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3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells

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Induced Pluripotent Stem (iPS) Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2454))

Abstract

The keratinocytes are predominant cells in the epidermis of the human skin. To assess the cellular response of the keratinocytes to the genotoxic stress, we derived the skin keratinocytes from human induced pluripotent stem cells (iPSCs). Furthermore, three-dimensional (3D) organoid culture method is powerful tool to analyze the organ and tissue response against the genotoxic stress. Here we describe the method of 3D organoid culture using skin keratinocytes derived from human iPSCs.

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Acknowledgments

Authors thank to Yoshihisa Matsumoto, Munekazu Kuge, and Matsumoto laboratory member for critical discussion, Masato Nikaido for technical assistance. This work was supported by The Uehara Memorial Foundation [to MS]; Takeda Science Foundation [to MS]; Kato Memorial Bioscience Foundation [to MS]; Japan Atomic Energy Agency [to MS]; Chubu Electric Power [to MS].

Author information

Authors and Affiliations

  1. Cosmetic R&D Department, Takara Belmont Corp., Tokyo, Japan

    Tomoko Miyake

  2. Laboratory for Advanced Nuclear Energy, Institute of Innovative Research, Tokyo Institute of Technology, Tokyo, Japan

    Mikio Shimada

Authors
  1. Tomoko Miyake
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  2. Mikio Shimada
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Corresponding author

Correspondence to Mikio Shimada .

Editor information

Editors and Affiliations

  1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

    Andras Nagy

  2. Ottawa, ON, Canada

    Kursad Turksen

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Miyake, T., Shimada, M. (2021). 3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2021_357

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  • DOI: https://doi.org/10.1007/7651_2021_357

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2118-9

  • Online ISBN: 978-1-0716-2119-6

  • eBook Packages: Springer Protocols

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