Abstract
MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.
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Acknowledgement
This work was supported by a Medical Research Council (grant number G0901155) to FSW and a Diabetes UK PhD studentship to JAP. We acknowledge the NIH Tetramer Core Facility (contract HHSN272201300006C) for provision of MHC | peptide tetramers.
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© 2015 Springer Science+Business Media New York
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Pearson, J.A., Wong, F.S. (2015). Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes. In: Gillespie, K. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 1433. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_295
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DOI: https://doi.org/10.1007/7651_2015_295
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3641-0
Online ISBN: 978-1-4939-3643-4
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