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Decellularization Concept in Regenerative Medicine

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Cell Biology and Translational Medicine, Volume 6

Part of the book series: Advances in Experimental Medicine and Biology ((CBTMED,volume 1212))

Abstract

Decellularized organs and tissues are effectively utilized in a diversity of regenerative medicine purposes, and the decellularization approaches employed differ as broadly as the tissues/organs of concern. Biological scaffold substances formed by extracellular matrix (ECM) are mostly produced with methods that include decellularization of tissues. Conservation of the multifaceted arrangement and three-dimensional (3D) construction of the ECM is very wanted but it is documented that almost every approach of decellularization cause disturbance of the organization and possible forfeiture of surface organization and conformation. The competence of cell elimination from a tissue is reliant on the basis of the tissue and the precise physical, chemical, and enzymatic approaches that are utilized. Here, the most frequently applied and newly developed decellularization techniques are designated, organ engineering with decellularized scaffolds for different organs, recent knowledge in the field are explained.

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Abbreviations

3D:

Three dimensional

ADSCs:

Adipose-derived stem cells

CC10:

Secretoglobin Family 1A

CCSP:

Clara cell secretory protein

CD 31:

Cluster of differentiation 31

CK18:

Keratin 18

ECM:

Extracellular matrix

ESCs:

Embryonic stem cells

FOXJ1:

Forkhead box protein J1

GAG:

Glycosaminoglycans

Mg:

Miligram

MIN-6:

Mouse insulinoma 6

Ng:

Nano gram

Nkx2.1:

NK2 Homeobox 1

PBS:

Phosphate buffered saline

PDGFR:

Platelet derived growth factor receptor

ProSPC:

Alveolar type 2 cell marker

SDS:

Sodium dodecyl sulfate

SPC:

Pulmonary-associated surfactant protein C

TNF:

Tumor necrosis factor

TTF-1:

Transcription termination factor 1

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Authors and Affiliations

  1. Department of Medical Biology, Bahçeşehir University Faculty of Medicine, İstanbul, Turkey

    Özge Sezin Somuncu

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  1. Özge Sezin Somuncu
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Correspondence to Özge Sezin Somuncu .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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Somuncu, Ö.S. (2019). Decellularization Concept in Regenerative Medicine. In: Turksen, K. (eds) Cell Biology and Translational Medicine, Volume 6. Advances in Experimental Medicine and Biology(), vol 1212. Springer, Cham. https://doi.org/10.1007/5584_2019_338

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