Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- APO:
-
2-aminophenoxazin-3-one
- NADH:
-
Reduced form of nicotinamide adenine dinucleotide
- NADPH:
-
Reduced form of nicotinamide adenine dinucleotide phosphate
- Ni–NTA:
-
Nickel-nitrilotriacetic acid
- LbL:
-
Layer-by-layer
- RAMA:
-
Fructose-1,6-bisphosphate aldolase from rabbit muscle
- PDMS:
-
Polydimethylsiloxane
- LTCC:
-
Low temperature co-fired ceramics
- CIM® :
-
Convective Interaction Media
- GA:
-
Glutaraldehyde
- PTFE:
-
Poly(tetrafluoroethene)
- PS:
-
Polystirene
- PB:
-
Prussian Blue
- THN:
-
1,3,6,8-tetrahydroxynaphthalene
- Au-MSNP:
-
Gold-magnetic silica nanoparticles
- GOD:
-
Glucose oxidase
- POD:
-
Horseradish peroxidase
- BSA:
-
Bovine serum albumin
- FateDH:
-
Formate dehydrogenase
- FaldDH:
-
Formaldehyde dehydrogenase
- ADH:
-
Alcohol dehydrogenase
References
Cornish-Bowden A (2004) Fundamentals of enzyme kinetics. Portland Press, London
Hodgman CE, Jewett MC (2012) Cell-free synthetic biology: thinking outside the cell. Metab Eng 14:261–269
Lopez-Gallego F, Schmidt-Dannert C (2010) Multi-enzymatic synthesis. Curr Opin Chem Biol 14:174–183
Findrik Z, Vasić-Rački Đ (2009) Overview on reactions with multi-enzyme systems. Chem Biochem Eng Q 23:545–553
Santacoloma PA, Sin G, Gernaey KV, Woodley JM (2010) Multienzyme-catalyzed processes: next-generation biocatalysis. Org Process Res Dev 15:203–212
Ricca E, Brucher B, Schrittwieser JH (2011) Multi-Enzymatic cascade reactions: overview and perspectives. Adv Synth Catal 353:2239–2262
Asanomi Y, Yamaguchi H, Miyazaki M, Maeda H (2011) Enzyme-immobilized microfluidic process reactors. Molecules 16:6041–6059
Logan TC, Clark DS, Stachowiak TB, Svec F, Fréchet JMJ (2007) Photopatterning enzymes on polymer monoliths in microfluidic devices for steady-state kinetic analysis and spatially separated multi-enzyme reactions. Anal Chem 79:6592–6598
Andrianantoandro E, Basu S, Karig DK, Weiss R (2006) Synthetic biology: new engineering rules for an emerging discipline. Mol Syst Biol 2(2006):0028
Forster AC, Church GM (2006) Synthetic biology projects in vitro. Genome Res 17:000
Keasling JD (2008) Synthetic biology for synthetic chemistry. ACS Chem Biol 3:64–76
Chenault HK, Simon ES, Whitesides GM (1988) Cofactor regeneration for enzyme-catalysed synthesis. Biotechnol Genet Eng Rev 6:221–270
Lauterbach L, Lenz O, Vincent KA (2013) H2-driven cofactor regeneration with NAD(P)+-reducing hydrogenases. FEBS J, n/a-n/a.
