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Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases

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Induced Pluripotent Stem Cells and Human Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2549))

Abstract

Here, we describe a protocol for reprogramming of bone marrow–derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease.

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Acknowledgments

This work was supported by a grant from The Scientific and Technological Research Council of Turkey (TÜBİTAK), project no. 214S071.

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Authors and Affiliations

  1. Koç University—Is Bank Center for Infectious Diseases, Istanbul, Turkey

    Gülen Güney-Esken

  2. Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, Ankara, Turkey

    Fatima Aerts-Kaya

  3. Hacettepe University Center for Stem Cell Research and Development, Ankara, Turkey

    Fatima Aerts-Kaya

Authors
  1. Gülen Güney-Esken
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  2. Fatima Aerts-Kaya
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Corresponding author

Correspondence to Fatima Aerts-Kaya .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Güney-Esken, G., Aerts-Kaya, F. (2021). Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_452

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  • DOI: https://doi.org/10.1007/7651_2021_452

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2584-2

  • Online ISBN: 978-1-0716-2585-9

  • eBook Packages: Springer Protocols

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