Abstract
The fluorescence quantum yield Φ f is a key property that characterizes the ability of a fluorophore to convert absorbed photons into emitted photons under various environmental conditions. Knowledge of it is important for the successful development of fluorometric indication and visualization methods and for the understanding of light-driven processes in the natural sciences. Φ f is a molecule- or material-related parameter and can thus significantly differ from the fraction of absorbed photons that are actually measured as the fluorescence signal of a certain sample, e.g., when the signal is modulated by reabsorption, self-quenching or polarization effects. The determination of Φ f can be performed in absolute measurements or relative to a fluorescent standard material with a known Φ f by optical or calorimetric methods. Here the different procedures and techniques are described and compared, and the suitability of a representative number of dyes that have been proposed as fluorescence reference materials in the past 25 years is discussed. Besides addressing specific issues such as low-temperature measurements and the appropriate choice of standards and measurement conditions, we conclude with recommendations for better standardization and quality management in this area.
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References
Lakowicz JR (2006) Principles of fluorescence spectroscopy, 3rd edn. Springer, Berlin
Valeur B (2001) Molecular fluorescence. Wiley-VCH, Weinheim
Lakowicz JR (ed) (1992–2006) Topics in fluorescence spectroscopy series, vols 1–11. Plenum, New York and Springer, Berlin
Wolfbeis OS (ed) (2001–2008) Springer series on fluorescence, vols 1–4. Springer, Berlin
Mycek MA, Pogue BW (eds) (2003) Handbook of biomedical fluorescence. Marcel Dekker, New York
de Silva AP, Tecilla P (eds) (2005) J Mater Chem 15:2637
Pawley JB (ed) (2006) Handbook of biological confocal microscopy, 3rd edn. Springer, Berlin
Duarte FJ, Hillman LW (eds) (1990) Dye laser principles. Academic Press, New York
Ross H, Noakes JE, Spaulding JD (eds) (1991) Liquid scintillation counting and organic scintillators. Lewis, Chelsea, MI
Balzani V, Piotrowiak P, Rodgers MAJ, Mattay J, Astruc D, Gray HB, Winkler J, Fukuzumi S, Mallouk TE, Haas Y, de Silva AP, Gould I (eds) (2001) Electron transfer in chemistry. Wiley-VCH, Weinheim
De Cola L (ed) (2005) Top Curr Chem 257
May V, Kuhn O (2004) Charge and energy transfer dynamics in molecular systems. Wiley-VCH, Weinheim
Earp AA, Smith GB, Swift PD, Franklin J (2004) Sol Energ 76:655
Birks JB (1970) Photophysics of aromatic molecules. Wiley-Interscience, London
Klessinger M, Michl J (1995) Excited states and photochemistry of organic molecules. Wiley-VCH, Weinheim
Lanzani G (ed) (2005) Photophysics of molecular materials. Wiley-VCH, Weinheim
Birks JB (1976) J Res Natl Bur Stand 80A:389
Vavilov SI (1924) Z Phys 22:266
Parker CA, Rees WT (1960) Analyst 85:587
Melhuish WH (1955) N Z J Sci Technol B 37:142