Liu W, Wang P (2007) Cofactor regeneration for sustainable enzymatic biosynthesis. Biotechnol Adv 25:369–384
Wichmann R, Vasic-Racki D (2005) Cofactor regeneration at the lab scale. Springer, Berlin
Xue R, Woodley JM (2012) Process technology for multi-enzymatic reaction systems. Bioresour Technol 115:183–195
Zhao H, van der Donk WA (2003) Regeneration of cofactors for use in biocatalysis. Curr Opin Biotechnol 14:583–589
Findrik Z, Vasić-Rački Đ (2008) Mathematical modelling of amino acid resolution catalyzed by l-amino acid oxidases from Crotalus adamanteus and Crotalus atrox. Process Biochem 43:1186–1194
Plumeré N, Henig J, Campbell WH (2012) Enzyme-catalyzed O2 removal system for electrochemical analysis under ambient air: Application in an amperometric nitrate biosensor. Anal Chem 84:2141–2146
Srere PA, Mattiasson B, Mosbach K (1973) An immobilized three-enzyme system: a model for microenvironmental compartmentation in mitochondria. P Natl Acad Sci 70:2534–2538
Yun H, Yang Y-H, Cho B-K, Hwang B-Y, Kim B-G (2003) Simultaneous synthesis of enantiomerically pure (R)-1-phenylethanol and (R)-α-methylbenzylamine from racemic α-methylbenzylamine using ω-transaminase/alcohol dehydrogenase/glucose dehydrogenase coupling reaction. Biotechnol Lett 25:809–814
Soldatkin OO, Peshkova VM, Dzyadevych SV, Soldatkin AP, Jaffrezic-Renault N, El’skaya AV (2008) Novel sucrose three-enzyme conductometric biosensor. Mat Sci Eng C 28:959–964
Ishii N, Suga Y, Hagiya A, Watanabe H, Mori H, Yoshino M, Tomita M (2007) Dynamic simulation of an in vitro multi-enzyme system. FEBS Lett 581:413–420
Bae H-S, Lee S-G, Hong S-P, Kwak M-S, Esaki N, Soda K, Sung M-H (1999) Production of aromatic D-amino acids from α-keto acids and ammonia by coupling of four enzyme reactions. J Mol Catal B Enzym 6:241–247
Findrik Z, Vasić-Rački Đ (2007) Biotransformation of d-methionine into l-methionine in the cascade of four enzymes. Biotechnol Bioeng 98:956–967
Gijsen HJM, Qiao L, Fitz W, Wong C-H (1996) Recent advances in the chemoenzymatic synthesis of carbohydrates and carbohydrate mimetics. Chem Rev 96:443–474
Roessner CA, Scott AI (1996) Achieving natural product synthesis and diversity via catalytic networking ex vivo. Chem Biol 3:325–330
Zhang Y-HP (2010) Production of biocommodities and bioelectricity by cell-free synthetic enzymatic pathway biotransformations: challenges and opportunities. Biotechnol Bioeng 105:663–677
Fessner W-D, Walter C (1992) “Artificial metabolisms” for the asymmetric one-pot synthesis of branched-chain saccharides. Angew Chem Int Edit 31:614–616
Itoh A, Ohashi Y, Soga T, Mori H, Nishioka T, Tomita M (2004) Application of capillary electrophoresis-mass spectrometry to synthetic in vitro glycolysis studies. Electrophoresis 25:1996–2002
Wendell D, Todd J, Montemagno C (2010) Artificial photosynthesis in Ranaspumin-2 Based Foam. Nano Lett 10:3231–3236
Zhang YHP (2009) Using extremophile enzymes to generate hydrogen for electricity. Microbe 4:560–565
Woodward J, Orr M, Cordray K, Greenbaum E (2000) Biotechnology: enzymatic production of biohydrogen. Nature 405:1014–1015
Zhang YHP, Evans BR, Mielenz JR, Hopkins RC, Adams MWW (2007) High-yield hydrogen production from starch and water by a synthetic enzymatic pathway. PLoS ONE 2:e456
Zhang YHP, Huang W-D (2012) Constructing the electricity–carbohydrate–hydrogen cycle for a sustainability revolution. Trends Biotechnol 30:301–306
Wang Y, Huang W, Sathitsuksanoh N, Zhu Z, Zhang YHP (2011) Biohydrogenation from biomass sugar mediated by in vitro synthetic enzymatic pathways. Chem Biol 18:372–380
Ardao I, Zeng A-P (2013) In silico evaluation of a complex multi-enzymatic system using one-pot and modular approaches: application to the high-yield production of hydrogen from a synthetic metabolic pathway. Chem Eng Sci 87:183–193
Bouzas TdM, Barros-Velázquez J, González Villa T (2006) Industrial applications of hyperthermophilic enzymes: a review. Protein Pept Lett 13:645–651
