) Cytoskeletal organization in Uromyces urediospore germling apices during appressorium formation. Protoplasma, 165, 37–50.

    Article  Google Scholar 

  • Lever, M.,C., Robertson, B.E.M., Buchan, A.D.B. et al (1993) pH and Ca2+ dependent galvanotropism of filamentous fungi: implications and mechanisms. Mycological Research, 98, 301–6.

    Article  Google Scholar 

  • Lillie, S.H. and Brown, S.S. (1992) Suppression of a myosin defect by a kinesin-related gene. Nature, 256, 358–61.

    Article  Google Scholar 

  • Liu, H. and Bretscher, A. (1989) Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton. Cell, 57, 233–42.

    Article  PubMed  CAS  Google Scholar 

  • Luard, E. (1982a) Accumulation of intracellular solutes by two filamentous fungi in response to growth at low steady state osmotic potential. Journal of General Microbiology, 128, 2563–74.

    CAS  Google Scholar 

  • Luard, E. (1982b) Effect of osmotic shock on some intracellular solutes in two filamentous fungi. Journal of General Microbiology, 128, 2575–81.

    CAS  Google Scholar 

  • Luard, E. (1982c) Growth and accumulation of solutes by Phytophthora cinnamomi and other lower fungi in response to changes in external osmotic potential. Journal of General Microbiology, 128, 2583–90.

    CAS  Google Scholar 

  • Luard, E. and Griffin, D.M. (1981) Effect of water potential on fungal growth and turgor. Transactions of the British Mycological Society, 76, 33–40.

    Article  Google Scholar 

  • Madden, K., Costigan, C. and Snyder, M. (1992) Cell polarity and morphogenesis in Saccharomyces cerevisiae. Trends in Cell Biology, 2, 22–9.

    Article  PubMed  CAS  Google Scholar 

  • Markham, P., Robson, G.D., Bainbridge, B.W. and Trinci, A.P.J. (1993) Choline: its role in the growth of filamentous fungi and the regulation of mycelial morphology. FEMS Microbiology Reviews, 104, 287–300.

    Article  CAS  Google Scholar 

  • Marks, J. and Hyams, J.S. (1985) Localization of F-actin through the cell division cycle of Schizosaccharomyces pombe. European Journal of Cell Biology, 39, 27–32.

    Google Scholar 

  • Martinez-Cadena, G. and Ruiz-Herrera, J. (1987) Activation of chitin synthetase from Phycomyces blakesleeanus by calcium and calmodulin. Archives of Microbiology, 148, 280–5.

    Article  CAS  Google Scholar 

  • Matsuzaki, F., Matsumoto, S. and Yahara, I. (1988) Truncation of the carboxy-terminal domain of yeast β-tubulin causes temperature-sensitive growth and hypersensitivity to antimitotic drugs. Journal of Cell Biology, 107, 14427–35.

    Article  Google Scholar 

  • McGillivray, A.M. and Gow, N.A.R. (1986) Applied electrical fields polarize the growth of mycelial fungi. Journal of General Microbiology, 132, 2515–25.

    Google Scholar 

  • McGillivray, A.M. and Gow, N.A.R. (1987) The transhyphal electrical current of Neurospora crassa is carried principally by protons. Journal of General Microbiology, 133, 1875–81.

    Google Scholar 

  • McKerracher, L.J. and Heath, I.B. (1985) Microtubules around migrating nuclei in conventionally-fixed and freeze-substituted cells. Protoplasma, 125, 162–72.

    Article  Google Scholar 

  • McKerracher, L.J. and Heath, I.B. (1986a) Fungal nuclear behaviour analysed by ultraviolet microbeam irradiation. Cell Motility and the Cytoskeleton, 6, 35–47.

    Article  Google Scholar 

  • McKerracher, L.J. and Heath, I.B. (1986b) Polarized cytoplasmic movement and inhibition of saltations induced by calcium-mediated effects of microbeams in fungal hyphae. Cell Motility and the Cytoskeleton, 6, 136–45.

    Article  CAS  Google Scholar 

  • McKerracher, L.J. and Heath, I.B. (1987) Cytoplasmic migration and intracellular movements of organelles during tip growth of fungal hyphae. Experimental Mycology, 11, 79–100.

    Article  Google Scholar 

  • Merson-Davies, L. and Odds, F.C. (1992) Expansion of the Candida albicans cell envelope in different morphological forms of the fungus. Journal of General Microbiology, 138, 461–6.