Testa AC (1969) Fluoresc News 4:1
Demas JN, Crosby GA (1971) J Phys Chem 75:991
Melhuish WH (1972) J Res Natl Bur Stand 76A:547
Crosby GA, Demas JN, Callis JB (1972) J Res Natl Bur Stand 76A:561
Reisfeld R (1972) J Res Natl Bur Stand 76A:613
Velapoldi RA (1972) J Res Natl Bur Stand 76A:641
Chen RF (1972) J Res Natl Bur Stand 76A:593
Demas JN, Blumenthal BH (1976) J Res Natl Bur Stand 80A:409
Bril A, Jagerveenis AWD (1976) J Res Natl Bur Stand 80A:401
Callis JB (1976) J Res Natl Bur Stand 80A:413
Demas JN (1982) In: Mielenz KD (ed) Measurement of photolouminescence, optical radiation measurements, vol 3. Academic Press, New York, p 195
Velapoldi RA (1987) In: Burgess C, Mielenz KD (eds) Advances in standards and methodology in spectrophotometry. Elsevier, Amsterdam, p 175
McKinnon RA (1987) In: Burgess C, Mielenz KD (eds) Advances in standards and methodology in spectrophotometry. Elsevier, Amsterdam, p 195
Grum F (1982) Color Res Appl 7:253
Velapoldi RA, Epstein MS (1989) ACS Symp Ser 383:98
Eaton DF (1988) Pure Appl Chem 60:1107
Eaton DF (1988) J Photochem Photobiol B 2:523
Fery-Forgues S, Lavabre D (1999) J Chem Educ 76:1260
Resch-Genger U, Hoffmann K, Nietfeld W, Engel A, Neukammer J, Nitschke R, Ebert B, Macdonald R (2005) J Fluoresc 15:337
Resch-Genger U (2005) J Fluoresc 15:205
Hastings JW, Riley WH, Massa J (1965) J Biol Chem 240:1473
Lee J (1972) Biochemistry 11:3350
Wallace WL, Bard AJ (1979) J Phys Chem 83:1350
Barnett NW, Francis PS (2005) In: Worsford P, Townshend A, Poole C (eds) Encyclopedia of analytical sciences, vol 1, 2nd edn. Elsevier, Amsterdam, p 506
Barnett NW, Francis PS (2005) In: Worsford P, Townshend A, Poole C (eds) Encyclopedia of analytical sciences, vol 1, 2nd edn. Elsevier, Amsterdam, p 511
Kricka LJ (2005) In: Worsford P, Townshend A, Poole C (eds) Encyclopedia of analytical sciences, vol 1, 2nd edn. Elsevier, Amsterdam, p 277
Richter MM (2004) Chem Rev 104:3003
Kankare J, Kulmala S (2005) In: Worsford P, Townshend A, Poole C (eds) Encyclopedia of analytical sciences, vol 1, 2nd edn. Elsevier, Amsterdam, p 528
Beese W, Zink JI (1984) J Luminesc 29:119
Vass I, Govindjee (1996) Photosynth Res 48:117
Peto A (1996) Radiat Prot Dosim 65:123
McKeever SWS, Chen R (1997) Radiat Meas 27:625
Hilgenfeldt S, Grossmann S, Lohse D (1999) Nature 402:398
Bourhill G, Palsson LO, Samuel IDW, Sage IC, Oswald IDH, Duignan JP (2001) Chem Phys Lett 336:234
Didenko YT, Suslick KS (2002) Nature 418:394
Abrams BL, Holloway PH (2004) Chem Rev 104:5783
Furetta C, Kitis G (2004) J Mater Sci 39:2277
Hall LM, Hunter TF, Stock MG (1976) Chem Phys Lett 44:145
Rockley MG (1977) Chem Phys Lett 50:427
Sonnenschein M, Amirav A, Jortner J (1984) J Phys Chem 88:4214
Ates S, Yildiz A (1983) J Chem Soc Faraday Trans 1 79:2853
Alimarin IP, Durnev VF, Runov VK (1987) Teor Eksp Khim 23:705
Ferreira LFV, Freixo MR, Garcia AR, Wilkinson F (1992) J Chem Soc Faraday Trans 88:15
Haynes DR, Tokmakoff A, George SM (1993) Chem Phys Lett 214:50