Myung S, Wang Y, Zhang YHP (2010) Fructose-1,6-bisphosphatase from a hyper-thermophilic bacterium Thermotoga maritima: characterization, metabolite stability, and its implications. Process Biochem 45:1882–1887
Chen AH, Silver PA (2012) Designing biological compartmentalization. Trends Cell Biol 22:662–670
Jandt U, You C, Zhang YHP, Zeng AP (2013) Compartmentalization and metabolic channeling for multienzymatic biosynthesis: practical strategies and modeling approaches. Adv Biochem Eng/Biotechnol (accepted)
Monti D, Ferrandi EE, Zanellato I, Hua L, Polentini F, Carrea G, Riva S (2009) One-pot mutienzymatic synthesis of 12-ketoursodeoxycholic acid: Subtle cofactor specificities rule the reaction equilibria of five biocatalysts working in a row. Adv Synth Catal 351:1303–1311
Babich L (2013) Enzymatic cascade reactions involving phosphorylated intermediates: immobilization and process optimization. Faculty of Science, University of Amsterdam, Amsterdam (The Netherlands)
Zhang X, Stefanick S, Villani FJ (2004) Application of microreactor technology in process development. Org Process Res Dev 8:455–460
Urban PL, Goodall DM, Bruce NC (2007) Enzymatic microreactors in chemical analysis and kinetic studies. Biotechnol Adv 24:42–57
Miyazaki M, Maeda H (2006) Microchannel enzyme reactors and their applications for processing. Trends Biotechnol 24:463–470
Matosevic S, Szita N, Baganz F (2011) Fundamentals and applications of immobilized microfluidic enzymatic reactors. J Chem Technol Biotechnol 86:325–334
Ehrfeld W, Hessel V, Löwe H (2000) Microreactors. Wiley-VCH, Weinheim
Rajabi N, Hoffmann M, Bahnemann J, Zeng A-P, Schl, uuml, ter M, ller J, ouml rg (2012) A Chaotic Advection Enhanced Microfluidic Split-and-Recombine Mixer for the Preparation of Chemical and Biological Probes. J Chem Eng Jpn 45:703–707
Tokeshi M, Minagawa T, Uchiyama K, Hibara A, Sato K, Hisamoto H, Kitamori T (2002) Continuous-flow chemical processing on a microchip by combining microunit operations and a multiphase flow network. Anal Chem 74:1565–1571
Hibara A, Nonaka M, Hisamoto H, Uchiyama K, Kikutani Y, Tokeshi M, Kitamori T (2002) Stabilization of liquid interface and control of two-phase confluence and separation in glass microchips by utilizing octadecylsilane modification of microchannels. Anal Chem 74:1724–1728
Yamaguchi Y, Ogino K, Takagi F, Honda T, Yamashita K, Miyazaki M, Nakamura H, Maeda H (2005) Partial chemical modification of a microchannel and stabilization of water-oil phase separation, Proceedings of the 8th International Conference on Microreaction Technology (IMRET 8). American Institute of Chemical Engineers, New York
Zhao B, Moore JS, Beebe DJ (2002) Principles of surface-directed liquid flow in microfluidic channels. Anal Chem 74:4259–4268
Erickson D, Li D (2002) Influence of surface heterogeneity on electrokinetically driven microfluidic mixing. Langmuir 18:1883–1892
Seong GH, Crooks RM (2002) Efficient mixing and reactions within microfluidic channels using microbead-supported catalysts. J Am Chem Soc 124:13360–13361
Stroock AD, Dertinger SKW, Ajdari A, Mezić I, Stone HA, Whitesides GM (2002) Chaotic mixer for microchannels. Science 295:647–651
Kim J-H, Kin B-G, La M, Yoon J-B, Yoon E (2002) A dispossable passive microfluidic system integrated with micromixer and DNA purification chip for DNA sample preparation. Micro total analysis sytem. Dordrecht, The Netherlands, pp 224–226
Mengeaud V, Josserand J, Girault HH (2002) Mixing processes in a zigzag microchannel: finite element simulations and optical study. Anal Chem 74:4279–4286
Lee M-Y, Srinivasan A, Ku B, Dordick JS (2003) Multienzyme catalysis in microfluidic biochips. Biotechnol Bioeng 83:20–28
Richter T, Shultz-Lockyear LL, Oleschuk RD, Bilitewski U, Harrison DJ (2002) Bi-enzymatic and capillary electrophoretic analysis of non-fluorescent compounds in microfluidic devices: determination of xanthine. Sens Actuators B: Chem 81:369–376