    Article  PubMed  CAS  Google Scholar 

  • Miller, D.R., Callahan, D.A., Gross, D.J. and Hepler, P. (1992) Free Ca2+ gradient in growing pollen tubes of Lilium. Journal of Cell Science, 101, 7–12.

    CAS  Google Scholar 

  • Mitchison, J.M. and Nurse, P. (1985) Growth in cell length in the fission yeast Schizosaccharomyces pombe. Journal of Cell Science, 75, 357–76.

    PubMed  CAS  Google Scholar 

  • Money, N.P. (1990) Measurement of turgor pressure. Experimental Mycology, 14, 416–25.

    Article  Google Scholar 

  • Money, N.P. and Harold, F.M. (1992) Extension of the water mold Achlya: interplay of turgor and wall strength. Proceedings of the National Academy of Sciences of the USA, 89, 4245–9.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, W.J. (1992) Regulation of cell surface polarity from bacteria to mammals. Science, 258, 948–55.

    Article  PubMed  CAS  Google Scholar 

  • Novick, P. and Botstein, D. (1985) Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell, 40, 405–16.

    Article  PubMed  CAS  Google Scholar 

  • Novick, P. and Brennwald, P. (1993) Friends and family: the role of the rab GTPases in vesicular traffic. Cell, 75, 597–601.

    Article  PubMed  CAS  Google Scholar 

  • Nuccitelli, R. (1990) Vibrating probe technique for studies of ion transport, in Noninvasive Techniques in Cell Biology, (eds J.K. Foskett and S. Grinstein), Wiley-Liss, New York, pp. 273–310.

    Google Scholar 

  • Oakley, B.R. and Morris, N.R. (1980) Nuclear movement is B-tubulin-dependent in Aspergillus nidulans. Cell, 19, 255–62.

    Article  PubMed  CAS  Google Scholar 

  • Oakley, B.R. and Rinehart, J.E. (1985) Mitochondria and nuclei move by different mechanisms in Aspergillus nidulans. Journal of Cell Biology, 101, 2392–7.

    Article  PubMed  CAS  Google Scholar 

  • Ohya, Y., Miyamoto, S., Ohsumi, Y. and Anraku, Y. (1986) Calcium-sensitive cls4 mutants of Saccharomyces cerevisiae with a defect in bud formation. Journal of Bacteriology, 165, 28–33.

    PubMed  CAS  Google Scholar 

  • Ohya, Y., Goebel, M., Goodman, L.E. et al. (1991) Yeast CAL1 is a structural and functional homologue to the DPR1 (RAM) gene involved in ras processing. Journal of Biological Chemistry, 266, 12356–60.

    PubMed  CAS  Google Scholar 

  • Onuma, E.K. and Hui, S.-W. (1988) Electric-field directed cell shape changes, displacement, and cytoskeletal reorganization are calcium dependent. Journal of Cell Biology, 106, 2067–75.

    Article  PubMed  CAS  Google Scholar 

  • Orida, N. and Poo, M.-M. (1978) Electrophoretic movement and localization of acetylcholine receptors in the embryonic muscle cell membrane. Nature, 275, 31–5.

    Article  PubMed  CAS  Google Scholar 

  • Pfyffer, G.E. and Rast, D.M. (1988) The polyol pattern of fungi as influenced by the carbohydrate nutrient source. New Phytologist, 109, 321–6.

    Article  CAS  Google Scholar 

  • Pfyffer, G.E. and Rast, D.M. (1989) Accumulation of acyclic polyols and trehalose as related to growth form and carbohydrate source in the dimorphic fungi Mucor rouxii and Candida albicans. Mycopathologia, 105, 25–33.

    Article  PubMed  CAS  Google Scholar 

  • Poo, M-M. (1981) In situ electrophoresis of membrane components. Annual Review of Biophysics and Bioengineering, 10, 245–76.

    Article  PubMed  CAS  Google Scholar 

  • Powers, S., Gonzales, E., Christensn, T. et al. (1991) Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant. Cell, 65, 1225–31.

    Article  PubMed  CAS  Google Scholar 

  • Rajnicek, A.M., McCaig, CD. and Gow, N.A.R. (1994) Electric fields induce curved growth of Enterobacter clocacae, Escherichia coli and Bacillus subtilis cells: impliations for mechanisms of galvanotropism and bacterial growth. Journal of Bacteriology, 176, 702–13.

