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Protocols for Measuring Biosurfactant Production in Microbial Cultures

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Hydrocarbon and Lipid Microbiology Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Microbial biosurfactants have wide structural and functional diversity which consequently requires the adoption of a range of techniques to investigate these amphiphilic molecules. Literature on the production and analytical detection of biosurfactants is overwhelmed with assertions of high yields for such products which are mostly over exaggerated estimates due to the use of flawed or inaccurate analytical techniques. In this chapter we focus on quantitative methods available to allow accurate estimates of production and yield data to be generated.

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References

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Acknowledgement

We would like to thank Dr Thomas J.P. Smyth and Mr Scot Funston for valuable comments and results from analytical methods.

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Authors and Affiliations

  1. School of Biomedical Sciences, University of Ulster, Coleraine, County Londonderry, BT521SA, Northern Ireland, UK

    Roger Marchant & Ibrahim M. Banat

Authors
  1. Roger Marchant
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  2. Ibrahim M. Banat
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Corresponding author

Correspondence to Ibrahim M. Banat .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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© 2014 Springer-Verlag Berlin Heidelberg

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Marchant, R., Banat, I.M. (2014). Protocols for Measuring Biosurfactant Production in Microbial Cultures. In: McGenity, T., Timmis, K., Nogales, B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2014_10

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  • DOI: https://doi.org/10.1007/8623_2014_10

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-49138-6

  • Online ISBN: 978-3-662-49140-9

  • eBook Packages: Springer Protocols

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