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Pancreatic Cancer Organotypic Models

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Three Dimensional Human Organotypic Models for Biomedical Research

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 430))

Abstract

Pancreatic cancer has a dismal prognosis for patients due to late diagnosis and ineffective treatment options. There is a desperate need for more accurate disease models to enable improved therapies and diagnostic tests to reach the clinic. Pancreatic tumours have a high content of desmoplastic stroma, which forms a stiff, hypoxic tumour mass and contributes significantly to tumour development and metastatic spread. Therefore, 2D cell culture is not sufficient for understanding the complex biology of this disease. 3D in vitro models offer a more representative method of culturing cells for research in the laboratory. There are many different 3D models that can be used in research, organoids formed from patient or murine tumours are grown embedded in collagen or matrigel matrices, giving the potential for screening treatment options and personalised therapy in the future. Also, organotypic models using pancreatic cancer cell lines and stromal cells can be easily manipulated to study different aspects of pancreatic cancer and new therapeutic options in the laboratory. There are new emerging pancreatic cancer 3D models being developed, including microchip technology or synthetic scaffolds instead collagen and matrigel. All of these 3D culturing methods give an advantage over traditional 2D cell culture and could lead to improved understanding of this disease, translating to a better prognosis for patients in the clinic.

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Authors and Affiliations

  1. Barts Cancer Institute, London, UK

    Abigail Coetzee, Richard Grose & Hemant Kocher

Authors
  1. Abigail Coetzee
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  2. Richard Grose
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  3. Hemant Kocher
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Corresponding author

Correspondence to Abigail Coetzee .

Editor information

Editors and Affiliations

  1. GSK Vaccines Research, Siena, Italy

    Fabio Bagnoli

  2. GSK Vaccines Research, Siena, Italy

    Rino Rappuoli

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Coetzee, A., Grose, R., Kocher, H. (2019). Pancreatic Cancer Organotypic Models. In: Bagnoli, F., Rappuoli, R. (eds) Three Dimensional Human Organotypic Models for Biomedical Research. Current Topics in Microbiology and Immunology, vol 430. Springer, Cham. https://doi.org/10.1007/82_2019_155

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