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Gastrointestinal Epithelial Organoid Cultures from Postsurgical Tissues

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Organoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1576))

Abstract

An organoid is a cellular structure three-dimensionally (3D) cultured from self-organizing stem cells in vitro, which has a cell population, architectures, and organ specific functions like the originating organs. Recent advances in the 3D culture of isolated intestinal crypts or gastric glands have enabled the generation of human gastrointestinal epithelial organoids. Gastrointestinal organoids recapitulate the human in vivo physiology because of all the intestinal epithelial cell types that differentiated and proliferated from tissue resident stem cells. Thus far, gastrointestinal organoids have been extensively used for generating gastrointestinal disease models. This protocol describes the method of isolating a gland or crypt using stomach or colon tissue after surgery and establishing them into gastroids or colonoids.

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Acknowledgments

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & future Planning, Republic of Korea (NRF-2016M3A9D9945475) and by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea (HI16C1634, HI17C2094).

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Authors and Affiliations

  1. Department of Microbiology and Institute of Basic Medical Sciences, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do, 13488, South Korea

    Soojung Hahn & Jongman Yoo

  2. CHA Biocomplex, 335 Pangyo-ro, Seongnam-si, Gyeonggi-do, 13488, South Korea

    Jongman Yoo

Authors
  1. Soojung Hahn
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  2. Jongman Yoo
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Corresponding author

Correspondence to Jongman Yoo .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Hahn, S., Yoo, J. (2017). Gastrointestinal Epithelial Organoid Cultures from Postsurgical Tissues. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY. https://doi.org/10.1007/7651_2017_57

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  • DOI: https://doi.org/10.1007/7651_2017_57

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7616-4

  • Online ISBN: 978-1-4939-7617-1

  • eBook Packages: Springer Protocols

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