Abstract
Drug delivery to the brain is a tremendous problem for the academic society and the industry. One solution with a huge potential is to use endocytic receptors as carriers. Here we describe how endocytic activity and subcellular trafficking of a specific receptor in brain endothelial cells can be characterized in three steps. (1) Labeling, endocytosis, and trafficking of a specific receptor at given time points in a pulse-chase experiment. (2) Fixed antibody labeling and co-staining of subcellular markers for image acquisition. (3) Analysis and quantification of co-localization between the receptor and subcellular markers in ImageJ.
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Holst, M.R., Nielsen, S.S.E., Nielsen, M.S. (2020). Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 2367. Humana, New York, NY. https://doi.org/10.1007/7651_2020_312
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DOI: https://doi.org/10.1007/7651_2020_312
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1672-7
Online ISBN: 978-1-0716-1673-4
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