Abstract
Increasing efforts have been directed towards investigating the involvement of nitric oxide (NO) for normal kidney function. Recently, a crucial role of NO in the development of progressive renal dysfunction has been reported during diabetes and hypertension. Indirect estimation of renal NO production include urinary nitrite/nitrate measurements, but there are several disadvantages of indirect methods since production and bioavailability of NO rarely coincide. Thus, direct measurement of in vivo NO bioavailability is preferred, although these methods are more time consuming and require highly specialized equipment and knowledge. This review focuses on two techniques for in vivo measurement of bioavailable NO in the kidney. We have applied Whalen-type recessed NO microsensors for measurement of NO in the kidney cortex, whereas the hemoglobin-trapping technique seems to be more suitable for NO measurement in the renal medulla. Both methods are robust and reliable, and we discuss advantages and shortcomings of each method.
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References
B. C. Kone, and C. Baylis, Biosynthesis and homeostatic roles of nitric oxide in the normal kidney, Am J Physiol 272(F561-F578 (1997).
M. G. Salom, B. Arregui, L. F. Carbonell, F. Ruiz, J. L. Gonzalez-Mora, and F. J. Fenoy, Renal ischemia induces an increase in nitric oxide levels from tissue stores, Am J Physiol Regul Integr Comp Physiol 289(5), R1459-1466 (2005).
N. Miyata, A. P. Zou, D. L. Mattson, and A. W. Cowley, Jr., Renal medullary interstitial infusion of L-arginine prevents hypertension in Dahl salt-sensitive rats, Am J Physiol 275(5 Pt 2), R1667-1673 (1998).
R. Komers, and S. Anderson, Paradoxes of nitric oxide in the diabetic kidney, Am J Physiol Renal Physiol 284(6), F1121-F1137 (2003).
F. Palm, D. G. Buerk, P. O. Carlsson, P. Hansell, and P. Liss, Reduced nitric oxide concentration in the renal cortex of streptozotocin-induced diabetic rats: effects on renal oxygenation and microcirculation, Diabetes 54(11), 3282-3287 (2005).
A. P. Zou, and A. W. Cowley, Jr., Nitric oxide in renal cortex and medulla. An in vivo microdialysis study, Hypertension 29(1 Pt 2), 194-198 (1997).
M. Kakoki, H. S. Kim, W. J. Arendshorst, and D. L. Mattson, L-Arginine uptake affects nitric oxide production and blood flow in the renal medulla, Am J Physiol Regul Integr Comp Physiol 287(6), R1478-1485 (2004).
P. A. Ortiz, and J. L. Garvin, Role of nitric oxide in the regulation of nephron transport, Am J Physiol Renal Physiol 282(5), F777-84 (2002).
A. Deng, C. M. Miracle, J. M. Suarez, M. Lortie, J. Satriano, S. C. Thomson, K. A. Munger, and R. C. Blantz, Oxygen consumption in the kidney: Effects of nitric oxide synthase isoforms and angiotensin II, Kidney Int 68(2), 723-730 (2005).
C. G. Schnackenberg, Physiological and pathophysiological roles of oxygen radicals in the renal microvasculature, Am J Physiol Regul Integr Comp Physiol 282(2), R335-R342 (2002).
A. Koivisto, J. Pittner, M. Froelich, and A. E. Persson, Oxygen-dependent inhibition of respiration in isolated renal tubules by nitric oxide, nKidney Int 55(6), 2368-2375 (1999).
R. H. Boger, Asymmetric dimethylarginine (ADMA) modulates endothelial function–therapeutic implications, Vasc Med 8(3), 149-51 (2003).
C. T. Tran, J. M. Leiper, and P. Vallance, The DDAH/ADMA/NOS pathway, Atheroscler Suppl 4(4), 33-40 (2003).
R. H. Boger, Asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide synthase, explains the "L-arginine paradox" and acts as a novel cardiovascular risk factor, J Nutr 134(10 Suppl), 2842S-2847S; discussion 2853S (2004).
D. G. Buerk, C. E. Riva, and S. D. Cranstoun, Nitric oxide has a vasodilatory role in cat optic nerve head during flicker stimuli, Microvasc Res 52(1), 13-26 (1996).
W. J. Whalen, J. Riley, and P. Nair, A microelectrode for measuring intracellular PO2, J Appl Physiol 23(5), 798-801 (1967).
Y. Zhang, F. E. Samson, S. R. Nelson, and T. L. Pazdernik, Nitric oxide detection with intracerebral microdialysis: important considerations in the application of the hemoglobin-trapping technique, J Neurosci Methods 68(2), 165-173 (1996).
A. Balcioglu, and T. J. Maher, The measurement of nitric oxide release induced by kainic acid using a novel hemoglobin-trapping technique with microdialysis, Ann N Y Acad Sci 738(282-288 (1994).
F. Palm, P. Hansell, G. Ronquist, A. Waldenstrom, P. Liss, and P. O. Carlsson, Polyol-pathway-dependent disturbances in renal medullary metabolism in experimental insulin-deficient diabetes mellitus in rats, Diabetologia 47(7), 1223-1231 (2004).
C. Thorup, M. Kornfeld, J. M. Winaver, M. S. Goligorsky, and L. C. Moore, Angiotensin-II stimulates nitric oxide release in isolated perfused renal resistance arteries, Pflugers Arch 435(3), 432-434 (1998).
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Palm, F., Nordquist, L., Buerk, D.G. (2008). Nitric Oxide in The Kidney Direct measurements of bioavailable renal nitric oxide. In: Maguire, D.J., Bruley, D.F., Harrison, D.K. (eds) Oxygen Transport to Tissue XXVIII. Advances in Experimental Medicine and Biology, vol 599. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-71764-7_16
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DOI: https://doi.org/10.1007/978-0-387-71764-7_16
Publisher Name: Springer, Boston, MA
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