Srinivasan A, Bach H, Sherman DH, Dordick JS (2004) Bacterial P450-catalyzed polyketide hydroxylation on a microfluidic platform. Biotechnol Bioeng 88:528–535
Drager G, Kiss C, Kunz U, Kirschning A (2007) Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix. Org Biomol Chem 5:3657–3664
Sakai-Kato K, Kato M, Ishihara K, Toyo’oka T (2004) An enzyme-immobilization method for integration of biofunctions on a microchip using a water-soluble amphiphilic phospholipid polymer having a reacting group. Lab Chip 4:4–6
Heule M, Rezwan K, Cavalli L, Gauckler LJ (2003) A miniaturized enzyme reactor based on hierarchically shaped porous ceramic microstruts. Adv Mater 15:1191–1194
Krenkova J, Lacher NA, Svec F (2009) Highly efficient enzyme reactors containing trypsin and endoproteinase Lysc immobilized on porous polymer monolith coupled to ms suitable for analysis of antibodies. Anal Chem 81:2004–2012
Delattre C, Vijayalakshmi MA (2009) Monolith enzymatic microreactor at the frontier of glycomic toward a new route for the production of bioactive oligosaccharides. J Mol Catal B Enzym 60:97–105
Yang C, Zhang Z, Shi Z, Xue P, Chang P, Yan R (2010) Development of a novel enzyme reactor and application as a chemiluminescence flow-through biosensor. Anal Bioanal Chem 397:2997–3003
Baeza M, López C, Alonso Jn, López-Santín J, Álvaro G (2009) Ceramic microsystem incorporating a microreactor with immobilized biocatalyst for enzymatic spectrophotometric assays. Anal Chem 82:1006–1011
Gleason NJ, Carbeck JD (2004) Measurement of enzyme kinetics using microscale steady-state kinetic analysis. Langmuir 20:6374–6381
Mao H, Yang T, Cremer PS (2001) Design and characterization of immobilized enzymes in microfluidic systems. Anal Chem 74:379–385
Holden MA, Jung S-Y, Cremer PS (2004) Patterning enzymes inside microfluidic channels via photoattachment chemistry. Anal Chem 76:1838–1843
Ekström S, Önnerfjord P, Nilsson J, Bengtsson M, Laurell T, Marko-Varga G (1999) Integrated microanalytical technology enabling rapid and automated protein identification. Anal Chem 72:286–293
Qu H, Wang H, Huang Y, Zhong W, Lu H, Kong J, Yang P, Liu B (2004) Stable microstructured network for protein patterning on a plastic microfluidic channel: strategy and characterization of on-chip enzyme microreactors. Anal Chem 76:6426–6433
Wu H, Tian Y, Liu B, Lu H, Wang X, Zhai J, Jin H, Yang P, Xu Y, Wang H (2004) Titania and alumina sol − gel-derived microfluidics enzymatic-reactors for peptide mapping: design, characterization, and performance. J Proteome Res 3:1201–1209
Ji, Zhang Y, Zhou X, Kong J, Tang Y, Liu B (2008) Enhanced protein digestion through the confinement of nanozeolite-assembled microchip reactors. Anal Chem 80:2457–2463
Thomsen MS, Polt P, Nidetzky B (2007) Development of a microfluidic immobilised enzyme reactor. Chemical Communi 0:2527–2529
Nakamura H, Li X, Wang H, Uehara M, Miyazaki M, Shimizu H, Maeda H (2004) A simple method of self assembled nano-particles deposition on the micro-capillary inner walls and the reactor application for photo-catalytic and enzyme reactions. Chem Eng J 101:261–268
Schilke KF, Wilson KL, Cantrell T, Corti G, McIlroy DN, Kelly C (2010) A novel enzymatic microreactor with Aspergillus oryzae β-galactosidase immobilized on silicon dioxide nanosprings. Biotechnol Prog 26:1597–1605
Koh W-G, Pishko M (2005) Immobilization of multi-enzyme microreactors inside microfluidic devices. Sens Actuators B: Chem 106:335–342
Gao J, Xu J, Locascio LE, Lee CS (2001) Integrated microfluidic system enabling protein digestion, peptide separation, and protein identification. Anal Chem 73:2648–2655
Hisamoto H, Shimizu Y, Uchiyama K, Tokeshi M, Kikutani Y, Hibara A, Kitamori T (2002) Chemicofunctional membrane for integrated chemical processes on a microchip. Anal Chem 75:350–354
Ku B, Cha J, Srinivasan A, Kwon SJ (2006) Chip-based polyketide biosynthesis and functionalization. Biotechnol Prog 22:1102–1107