Mirenda M, Lagorio MG, Roman ES (2004) Langmuir 20:3690
Brody M, Brody SS (1971) Photochem Photobiol 13:293
Kurian E, Prendergast FG, Rudzki Small J (1997) Biophys J 73:466
Greenham NC, Samuel IDW, Hayes GR, Phillips RT, Kessener Y, Moratti SC, Holmes AB, Friend RH (1995) Chem Phys Lett 241:89
deMello JC, Wittmann HF, Friend RH (1997) Adv Mater 9:230
Mattoussi H, Murata H, Merritt CD, Iizumi Y, Kido J, Kafafi ZH (1999) J Appl Phys 86:2642
Nollau A, Hoffmann M, Floreck K, Fritz T, Leo K (2000) J Appl Phys 87:7802
Nonell S, Marti C, Garcia-Moreno I, Costela A, Sastre R (2001) Appl Phys B 72:355
Palsson LO, Monkman AP (2002) Adv Mater 14:757
Weidner VR, Mavrodineanu R, Eckerle KL (1986) Appl Opt 25:832
Seelert W, Strauss E (1987) J Luminesc 36:355
Torchia GA, Munoz JA, Cusso F, Jaque F, Tocho JO (2001) J Luminesc 92:317
Melhuish WH (1984) Pure Appl Chem 56:231
Braslavsky SE (2007) Pure Appl Chem 79:293
Melhuish WH (1961) J Phys Chem 65:229
Melhuish WH (1964) J Opt Soc Am 54:183
Galanin MD, Kut'enkov AA, Smorchkov VN, Timofeev YP, Chizhikov ZA (1982) Opt Spektrosk 53:683
Weber G, Teale FWJ (1957) Trans Faraday Soc 53:646
Hollandt J, Taubert RD, Seidel J, Resch-Genger U, Gugg-Helminger A, Pfeifer D, Monte C, Pilz W (2005) J Fluoresc 15:301
Fletcher AN (1969) Photochem Photobiol 9:439
Rhys Williams AT, Winfield SA, Miller JN (1983) Analyst 108:1067
Bendig J, Kreysig D, Schöneich R (1979) Z Chem 19:151
Demas JN (1973) Anal Chem 45:922
Cehelnik ED, Mielenz KD, Velapoldi RA (1975) J Res Natl Bur Stand 79A:1
Minato H, Nanjo M, Nayatani Y (1983) Color Res Appl 8:238
Levitus M, Bourdelande JL, Marqués G, Aramendía PF (1999) J Photochem Photobiol A 126:77
Resch-Genger U, Pfeifer D, Monte C, Pilz W, Hoffmann A, Spieles M, Rurack K, Hollandt J, Taubert D, Schönenberger B, Nording P (2005) J Fluoresc 15:315
Credi A, Prodi L (1998) Spectrochim Acta 54A:159
Kim CS, Kong HJ (1997) Color Res Appl 22:275
Eastman JW (1967) Photochem Photobiol 6:55
Cundall RB, Evans GB (1968) J Phys E 1:305
Eisinger J, Flores J (1979) Anal Biochem 94:15
Bendig J, Kreysig D (1979) Z Phys Chem 260:149
Bendig J, Csongar C, Kreysig D (1979) Z Phys Chem 260:312
Benson RC, Kues HA (1977) J Chem Eng Data 22:379
Rusakowicz R, Testa AC (1968) J Phys Chem 72:793
Barrow DA, Lentz BR (1984) Chem Phys Lett 104:163
Eastman JW (1970) Spectrochim Acta A26:1545
Chen RF (1967) Anal Lett 1:35
Fletcher AN (1968) J Phys Chem 72:2742
Kubin RF, Fletcher AN (1982) J Luminesc 27:455
Lutz H-P, Luisi PL (1983) Helv Chim Acta 66:1929
De Jersey J, Jeffers Morley P, Martin RB (1981) Biophys Chem 13:233
Bindhu CV, Harilal SS, Nampoori VPN, Vallabhan CPG (1999) Mod Phys Lett B 13:563
Chekalyuk A, Fadeev V, Georgiev G, Kalkanjiev T, Nickolov Z (1982) Spectrosc Lett 15:355
Sivaprakasam V, Killinger DK (2003) J Opt Soc Am B 20:1980
Shea Rohwer L, Martin JE (2005) J Luminesc 115:77
Porrès L, Holland A, Palsson LO, Monkman AP, Kemp C, Beeby A (2006) J Fluoresc 16:267
