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Protein Inclusion Bodies in Recombinant Bacteria

  • Chapter
Inclusions in Prokaryotes

Part of the book series: Microbiology Monographs ((MICROMONO,volume 1))

Abstract

Fast and high-level expression of heterologous proteins in bacterial hosts often results in the accumulation of almost pure aggregates (inclusion bodies, IBs) of the target protein. Although knowledge of the pathways and influential factors of protein folding in vivo has increased for many years, the complexity of the cellular networks does not allow easily the prediction of favourable conditions for production of correctly folded proteins. Therefore, IB-based production is still a potential and straightforward strategy for the production of complex recombinant proteins. IB-based processes combine the advantages of a high concentration of the target protein produced in well-characterized bacteria such as Escherichia coli, efficient protocols for IB isolation, purification and in vitro protein refolding without the need of elaborate coexpression systems and time-consuming trial-and-error expression optimization. Recent advances in understanding the molecular physiology of IB formation and in resolubilization enable a streamlined development of fermentation processes to obtain a high-quality product. In addition, simple strategies have been established to improve the purification and renaturation of disulfide bond containing proteins allowing for a fast transfer of those processes to industrial production scale.

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Authors and Affiliations

  1. Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, PO Box 4300, 90014, Oulu, Finland

    Peter Neubauer

  2. Cardiff School of Biosciences, Biomedical Sciences Building (BIOSI2), Cardiff University, PO Box 911, Cardiff, CF10 3US, UK

    Beatrix Fahnert

  3. Institute of Biotechnology, Martin Luther University Halle-Wittenberg, 06099, Halle (Saale), Germany

    Hauke Lilie

  4. Institut de Biotecnologia i de Biomedicina and Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain

    Antonio Villaverde

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  1. Peter Neubauer
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  2. Beatrix Fahnert
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  4. Antonio Villaverde
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  1. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634, USA

    Jessup M. Shively (Prof. Emeritus of Biochemistry) (Prof. Emeritus of Biochemistry)

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Neubauer, P., Fahnert, B., Lilie, H., Villaverde, A. (2006). Protein Inclusion Bodies in Recombinant Bacteria. In: Shively, J.M. (eds) Inclusions in Prokaryotes. Microbiology Monographs, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-33774-1_9

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