Matosevic S, Lye GJ, Baganz F (2011) Immobilised enzyme microreactor for screening of multi-step bioconversions: Characterisation of a de novo transketolase-ω-transaminase pathway to synthesise chiral amino alcohols. J Biotechnol 155:320–329
Lee S-H, Lee C-S, Kim B-G, Kim Y-K (2008) An integrated microfluidic chip for the analysis of biochemical reactions by MALDI mass spectrometry. Biomed Microdevices 10:1–9
Hwang ET, Gu MB (2013) Enzyme stabilization by nano/microsized hybrid materials. Eng Life Sci 13:49–61
Bäumler H, Georgieva R (2010) Coupled enzyme reactions in multicompartment microparticles. Biomacromolecules 11:1480–1487
Caruso F, Schüler C (2000) Enzyme multilayers on colloid particles: assembly, stability, and enzymatic activity. Langmuir 16:9595–9603
Cho EJ, Jung S, Kim HJ, Lee YG, Nam KC, Lee H-J, Bae H-J (2012) Co-immobilization of three cellulases on Au-doped magnetic silica nanoparticles for the degradation of cellulose. Chem Commun 48:886–888
Jia F, Zhang Y, Narasimhan B, Mallapragada SK (2012) Block copolymer-quantum dot micelles for multienzyme colocalization. Langmuir 28:17389–17395
Niu H, Yuan R, Chai Y, Mao L, Liu H, Cao Y (2013) Highly amplified electrochemiluminescence of peroxydisulfate using bienzyme functionalized palladium nanoparticles as labels for ultrasensitive immunoassay. Biosens Bioelectron 39:296–299
Pescador P, Katakis I, Toca-Herrera JL, Donath E (2008) Efficiency of a bienzyme sequential reaction system immobilized on polyelectrolyte multilayer-coated colloids. Langmuir 24:14108–14114
Zhuo Y, Yuan P-X, Yuan R, Chai Y-Q, Hong C-L (2009) Bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors. Biomaterials 30:2284–2290
Betancor L, Berne C, Luckarift HR, Spain JC (2006) Coimmobilization of a redox enzyme and a cofactor regeneration system. Chem Commun 0:3640–3642
Delaittre G, Reynhout IC, Cornelissen JJLM, Nolte RJM (2009) Cascade reactions in an all-enzyme nanoreactor. Chem A Eur J 15:12600–12603
Kreft O, Prevot M, Möhwald H, Sukhorukov GB (2007) Shell-in-shell microcapsules: a novel tool for integrated, spatially confined enzymatic reactions. Angew Chem Int Ed 46:5605–5608
Meeuwissen SA, Rioz-Martinez A, de Gonzalo G, Fraaije MW, Gotor V, van Hest JCM (2011) Cofactor regeneration in polymersome nanoreactors: enzymatically catalysed baeyer-villiger reactions. J Mater Chem 21:18923–18926
Tanner P, Onaca O, Balasubramanian V, Meier W, Palivan CG (2011) Enzymatic cascade reactions inside polymeric nanocontainers: a means to combat oxidative stress. Chem A Eur J 17:4552–4560
van Dongen SFM, Nallani M, Cornelissen JJLM, Nolte RJM, Van Hest JCM (2009) A three-enzyme cascade reaction through positional assembly of enzymes in a polymersome nanoreactor. Chem A Eur J 15:1107–1114
Zhang L, Shi J, Jiang Z, Jiang Y, Qiao S, Li J, Wang R, Meng R, Zhu Y, Zheng Y (2011) Bioinspired preparation of polydopamine microcapsule for multienzyme system construction. Green Chem 13:300–306
Schoffelen S, van Hest JCM (2012) Multi-enzyme systems: bringing enzymes together in vitro. Soft Matter 8:1736–1746
Vriezema DM, Garcia PML, Sancho|Oltra N, Hatzakis NS, Kuiper SM, Nolte RJM, Rowan AE, Van|Hest JCM (2007) Positional assembly of enzymes in polymersome nanoreactors for cascade reactions. Angew Chem Int Ed 46:7378–7382
Jia F, Narasimhan B, Mallapragada SK (2013) Biomimetic multienzyme complexes based on nanoscale platforms. AIChE J 59:355–360
Aranaz I, Ramos V, De La Escalera S, Heras A (2003) Co-immobilization of d-hydantoinase and d-carboamylase on Chitin: Application to the Synthesis of p-hydroxyphenylglycine. Biocatal Biotransform 21:349–356
Lopez-Gallego F, Batencor L, Hidalgo A, Mateo C, Fernandez-Lafuente R, Guisan JM (2005) One-pot conversion of cephalosporin c to 7-aminocephalosporanic acid in the absence of hydrogen peroxide. Adv Synth Catal 347:1804–1810
Watanabe J, Ishihara K (2005) Sequential enzymatic reactions and stability of biomolecules immobilized onto phospholipid polymer nanoparticles. Biomacromolecules 7:171–175
