Abstract
Baculovirus expression vector system (BEVS) is an established technology for recombinant protein expression in insect cells. Further, BEVS-mediated gene transduction of mammalian cells (BacMam) is emerging as a technique for high level recombinant protein expression in mammalian cells. Here, we describe generic method in using BEVS as a BacMam for rapid recombinant protein expression in mammalian cells.
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Acknowledgment
This work was supported by Envirotransgene® Global, Chennai, India. Authors thank infrastructural support from VIT Vellore and Bannari Amman Institute of Technology.
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Thimiri Govinda Raj, D.B., Khan, N.A., Venkatachalam, S., Arumugam, S. (2019). BacMam System for Rapid Recombinant Protein Expression in Mammalian Cells. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 2125. Humana, New York, NY. https://doi.org/10.1007/7651_2019_249
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DOI: https://doi.org/10.1007/7651_2019_249
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