    PubMed  CAS  Google Scholar 

  • Rast, D.R., Horsch, M., Furter, R. and Gooday, G.W. (1991) A complex chitinolytic system in exponentially growing mycelium of Mucor rouxii: properties and function. Journal of General Microbiology, 137, 2797–810.

    Article  PubMed  CAS  Google Scholar 

  • Read, N.D., Allan, W.T.G., Knight, H. et al. (1992) Imaging and measurement of cytosolic free calcium in plant and fungal cells. Journal of Microscopy, 166, 57–86.

    Article  CAS  Google Scholar 

  • Reinhardt, M.O. (1892) Das Wachstum der Pilzhphen. Ein Beitrag zur Kenntnis des Flächenwachstums vegetalischer Zellmembranen. Jahrbucher für Wissenschaftliche Botanik, 23, 479–566.

    Google Scholar 

  • Reiss, H-D. and Herth, W. (1979) Calcium gradients in tip growing plants visualized by chlorotetracycline-fluorescence. Planta, 146, 615–21.

    Article  CAS  Google Scholar 

  • Reissig, J.L. and Kinney, S.G. (1983) Calcium as a branching signal in Neurospora crassa. Journal of Bacteriology, 154, 1397–402.

    PubMed  CAS  Google Scholar 

  • Roberson, R.W. (1992) The actin cytoskeleton in hyphal cells of Sclerotium rolfsii. Mycologia, 84, 41–51.

    Article  CAS  Google Scholar 

  • Roberson, R.W. and Fuller, M.S. (1988) Ultrastructural aspects of the tip of Sclerotium rolfsii. preserved by freeze substitution. Protoplasma, 146, 143–9.

    Article  Google Scholar 

  • Robinson, K.R. (1985) The responses of cells to electrical fields: a review. Journal of Cell Biology, 101, 2023–7.

    Article  PubMed  CAS  Google Scholar 

  • Robson, G.D., Wiebe, M.G. and Trinci, A.P.J. (1991a) Involvement of Ca2+ in the regulation of hyphal extension and branching in Fusarium graminearum A3/5. Experimental Mycology, 15, 263–72.

    Article  CAS  Google Scholar 

  • Robson, G.D., Wiebe, M.G. and Trinci, A.P.J. (1991b) Low calcium concentrations induce increased branching in Fusarium graminearum. Mycological Research, 95, 561–5.

    Article  CAS  Google Scholar 

  • Robson, G.D., Wiebe, M.G. and Trinci, A.P.J. (1991c) Exogenous cAMP and cGMP modulate branching in Fusarium graminearum. Journal of General Microbiology, 137, 963–9.

    Article  PubMed  CAS  Google Scholar 

  • Roncal, T., Ugalde, U.O. and Irastorza, A. (1993) Calcium-induced conidiation in Penicillium cyclopium: calcium triggers cytosolic alkalinization at the hyphal tip. Journal of Bacteriology, 175, 879–86.

    PubMed  CAS  Google Scholar 

  • Ruiz-Herrera, J., Valenzuela, C., Martinez-Cadena, G. and Obregon, A. (1989) Alterations in the vesicular pattern of and wall growth of Phycomyces induced by the calcium ionophore A23197. Protoplasma, 148, 15–25.

    Article  Google Scholar 

  • Saunders, P.T. and Trinci, A.P.J. (1979) Determination of tip shape in fungal hyphae. Journal of General Microbiology, 110, 469–73.

    Article  Google Scholar 

  • Schmid, J. and Harold, F.M. (1988) Dual role for calcium ions in apical growth of Neurospora crassa. Journal of General Microbiology, 134, 2623–31.

    PubMed  CAS  Google Scholar 

  • Schreurs, W.J.A. and Harold, F.M. (1988) Transcellular proton current in Achlya bisexualis hyphae: relationship to polarized growth. Proceedings of the National Academy of Sciences of the USA, 85, 1534–8.

    Article  PubMed  CAS  Google Scholar 

  • Sietsma, J.H. and Wessels, J.G.H. (1979) Evidence for covalent linkages between chitin and (β-glucan in a fungal wall. Journal of General Microbiology, 114, 99–108.

