The use of live recombinant Salmonella attenuated vaccine (RASV) encoding Yersinia proteins is a promising new approach for the vaccination against Yersinia pestis. We have tested the efficacy of 2 proteins, Psn and a portion of LcrV in protecting mice against virulent Yersinia pestis challenge. To remove the immunosuppressive properties of LcrV protein, the lcrV gene, without the TLR2 receptor sequence, was cloned into a β-lactamase secretion vector. Immunizations were performed with RSAV expressing LcrV or Psn. Challenge with a virulent Y. pestis strain was performed 4 weeks after the last immunization. Our results show that the truncated LcrV protein delivered by RASV is sufficient to afford a full protective immune response in a mouse model of bubonic plague and the Psn protein afforded partial protection in a non-optimized system. This finding should facilitate the design and development of a new generation of vaccines against Y. pestis.
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References
Brubaker, R.R. (2003) Interleukin-10 and inhibition of innate immunity to Yersiniae: roles of Yops and LcrV (V antigen). Infect. Immun. 71, 3673-3681.
Calhoun L.N. and Kwon Y.M. (2006) Salmonella-based plague vaccines for bioterrorism. J. Microbiol. Immunol. Infect. 39, 92-97.
Curtiss, R. and Kelly, SM. (1987) Salmonella typhimurium deletion mutants lacking ade-nylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic. Infect. Immun. 55, 3035-3043.
Fetherston, J.D, Bearden, S.W. and Perry, R.D. (1996) YbtA, an AraC-type regulator of the Yersinia pestis pesticin/yersiniabactin receptor. Mol. Microbiol. 22, 315-325.
Fetherston, J.D, Lillard, J.W., Jr. and Perry, RD. (1995) Analysis of the pesticin receptor from Yersinia pestis: role in iron-deficient growth and possible regulation by its siderophore. J. Bacteriol. 177, 1824-1833.
Fields, K.A., Nilles, M.L., Cowan, C. and Straley, S.C. (1999) Virulence role of V antigen of Yersinia pestis at the bacterial surface. Infect. Immun. 67; 5395-408.
Galan, J.E., Nakayama, K. and Curtiss, R. (1990) Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains. Gene 94, 29-35.
Galimand, M., Guiyoule, A., Gerbaud, G., Rasoamanana, B., Chanteau, S., Carniel, E. and Courvalin, P. (1997) Multidrug resistance in Yersinia pestis mediated by a transferable plasmid. N. Engl. J. Med. 337, 677-680.
Garmory, H.S., Griffin, K.F., Brown, K.A. and Titball, R.W. (2003) Oral immunisation with live aroA attenuated Salmonella enterica serovar Typhimurium expressing the Yersinia pestis V antigen protects mice against plague. Vaccine 21, 3051-3057.
Geoffroy, V.A., Fetherston, J.D., Perry, R.D. (2000) Yersinia pestis YbtU and YbtT are in-volved in synthesis of the siderophore yersiniabactin but have different effects on regula-tion. Infect. Immun. 68, 4452-4461.
Germanier, R. and Fürer, E. (1975) Isolation and characterization of Gal E mutant Ty 21a of Salmonella typhi: a candidate strain for a live, oral typhoid vaccine. J. Infect. Dis. 131, 553-558.
Guiyoule, A., Gerbaud, G., Buchrieser, C., Galimand, M., Rahalison, L., Chanteau, S., Courvalin, P. and Carniel, E. (2001) Transferable plasmid-mediated resistance to strepto-mycin in a clinical isolate of Yersinia pestis. Emerg. Infect. Dis. 7, 43-48.
Hill, J., Leary, S.E., Griffin, K.F., Williamson, E.D. and Titball, R.W. (1997) Regions of Yersinia pestis V antigen that contribute to protection against plague identified by passive and active immunization. Infect. Immun. 65, 4476-4482.
Kang, H.Y. and Curtiss, R. (2003) Immune responses dependent on antigen location in recombinant attenuated Salmonella typhimurium vaccines following oral immunization. FEMS Immunol. Med. Microbiol. 37, 99-104.
Kang, H.Y., Srinivasan, J. and Curtiss, R. (2002) Immune responses to recombinant pneumococcal PspA antigen delivered by live attenuated Salmonella enterica serovar Ty-phimurium vaccine. Infect. Immun. 70, 1739-1749.
Laemmli, U.K. (1970) Cleavage of structural protein during the assembly of the head bacte-riophage T4. Nature 227, 680-685.
