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Immobilization of Oligonucleotides for Biochemical Sensing by Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces

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Immobilisation of DNA on Chips I

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 260))

Abstract

The covalent immobilization of biomolecules on surfaces is of great interest for many medical and bioanalytical applications. Currently, there is a wide range of procedures available for the modification of surfaces to achieve covalent immobilization. Self-assembled monolayers (SAMs) are easy to generate, offer a high level of flexibility and can be precisely modified on a molecular level. Therefore, the use of SAMs for various fields in research and commercial applications is increasing fast. One of the most interesting applications is the utilization of such layers for the attachment of biomolecules. The emphasis of this review is the discussion of different aspects of uniform, mixed, and functionalized monolayers regarding their use for the immobilization of biological recognition molecules such as oligonucleotides (DNA, RNA) in comparison to proteins (especially antibodies and receptor molecules) for bioanalytical applications. In doing so, we focus on the generation of self-organizing monolayers either on smooth gold surfaces by attachment of thiol compounds or on silica surfaces by silanization.

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Abbreviations

Å:

angstrom (metric unit)---Ten billion angstroms equal 1 meter

APTMS:

3-aminopropyltrimethoxysilane

APTES:

3-aminopropyltriethoxysilane

AFM:

atomic force microscopy

ELISA:

enzyme-linked immunosorbent assay

FMOC:

9-fluorenylmethoxycarbonyl

FT-IR:

Fourier transform infrared spectroscopy

GMBS:

N-(γ-maleimidobutyryloxy)succinimide ester

HSAB:

N-hydroxysuccinimidyl-4-azidobenzoic acid

NHS:

N-hydroxysuccinimide ester

PEG:

poly(ethylene glycol)

QCM:

quartz crystal microbalance

SAM:

self-assembled monolayer

SCE:

saturated calomel electrode

SNOM:

scanning near field optical microscopy

SPM:

scanning probe microscopy

SPR:

surface plasmon resonance

STM:

scanning tunneling microscopy

Sulfo-SMCC:

sulfosuccinimidyl 4-N-maleimidomethyl cyclohexane-1-carboxylate

THF:

tetrahydrofuran

TMOS:

tetramethylorthosilicate

XPS:

X-ray photoelectron spectroscopy

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Authors and Affiliations

  1. Neubrandenburg University of Applied Sciences, Department of Technology, Fachhochschule Neubrandenburg, Brodaer Strasse 2, D-17033, Neubrandenburg, Germany

    F. Luderer & U. Walschus

Authors
  1. F. Luderer
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  2. U. Walschus
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Correspondence to F. Luderer .

Editor information

Christine Wittmann

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Luderer, F., Walschus, U. Immobilization of Oligonucleotides for Biochemical Sensing by Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces. In: Wittmann, C. (eds) Immobilisation of DNA on Chips I. Topics in Current Chemistry, vol 260. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_003

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