    Article  CAS  Google Scholar 

  • Sietsma, J.H. and Wessels, J.G.H. (1981) Solubility of (1-→3)-β-D(1→ 6)-3-D-glucan in fungal walls: importance of presumed linkage between glucan and chitin. Journal of General Microbiology, 125, 209–21.

    PubMed  CAS  Google Scholar 

  • Sietsma, J.H., Sonnenberg, A.S.M. and Wessels, J.H. (1985) Localization by autoradiography of (1→3)-β-D(1→6)-β-linkages in a wall glucan during hyphal growth of Schizophyllum commune. Journal of General Microbiology, 131, 1331–7.

    CAS  Google Scholar 

  • Slayman, C.L. and Slayman, C.W. (1962) Measurements of membrane potential in Neurospora. Science, 136,876–7.

    Article  PubMed  CAS  Google Scholar 

  • Sonnenberg, A.S.M., Sietsma, J.H. and Wessels, J.G.H. (1982) Biosynthesis of alkali-insoluble cell-wall glucan in Schizophyllum commune protoplasts. Journal of General Microbiology, 128, 2667–74.

    CAS  Google Scholar 

  • Sonnenberg, A.S.M., Sietsma, J.H. and Wessels, J.G.H. (1985). Spatial and temporal differences in the synthesis of (1→3)-β-D(1→6)-β linkages in a wall glucan of Schizophyllum commune. Experimental Mycology, 9, 141–8.

    Article  CAS  Google Scholar 

  • Snyder, M. (1989) The SPA2 protein of yeast localizes to sites of cell growth. Journal of Cell Biology, 108, 1419–29.

    Article  PubMed  CAS  Google Scholar 

  • Snyder, M., Gehrung, S. and Page, B.D. (1991) Studies concerning the temporal and genetic control of cell polarity in Saccharomyces cerevisiae. Journal of Cell Biology, 114, 515–32.

    Article  PubMed  CAS  Google Scholar 

  • Staebell, M and Soil, D.R. (1985) Temporal and spatial differences in cell wall expansion during bud and mycelium formation in Candida albicans. Journal of General Microbiology, 131, 1467–80.

    PubMed  CAS  Google Scholar 

  • Stollberg, J. and Fraser, S.E. (1988) Acetylcholine receptors and concanavalin A-binding sites on cultured Xenopus muscle cells: electrophoresis, diffusion and aggregation. Journal of Cell Biology, 107, 1397–408.

    Article  PubMed  CAS  Google Scholar 

  • Stump, R.F., Robinson, K.R., Harold, R.L. and Harold, F.M. (1980) Endogenous electrical currents in the water mould Blastocladiella emersonii during growth and sporulation. Proceedings of the National Academy of Sciences of the USA, 77, 6673–7.

    Article  PubMed  CAS  Google Scholar 

  • Szaniszlo, P.J., Mendoza, L. and Karuppayil, S.M. (1993) Clues about chromoblastomycotic and other dermatiaceous fungal pathogens based on Wangiella as a model, in Dimorphic Fungi in Biology and Medicine, (eds H. Vanden Bossche, F.C. Odds and D. Kerridge) Plenum Press, New York, pp. 241–55.

    Chapter  Google Scholar 

  • Takeuchi, Y., Schmid, J., Caldwell, J.H. and Harold, F.M. (1988) Transcellular ion currents and extension of Neurospora crassa hyphae. Journal of Membrane Biology, 101, 33–41.

    Article  PubMed  CAS  Google Scholar 

  • Temperli, E., Roos, U-P. and Hohl, H.R. (1991) Germ tube growth and the microtubule cytoskeleton in Phytophthora infestans: effects of antagonists of hyphal growth, microtubule inhibitors, and ionophores. Mycological Research, 95, 611–17.

    Article  CAS  Google Scholar 

  • That, T.C.C.-T., Rossier, C., Barja, F. et al. (1988) Induction of multiple germ tubes in Neurospora crassa by antimicrotubulin agents. European Journal of Cell Biology, 46, 68–79.

    PubMed  CAS  Google Scholar 

  • Thomas, des S. and Muffins, J.T. (1967) Role of enzymatic wall softening in plant morphogenesis. Science, 156, 84–5.

    Article  CAS  Google Scholar 

  • Thompson-Coffe, C. and Zickler, D. (1992) Three microtubule-organising centers are required for ascus growth and sporulation in the fungus Sordaria macrospora. Cell Motility and the Cytoskeleton, 22, 257–73.

