Abstract
Tissue engineering techniques to generate a graft ex vivo is an exciting field of research. In particular, the use of biological scaffolds has shown to be promising in a clinical setting. In this approach, decellularized donor scaffolds are obtained following detergent-based enzymatic treatment to remove donor cells and subsequently repopulated with recipient specific cells. Herein, we describe our bioreactor-based partial decellularization approach to generate hybrid tracheal grafts. Using a short detergent-based treatment with sodium dodecyl sulfate (SDS), we remove the epithelium and maintain the structural integrity of the donor grafts by keeping the cartilage alive. The following will be a step-by-step description of the bioreactor system setup and partial decellularization protocol to obtain a de-epithelialized tracheal graft.
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Acknowledgments
This work was supported in part of the University of Toronto’s Medicine by Design initiative, which receives funding from the Canada First Research Excellence Fund (CFREF; C1TPA-2016-18).
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Marin-Araujo, A.E., Haykal, S., Karoubi, G. (2021). Bioreactor-Based De-epithelialization of Long-Segment Tracheal Grafts. In: Turksen, K. (eds) Bioreactors in Stem Cell Biology. Methods in Molecular Biology, vol 2436. Humana, New York, NY. https://doi.org/10.1007/7651_2021_431
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DOI: https://doi.org/10.1007/7651_2021_431
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2017-5
Online ISBN: 978-1-0716-2018-2
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