Seybold PG, Gouterman M, Callis J (1969) Photochem Photobiol 9:229
Adams MJ, Highfield JG, Kirkbright GF (1977) Anal Chem 49:1850
Grabowski JJ, Bertozzi CR, Jacobsen JR, Jain A, Marzluff EM, Suh AY (1992) Anal Biochem 207:214
Görtz W, Perkampus HH (1983) Fresenius Z Anal Chem 316:180
Rockley MG, Waugh KM (1978) Chem Phys Lett 54:597
Vogel M, Rettig W (1985) Ber Bunsenges Phys Chem 89:962
Rudzki Small J, Hutchings JJ, Small EW (1989) Proc SPIE Int Soc Opt Eng 1054:26
Zhang G, Li Z, Yan J (1985) Kexue Tongbao 30:1158
Zhang G, Li Z, Yan J (1986) Chin Phys Lett 3:9
Sabol JE, Rockley MG (1987) J Photochem Photobiol A 40:245
Fischer M, Georges J (1996) Chem Phys Lett 260:115
Magde D, Brannon JH, Cremers TL, Olmsted J III (1979) J Phys Chem 83:696
Hercules DM, Frankel H (1960) Science 131:1611
Olmsted J III (1979) J Phys Chem 83:2581
Magde D, Wong R, Seybold PG (2002) Photochem Photobiol 75:327
Georges J, Arnaud N, Parise L (1996) Appl Spectrosc 50:1505
Sathy P, Philip R, Nampoori VPN, Vallabhan CPG (1990) Pramana 34:585
Bindhu CV, Harilal SS (2001) Anal Sci 17:141
Bindhu CV, Harilal SS, Varier GK, Issac RC, Nampoori VPN, Vallabhan CPG (1996) J Phys D 29:1074
Degen J, Reinecke K, Schmidtke HH (1992) Chem Phys 162:419
Van Houten J, Watts RJ (1975) J Am Chem Soc 97:3843
Nakamaru K, Nishio K, Nobe H (1979) Sci Rep Hirosaki Univ 26:57
Wang T, Zhang BQ, Pan JL, Gu P, Xu Q, Sun Q (1989) Chin Sci Bull 34:1756
Petukhov VA, Popov MB, Krymova AI (1986) Kvantovaya Elektron 13:777
Hamai S, Hirayama F (1983) J Phys Chem 87:83
Morris JV, Mahaney MA, Huber JR (1976) J Phys Chem 80:969
Meech SR, Phillips D (1983) J Photochem 23:193
Velapoldi RA, Tønnesen HH (2004) J Fluoresc 14:465
Pardo A, Reyman D, Poyato JML, Medina F (1992) J Luminesc 51:269
Ghiggino KP, Skilton PF, Thistlethwaite PJ (1985) J Photochem 31:113
Icil H, Uzun D, Pasaogullari N (1998) Spectrosc Lett 31:667
Deltau G, Kringel U, Peros D, Runde B, Drexhage KH (1989) In: Kirov N, Simova P, Jordanov B (eds) Recent developments in molecular spectroscopy: proceedings of the tenth national conference on molecular spectroscopy with international participation. World Scientific, Hackensack, NJ, p 539
O'Neal JS, Schulman SG (1986) Anal Lett 19:495
Wolfbeis OS, Urbano E (1982) J Heterocycl Chem 19:841
Heinrich G, Schoof S, Güsten H (1974) J Photochem 3:315
Mardelli M, Olmsted J III (1977) J Photochem 7:277
Johansson LB-Å, Karolin J, Langhals H, Reichherzer S, von Füner N, Polborn K (1993) J Chem Soc Faraday Trans 89:49
Langhals H, Karolin J, Johansson LB-Å (1998) J Chem Soc Faraday Trans 94:2919
Icli S, Icil H (1996) Spectrosc Lett 29:1253
Icil H, Arslan E (2001) Spectrosc Lett 34:355
Kalinin S, Speckbacher M, Langhals H, Johansson LB-Å (2001) Phys Chem Chem Phys 3:172
Bulska H (1988) J Luminesc 39:293
Wang L, Gaigalas AK, Abbasi F, Marti GE, Vogt RF, Schwartz A (2002) J Res Natl Bur Stand 107:339
Soper SA, Nutter HL, Keller RA, Davis LM, Shera EB (1993) Photochem Photobiol 57:972
Karstens T, Kobs K (1980) J Phys Chem 84:1871