    Article  Google Scholar 

  • Tsien, R.W. and Tsien, R.Y. (1990) Calcium channels, stores, and oscillations. Annual Review of Cell Biology, 6, 715–60.

    Article  PubMed  CAS  Google Scholar 

  • Van Laere, A.J. (1988) Effect of electrical fields on polar growth of Phycomyces blakesleeanus. FEMS Microbiological Letters, 49, 111–16.

    Article  Google Scholar 

  • Vermeulen, C.A. and Wessels, J.G.H. (1984) Ultrastructural differences between wall apices of growing and non-growing hyphae of Schizophyllum commune. Protoplasma, 120, 123–31.

    Article  Google Scholar 

  • Vermeulen, C.A. and Wessels, J.G.H. (1986) Chitin biosynthesis by a fungal membrane preparation. Evidence for a transient non-crystalline state of chitin. European Journal of Biochemistry, 158, 411–15.

    Article  PubMed  CAS  Google Scholar 

  • Wessels, J.G.H. (1984) Apical hyphal wall extension: do lytic enzymes play a role?, in Microbial Cell Wall Synthesis and Autolysis (ed. C. Nombela) Elsevier, Amsterdam, pp. 31–42.

    Google Scholar 

  • Wessels, J.G.H. (1986) Cell wall synthesis in apical hyphal growth. International Review of Cytology, 104, 37–79.

    Article  CAS  Google Scholar 

  • Wessels, J.G.H. (1993) Wall growth, protein excretion and morphogenesis in fungi. New Phytologist, 123, 387–413.

    Article  Google Scholar 

  • Wessels, J.G.H., Sietsma, J.H. and Sonnenberg, A.S.M. (1983) Wall synthesis and assembly during hyphal morphogenesis in Schizophyllum commune. Journal of General Microbiology, 129, 1607–16.

    CAS  Google Scholar 

  • Wiebe, M.G., Robson, G.D., and Trinci, A.P.J. (1992) Evidence for the independent regulation of hyphal extension and branch initiation in Fusarium graminearum A3/5. FEMS Microbiology Letters, 90, 179–84.

    CAS  Google Scholar 

  • Wittekindt, E., Lamprecht, I. and Kraepelin, G. (1989) DC electrical fields induce polarization effects in the dimorphic fungus Mycotypha africana. Endocytobiosis and Cell Research, 6, 41–56.

    Google Scholar 

  • Woods and Duniway, J.M. (1986) Some effects of water potential on turgor, and respiration of Phytophthora cryptogea and Fusarium moniliforme. Phytopathology, 76, 1248–54.

    Article  Google Scholar 

  • Yaun, S. and Heath, I.B. (1991) Chlortetracycline staining patterns of growing hyphal tips of the oomycete Saprolegnia ferax. Experimental Mycology, 15, 91–102.

    Article  Google Scholar 

  • Yokoyama, K., Kaji, H., Nishimura, K. and Miyaji, M. (1990) The role of microfilaments and microtubules in apical growth and dimorphism of Candida albicans. Journal of General Microbiology, 136, 1067–75.

    Article  PubMed  CAS  Google Scholar 

  • Youatt, J. (1993) Calcium and microorganisms. Critical Reviews in Microbiology, 19, 83–97.

    Article  PubMed  CAS  Google Scholar 

  • Youatt, J. and McKinnon, I. (1993) Manganese (Mn2+) reverses the inhibition by EGTA of the growth of fungi. Microbios, 74, 181–6.

    CAS  Google Scholar 

  • Youatt, J., Gow, N.A.R. and Gooday, G.W. (1988) Bioelectric and biosynthetic aspects of cell polarity in Allomyces macrogynus. Protoplasma, 146, 118–26.

    Article  Google Scholar 

  • Zhou, X-L., Stumpf, A., Hoch, H.C. and Kung, C. (1991) A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces. Science, 253, 1415–17.

    Article  PubMed  CAS  Google Scholar 

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© 1995 Neil A.R. Gow and Geoffrey M. Gadd

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Gow, N.A.R. (1995). Tip Growth and Polarity. In: Gow, N.A.R., Gadd, G.M. (eds) The Growing Fungus. Springer, Dordrecht. https://doi.org/10.1007/978-0-585-27576-5_13

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