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Genetics and classical genetic manipulations of industrial yeasts

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Functional Genetics of Industrial Yeasts

Part of the book series: Topics in Current Genetics ((TCG,volume 2))

Abstract

Yeasts represent highly important and valuable organisms for research and industrial applications. Although Saccharomyces cerevisiae has historically been a key species, other yeast genera and species are increasingly important. The rapid emergence and expansion of genomic and functional genetic information on various yeasts indicates a high level of complexity amongst these organisms. It is apparent that the S. cerevisiae genome was duplicated some 100 million years ago and that several of the other important yeast species diverged prior to this duplication, whilst others diverged post-polyploidization of the ancestral yeast genome. Here we present an overview of the various yeast genomes that serves to highlight the complexity of yeasts, especially within the context of industrially relevant strains. The challenges faced by industrial yeast geneticists are discussed and key issues concerning strategies of mating, mutagenesis, spheroplast fusion and cytoduction are presented. Examples of strain improvements achieved via classical genetics are presented, along with an analysis of molecular genetics of maltose metabolism in industrial yeasts, which serves to highlight the usefulness of combined molecular and classical genetics in strain improvement.

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Authors
  1. Paul V. Attfield
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  2. Philip J. L. Bell
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  1. Beyerinck Laboratory DSM Life Sciences, Bakery Ingredients Technology, PO Box 1, 2600 MA, Delft, The Netherlands

    Johannes H. de Winde

  2. Kluyver Laboratory for Biotechnology, Technical University of Delft, Julianalaan 67, 2628 BC, Delft, The Netherlands

    Johannes H. de Winde

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Attfield, P.V., Bell, P.J.L. (2003). Genetics and classical genetic manipulations of industrial yeasts. In: de Winde, J.H. (eds) Functional Genetics of Industrial Yeasts. Topics in Current Genetics, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-37003-X_2

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  • DOI: https://doi.org/10.1007/3-540-37003-X_2

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