Synonyms
DNA and RNA blotting, nucleic acid blotting.
The southern and northern blotting techniques. Enzymatically digested DNA or denatured RNA is fractionated by gel electrophoresis. After transfer and fixation of the DNA fragments or RNA to a membrane support, the membrane is incubated with a labeled DNA or RNA probe that is specific for the target of interest. Because the probe hybridizes to only the target fragment/molecule, a specific band is visualized following detection.
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References
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Alwine JC, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74:5350–5354
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© 2005 Springer-Verlag
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Trouba, K. (2005). Southern and Northern Blotting. In: Vohr, HW. (eds) Encyclopedic Reference of Immunotoxicology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-27806-0_1368
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DOI: https://doi.org/10.1007/3-540-27806-0_1368
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-44172-4
Online ISBN: 978-3-540-27806-1
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