Morton, M., Garmory, H.S., Perkins, S.D., O’Dowd, A.M., Griffin, K.F., Turner, A.K., Bennett, A.M. and Titball, R.W. (2004) A Salmonella enterica serovar Typhi vaccine ex-pressing Yersinia pestis F1 antigen on its surface provides protection against plague in mice. Vaccine 22, 2524-2532.
Motin, V.L., Nakajima, R., Smirnov, G.B. and Brubaker, R.R. (1994) Passive immunity to yersiniae mediated by anti-recombinant V antigen and protein A-V antigen fusion peptide. Infect. Immun. 62, 4192-4201.
Nakajima, R. and Brubaker, R.R. (1993) Association between virulence of Yersinia pestis and suppression of gamma interferon and tumor necrosis factor alpha. Infect. Immun. 61, 23-31.
Nakayama, K., Kelly, S.M. and Curtiss, R. (1988) Construction of an asd+expression-cloning vector: stable maintenance and high level expression of cloned genes in a Salmo-nella vaccine strain. Biotechnology 6, 693-697.
Overheim, K.A., Depaolo, R.W., Debord, K.L., Morrin, E.M., Anderson, D.M., Green, N.M., Brubaker, R.R., Jabri, B. and Schneewind, O. (2005) LcrV plague vaccine with altered immunomodulatory properties. Infect. Immun. 73, 5152-5159.
Oyston, P.C., Williamson, E.D., Leary, S.E., Eley, S.M., Griffin, K.F. and Titball, R.W. (1995) Immunization with live recombinant Salmonella typhimurium aroA producing F1 antigen protects against plague. Infect. Immun. 63, 563-568.
Perry, R.D., Balbo, P.B., Jones, H.A., Fetherston, J.D. and DeMoll, E. (1999) Yersiniabactin from Yersinia pestis: biochemical characterization of the siderophore and its role in iron transport and regulation. Microbiology 145, 1181-1190.
Perry, R.D. and Fetherston, J.D. (1997) Yersinia pestis - etiologic agent of plague. Clin. Microbiol. Rev. 10, 35-66.
Perry, R.D., Harmon, P.A., Bowmer, W.S. and Straley, S.C. (1986) A low-Ca2+ response operon encodes the V antigen of Yersinia pestis. Infect. Immun. 54, 428-434.
Perry, R.D., Straley, S.C., Fetherston, J.D., Rose, D.J., Gregor, J. and Blattner, F.R. (1998) DNA sequencing and analysis of the low-Ca2+-response plasmid pCD1 of Yesinia pestis KIM5. Infect. Immun. 66, 4611-4623.
Russell, P., Eley, S.M., Hibbs, S.E., Manchee, R.J., Stagg, A.J. and Titball, R.W. (1995) A comparison of Plague vaccine, USP and EV76 vaccine induced protection against Yersinia pestis in a murine model. Vaccine 13, 1551-6.
Sarker, M.R., Neyt, C., Stainier, I. and Cornelis, G.R. (1998) The Yersinia Yop virulon: LcrV is required for extrusion of the translocators YopB and YopD. J. Bacteriol. 180, 1207-1214.
Sing, A., Reithmeier-Rost, D., Granfors, K., Hill, J., Roggenkamp, A. and Heesemann, J. (2005) A hypervariable N-terminal region of Yersinia LcrV determines Toll-like receptor 2-mediated IL-10 induction and mouse virulence. PNAS 102, 16049-16054.
Sing, A., Rost, D., Tvardovskaia, N., Roggenkamp, A., Wiedemann, A., Kirschning, C.J., Aepfelbacher, M. and Heesemann, J. (2002) Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J. Exp. Med. 196, 1017-1024.
Titball, R.W., Howells, A.M., Oyston, P.C. and Williamson, E.D. (1997) Expression of the Yersinia pestis capsular antigen (F1 antigen) on the surface of an aroA mutant of Salmo-nella typhimurium induces high levels of protection against plague. Infect. Immun. 65, 1926-1930.
Titball, R.W. and Williamson, E.D. (2001) Vaccination against bubonic and pneumonic plague. Vaccine 19, 4175-4184.
Wang, R.F. and Kushner, S.R. (1991) Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100, 195-199.
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Branger, C.G., Curtiss III, R., Perry, R.D., Fetherston, J.D. (2007). Oral Vaccination with Different Antigens from Yersinia pestis KIM Delivered by Live Attenuated Salmonella Typhimurium Elicits a Protective Immune Response Against Plague. In: Perry, R.D., Fetherston, J.D. (eds) The Genus Yersinia. Advances In Experimental Medicine And Biology, vol 603. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72124-8_36
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