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High Prevalence of Human Herpesvirus 8 Infection in Diabetes Type 2 Patients and Detection of a New Virus Subtype

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Advances in Microbiology, Infectious Diseases and Public Health

Part of the book series: Advances in Experimental Medicine and Biology ((AMIDPH,volume 973))

Abstract

The prevalence of Human Herpesvirus 8 (HHV8) DNA and antiviral antibodies in Diabetes type 2 (DM2) and control subjects was studied, in order to confirm a possible link between DM2 and HHV8 infection. The HHV8-DNA from diabetic patients was typed for detecting possible genomic differences with known HHV8 reference viruses.

DM2 patients and healthy controls were examined for the presence of HHV8 DNA into the peripheral blood lymphocytes. Both anti-lytic and latent phase antibodies were detected in HHV8 positive and negative diabetic patients, as well in a number of controls. The HHV8 ORF K1 and ORF 26 genes from DM2 patients were typed and matched to reference strains.

A significant prevalence of HHV8 DNA in DM2 subjects versus healthy controls was detected (about 58 % against 27 %). Anti-lytic phase, but not anti-latent phase antibodies, were significantly increased in DM2 patients versus controls. In addition, about 30 % of HHV8 strains isolated from DM2 lymphocytes showed consistent differences in the ORF 26 gene sequence, so that a new HHV8 subtype was proposed. These findings give additional support to the hypothesis that HHV8 could be considered an additional risk factor for DM2 onset.

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Acknowledgments

The useful help of Mrs Sally Davies for the preparation of the manuscript is kindly acknowledged. This work was sponsored by the Fondazione Banco di Sardegna [No. 0327/2014-15]. The costs to publish in open access were included in funding.

Conflicts of Interest

The authors declare no conflict of interest. The Sponsor had no role in the project designing, in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication.

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Authors and Affiliations

  1. Dipartimento di Scienze Biomediche, Università di Cagliari, via Porcell 4, 0124, Cagliari, Italy

    Enrica Piras, Maria A. Madeddu, Fabrizio Angius, Raffaello Pompei & Angela Ingianni

  2. Policlinico Universitario, Cittadella di Monserrato, Monserrato, Cagliari, Italy

    Giuseppina Palmieri

  3. Servizio di Diabetologia e Malattie Metaboliche, Ospedale S. Giovanni, Cagliari, Italy

    Pierpaolo Contini

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  1. Enrica Piras
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Correspondence to Raffaello Pompei .

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  1. Microbial Biofilm Laboratory, Fondazione Santa Lucia IRCCS, Rome, Italy

    Gianfranco Donelli

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Piras, E. et al. (2016). High Prevalence of Human Herpesvirus 8 Infection in Diabetes Type 2 Patients and Detection of a New Virus Subtype. In: Donelli, G. (eds) Advances in Microbiology, Infectious Diseases and Public Health. Advances in Experimental Medicine and Biology(), vol 973. Springer, Cham. https://doi.org/10.1007/5584_2016_73

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