Abstract
Taurine is abundant in polymorphonuclear leukocytes (PMNs) where it reacts with PMN-derived hypochlorous acid to form taurine chloramine (Tau-NHCl), a substance that does not readily cross the cell membrane. When PMNs were stimulated in PBS lacking taurine, extracellular oxidant concentration was low, but the concentration increased 3–4 fold when 15 mM taurine was added, indicating that taurine lowers oxidant levels inside the cell. When Tau-NHCl was added to Jurkat cells in suspension, its half life was about 75 min. In contrast, membrane-permeable ammonia mono-chloramine NH2Cl) has a half life of only 6 min. Accordingly, NH2Cl oxidizes cytosolic proteins, such as I\upkappaB, and inhibits NF-κB activation, whereas Tau-NHCl exhibits no comparable effect. However, when NH4 + was added to the medium, Tau-NHCl oxidizes IκB and inhibits NF-κB activation, probably through oxidant transfer to NH4 + leading to NH2Cl formation. These results indicate that Tau-NHCl can serve as an oxidant reservoir, exhibiting either delayed oxidant effects or acting as an oxidant at a distant site.
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Ogino, T., Than, T.A., Hosako, M., Ozaki, M., Omori, M., Okada, S. (2009). Taurine Chloramine: A Possible Oxidant Reservoir. In: Azuma, J., Schaffer, S.W., Ito, T. (eds) Taurine 7. Advances in Experimental Medicine and Biology, vol 643. Springer, New York, NY. https://doi.org/10.1007/978-0-387-75681-3_47
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DOI: https://doi.org/10.1007/978-0-387-75681-3_47
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