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Adaptive Feature Selection for Classification of Microscope Images

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Fuzzy Logic and Applications (WILF 2005)

Part of the book series: Lecture Notes in Computer Science ((LNAI,volume 3849))

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Abstract

For high-throughput screening of genetically modified plant cells, a system for the automatic analysis of huge collections of microscope images is needed to decide whether the cells are infected with fungi or not. To study the potential of feature based classification for this application, we compare different classifiers (kNN, SVM, MLP, LVQ) combined with several feature reduction techniques (PCA, LDA, Mutual Information, Fisher Discriminant Ratio, Recursive Feature Elimination). We achieve a significantly higher classification accuracy using a reduced feature vector instead of the full length feature vector.

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References

  1. Bengtsson, E.: Computerized cell image analysis: Past, present, and future. In: Bigun, J., Gustavsson, T. (eds.) SCIA 2003. LNCS, vol. 2749, pp. 395–407. Springer, Heidelberg (2003)

    Chapter  Google Scholar 

  2. Cover, T.M., Thomas, J.A.: Elements of Information Theory. Wiley, New York (1991)

    Book  MATH  Google Scholar 

  3. da Costa, L.F., Cesar Jr., R.M.: Shape Analysis and Classification. CRC Press, Boca Raton (2001)

    MATH  Google Scholar 

  4. Duchene, J., Leclercq, S.: An optimal transformation for discriminant and principal component analysis. IEEE Transactions on Pattern Analysis and Machine Intelligence (PAMI) 10(6), 978–983 (1988)

    Article  MATH  Google Scholar 

  5. Gonzalez, R.C., Woods, R.E.: Digital Image Processing. Addison-Wesley, Reading (1993)

    Google Scholar 

  6. Granlund, G.H.: Fourier preprocessing for hand print character recognition. IEEE Transactions on Computers C-21(3), 195–201 (1972)

    Article  MathSciNet  Google Scholar 

  7. Guyon, I., Weston, J., Barnhill, S., Vapnik, V.: Gene selection for cancer classification using support vector machines. Machine Learning 46(1-3), 389–422 (2002)

    Article  MATH  Google Scholar 

  8. Hu, M.-K.: Visual pattern recognition by moment invariants. IRE Transactions on Information Theory 8(2), 179–187 (1962)

    Article  Google Scholar 

  9. Ihlow, A., Seiffert, U.: Microscope color image segmentation for resistance analysis of barley cells against powdery mildew. In: 9. Workshop Farbbildverarbeitung, ZBS Zentrum für Bild- und Signalverarbeitung e.V. Ilmenau, Report Nr. 3/2003, pp. 59–66, Ostfildern-Nellingen, Germany (October 2003)

    Google Scholar 

  10. Ihlow, A., Seiffert, U.: Automating microscope colour image analysis using the Expectation Maximisation algorithm. In: Rasmussen, C.E., Bülthoff, H.H., Schölkopf, B., Giese, M.A. (eds.) DAGM 2004. LNCS, vol. 3175, pp. 536–543. Springer, Heidelberg (2004)

    Chapter  Google Scholar 

  11. Ihlow, A., Seiffert, U.: Haustoria segmentation in microscope images of barley cells. In: Workshop ‘Farbbildverarbeitung’, Koblenz, October 2004, vol. 10, pp. 119–126. Der andere Verlag (2004)

    Google Scholar 

  12. Mokhtarian, F., Mackworth, A.: Scale-based description and recognition of planar curves and two-dimensional shapes. IEEE Transactions on Pattern Analysis and Machine Intelligence (PAMI) 8(1), 34–43 (1986)

    Article  Google Scholar 

  13. Nadeau, C., Bengio, Y.: Inference for the generalization error. In: Advances in Neural Information Processing Systems, vol. 12. MIT Press, Cambridge (2000)

    Google Scholar 

  14. Schweizer, P., Pokorny, J., Abderhalden, O., Dudler, R.: A transient assay system for the functional assessment of defense-related genes in wheat. Molecular Plant-Microbe Interactions 12(8), 647–654 (1999)

    Article  Google Scholar 

  15. Street, W.N., Wolberg, W.H., Mangasarian, O.L.: Nuclear feature extraction for breast tumor diagnosis. Biomedical Image Processing and Biomedical Visualization 1905, 861–870 (1993)

    Google Scholar 

  16. Tang, X., Stewart, W.K., Vincent, L., Huang, H., Marra, M., Gallager, S., Davis, C.S.: Automatic plankton image recognition. Artificial Intelligence Review 12(1-3), 177–199 (1998)

    Article  MATH  Google Scholar 

  17. Theodordis, S., Koutroumbas, K.: Pattern Recognition. Elsevier Academic Press, San Diego (2003)

    Google Scholar 

  18. Thiran, J.-P., Macq, B.M.: Morphological feature extraction for the classification of digital images of cancerous tissues. IEEE Transactions on Biomedical Engineering 43(10), 1011–1020 (1996)

    Article  Google Scholar 

  19. Weston, J., Elisseeff, A., BakIr, G., Sinz, F.: The SPIDER: object-orientated machine learning library, http://www.kyb.tuebingen.mpg.de/bs/people/spider/index.html

  20. Wolberg, W.H., Street, W.N., Mangasarian, O.L.: Breast cytology diagnosis via digital image analysis. Analytical and Quantitative Cytology and Histology 15(6), 396–404 (1993)

    Google Scholar 

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© 2006 Springer-Verlag Berlin Heidelberg

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Tautenhahn, R., Ihlow, A., Seiffert, U. (2006). Adaptive Feature Selection for Classification of Microscope Images. In: Bloch, I., Petrosino, A., Tettamanzi, A.G.B. (eds) Fuzzy Logic and Applications. WILF 2005. Lecture Notes in Computer Science(), vol 3849. Springer, Berlin, Heidelberg. https://doi.org/10.1007/11676935_26

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  • DOI: https://doi.org/10.1007/11676935_26

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-32529-1

  • Online ISBN: 978-3-540-32530-7

  • eBook Packages: Computer ScienceComputer Science (R0)

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