Abstract
Myocardial fibrosis is a hallmark of cardiac remodeling, which can progressively lead to heart failure, a leading cause of death worldwide. The effector cells of fibrosis in the heart are cardiac fibroblasts (CFs). There is currently no effective therapeutic strategy clinically available to specifically attenuate maladaptive responses of CFs. Large-scale applications such as high-throughput drug screening are difficult due to the limited availability of human primary CFs, thus limiting the development of future treatments. Here, we describe a robust induction protocol that can be used to generate a scalable, consistent, genetically defined source of quiescent CFs from human induced pluripotent stem cells for cardiac fibrosis modeling, drug discovery, and tissue engineering.
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Acknowledgments
This work was supported by research grants from: NIH R01 HL113006, R01 HL123968, R01 HL141371, R01 HL141851, and AHA 17MERIT33610009 (JCW).
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Zhang, H., Shen, M., Wu, J.C. (2020). Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2020_300
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DOI: https://doi.org/10.1007/7651_2020_300
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Publisher Name: Humana, New York, NY
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