Keighron JD, Keating CD (2010) Enzyme: nanoparticle bioconjugates with two sequential enzymes: stoichiometry and activity of malate dehydrogenase and citrate synthase on au nanoparticles. Langmuir 26:18992–19000
Le M, Means GE (1998) NAD +/NADH recycling by coimmobilized lactate dehydrogenase and glutamate dehydrogenase. Enzyme Microbial Technol 23:49–57
Liu W, Zhang S, Wang P (2009) Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration. J Biotechnol 139:102–107
Shi J, Zhang L, Jiang Z (2011) Facile construction of multicompartment multienzyme system through layer-by-layer self-assembly and biomimetic mineralization. ACS Appl Mater Interfaces 3:881–889
Cao X, Li Y, Zhang Z, Yu J, Qian J, Liu S (2012) Catalytic activity and stability of glucose oxidase/horseradish peroxidase co-confined in macroporous silica foam. Analyst 137:5785–5791
Qiu H, Li Y, Ji G, Zhou G, Huang X, Qu Y, Gao P (2009) Immobilization of lignin peroxidase on nanoporous gold: Enzymatic properties and in situ release of H2O2 by co-immobilized glucose oxidase. Bioresour Technol 100:3837–3842
El-Zahab B, Jia H, Wang P (2004) Enabling multienzyme biocatalysis using nanoporous materials. Biotechnol Bioeng 87:178–183
Wang P, Ma G, Gao F, Liao L (2005) Enabling multienzyme bioactive systems using a multiscale approach. China Particuology 3:304–309
Zhang Y, Gao F, Zhang S-P, Su Z-G, Ma G-H, Wang P (2011) Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration. Bioresour Technol 102:1837–1843
Obert R, Dave BC (1999) Enzymatic conversion of carbon dioxide to methanol: enhanced methanol production in silica sol−gel matrices. J Am Chem Soc 121:12192–12193
Xu S-w, Lu Y, Li J, Jiang Z-Y, Wu H (2006) Efficient conversion of CO2 to methanol catalyzed by three dehydrogenases co-encapsulated in an alginate − Silica (ALG − SiO2) hybrid gel. Ind Eng Chem Res 45:4567–4573
El-Zahab B, Donnelly D, Wang P (2008) Particle-tethered NADH for production of methanol from CO2 catalyzed by coimmobilized enzymes. Biotechnol Bioeng 99:508–514
Sun Q, Jiang Y, Jiang Z, Zhang L, Sun X, Li J (2009) Green and efficient conversion of CO2 to methanol by biomimetic coimmobilization of three dehydrogenases in protamine-templated Titania. Ind Eng Chem Res 48:4210–4215
Jiang Y, Sun Q, Zhang L, Jiang Z (2009) Capsules-in-bead scaffold: a rational architecture for spatially separated multienzyme cascade system. J Mater Chem 19:9068–9074
Dibenedetto A, Stufano P, Macyk W, Baran T, Fragale C, Costa M, Aresta M (2012) Hybrid technologies for an enhanced carbon recycling based on the enzymatic reduction of CO2 to methanol in water: chemical and photochemical NADH regeneration. ChemSusChem 5:373–378
Shi J, Wang X, Jiang Z, Liang Y, Zhu Y, Zhang C (2012) Constructing spatially separated multienzyme system through bioadhesion-assisted bio-inspired mineralization for efficient carbon dioxide conversion. Bioresour Technol 118:359–366
Wu M, He Q, Shao Q, Zuo Y, Wang F, Ni H (2011) Preparation and characterization of monodispersed microfloccules of TiO2 nanoparticles with immobilized multienzymes. ACS Appl Mater Interfaces 3:3300–3307
Zhang L, Shi J, Jiang Z, Jiang Y, Meng R, Zhu Y, Liang Y, Zheng Y (2011) Facile preparation of robust microcapsules by manipulating metal-coordination interaction between biomineral layer and bioadhesive layer. ACS Appl Mater Interfaces 3:597–605
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Ardao, I., Hwang, E.T., Zeng, AP. (2013). In Vitro Multienzymatic Reaction Systems for Biosynthesis. In: Zeng, AP. (eds) Fundamentals and Application of New Bioproduction Systems. Advances in Biochemical Engineering/Biotechnology, vol 137. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_232
Download citation
DOI: https://doi.org/10.1007/10_2013_232
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-41520-3
Online ISBN: 978-3-642-41521-0
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)