Soper SA, Mattingly QL (1994) J Am Chem Soc 116:3744
Casalboni M, De Matteis F, Prosposito P, Quatela A, Sarcinelli F (2003) Chem Phys Lett 373:372
Mujumdar RB, Ernst LA, Mujumdar SR, Lewis CJ, Waggoner AS (1993) Bioconjugate Chem 4:105
Ernst LA, Gupta RK, Mujumdar RB, Waggoner AS (1989) Cytometry 10:3
Renschler CL, Harrah LA (1983) Anal Chem 55:798
Hurtubise RJ (1990) Phosphorimetry. VCH, New York, p 104
Meyer C (1971) Low temperature spectroscopy. Elsevier, New York, p 203
Pestemer M (1964) Anleitung zum Messen von Absorptionsspektren im Ultraviolett und Sichtbaren. Thieme, Stuttgart
http://www.springer.com/east/home/laboe?SGWID=5-10113-0-0-0&referer=www.landolt-boernstein.com&SHORTCUT=www.springer.com Accessed 11. February 2008
Bendig J, Siegmund M (1981) Z Phys Chem 262:834
Bendig J (1983) Z Phys Chem 264:807
Hurtubise RJ, Thompson AL, Hubbard SE (2005) Anal Lett 38:1823
Li R, Lim EC (1972) J Chem Phys 57:605
Gilmore EH, Gibson GE, McClure DS (1952) J Chem Phys 20:829
Schulman SG, Threatte RM, Capomacchia AC, Paul WL (1974) J Pharm Sci 63:876
Chen RF (1967) Anal Biochem 19:374
Drexhage KH (1976) J Res Natl Bur Stand 80A:421
Gardecki JA, Maroncelli M (1998) Appl Spectrosc 52:1179
Hebert P, Baldacchino G, Gustavsson T, Mialocq JC (1994) J Photochem Photobiol A 84:45
Pfeifer D, Hoffmann K, Hoffmann A, Monte C, Resch-Genger U (2006) J Fluoresc 16:581
Monte C, Resch-Genger U, Pfeifer D, Taubert DR, Hollandt J (2006) Metrologia 43:S89
Boens N, Qin W, Basaric N, Hofkens J, Ameloot M, Pouget J, Lefevre J-P, Valeur B, Gratton E, vandeVen M, Silva ND Jr, Engelborghs Y, Willaert K, Sillen A, Rumbles G, Phillips D, Visser AJWG, van Hoek A, Lakowicz JR, Malak H, Gryczynski I, Szabo AG, Krajcarski DT, Tamai N, Miura A (2007) Anal Chem 79:2137
Du H, Fuh RCA, Li J, Corkan LA, Lindsey JS (1998) Photochem Photobiol 68:141
Dawson WR, Windsor MW (1968) J Phys Chem 72:3251
Bowen EJ, Sahu J (1959) J Phys Chem 63:4
Berlman IB (1971) Handbook of fluorescence spectra of aromatic molecules. Academic Press, New York
Rusakowicz R, Testa AC (1968) J Phys Chem 72:2680
Velapoldi RA, Mielenz KD (1980) NBS Special Publication No 260-64. US Government Printing Office, Washington, DC
Tsin ATC, Pedrozo-Fernandez HA, Gallas JM, Chambers JP (1988) Life Sci 43:1379
Weber G, Teale FWJ (1958) Trans Faraday Soc 54:640
Murov SL, Carmichael I, Hug GL (1993) Handbook of photochemistry, 2nd edn. Marcel Dekker, New York
Drexhage KH (1990) In: Schäfer FP (ed) Dye lasers, 3rd edn. Springer, Berlin, p 155
Drexhage KH (1997) NBS Special Publication 466, Paper 80A3-894. Washington, DC
Roth NJL, Craig AC (1974) J Phys Chem 78:1154
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Rurack, K. (2008). Fluorescence Quantum Yields: Methods of Determination and Standards. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements I. Springer Series on Fluorescence, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_019
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