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Prospective Isolation and Characterization of Human Bone Marrow-Derived MSCs

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Mesenchymal Stem Cells - Basics and Clinical Application I

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 129))

Abstract

There is an increasing interest in adult stem cells, especially mesenchymal stem/stromal cells (MSCs), in hematology and regenerative medicine because of the simplicity of isolation and ex vivo expansion of these cells. Conventionally, MSCs are functionally isolated from tissue based on their capacity to adhere to the surface of culture flasks. This isolation procedure is hampered by the unpredictable influence of secreted molecules and interactions with co-cultured hematopoietic and other unrelated cells, as well as by the arbitrarily selected removal time of non-adherent cells prior to the expansion of MSCs. Finally, functionally isolated cells do not provide biological information about the starting population. To circumvent these limitations, several strategies have been developed to facilitate the prospective isolation of MSCs based on the selective expression or absence of surface markers. The isolation and ex vivo expansion of these cells require an adequate quality control of the source and product. Here we summarize the most frequently used markers and introduce new targets for antibody-based isolation and characterization of bone marrow-derived MSCs.

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Abbreviations

MSC:

Mesenchymal stem/stromal cell

SSEA-4:

Stage specific embryonic antigen 4

TNAP:

Tissue nonspecific alkaline phosphatase

CFU-F:

Colony forming units—fibroblast

PDGF Rβ:

Platelet derived growth factor receptor beta

NK:

Natural killer cells

CDCP1:

CUB domain-containing protein 1

SSEA-3:

Stage specific embryonic antigen 3

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Authors and Affiliations

  1. Division of Haematology, Immunology, Oncology, Rheumatology, and Pulmonology, Department of Internal Medicine II, University Clinic of Tübingen, Tübingen, Germany

    A. Harichandan, K. Sivasubramaniyan & H.-J. Bühring

  2. Laboratory for Stem Cell Research, Division of Haematology, Oncology, Immunology, and Rheumatology, Department of Internal Medicine II, University Clinic, Otfried-Müller-Str. 10, 72076, Tübingen, Germany

    H.-J. Bühring

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  1. A. Harichandan
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  3. H.-J. Bühring
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Correspondence to H.-J. Bühring .

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Editors and Affiliations

  1. Abt. für Plastische und Handchirurgie, Medizinische Hochschule Hannover, Carl-Neubergstr. 1, Hannover, 30625, Germany

    Birgit Weyand

  2. Integrated Department of Oncology, Haematology and Respiratory Diseases, University of Modena, Modena, Italy

    Massimo Dominici

  3. Klinik für Frauenheilkunde und Geburtshi, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, Hannover, 30625, Germany

    Ralf Hass

  4. Klinik für Immunologie und Rheumatologie, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, Hannover, 30625, Niedersachsen, Germany

    Roland Jacobs

  5. Department für Biotechnologie, Universität für Bodenkultur, Muthgasse 18, Wien, 1190, Austria

    Cornelia Kasper

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© 2012 Springer-Verlag Berlin Heidelberg

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Harichandan, A., Sivasubramaniyan, K., Bühring, HJ. (2012). Prospective Isolation and Characterization of Human Bone Marrow-Derived MSCs. In: Weyand, B., Dominici, M., Hass, R., Jacobs, R., Kasper, C. (eds) Mesenchymal Stem Cells - Basics and Clinical Application I. Advances in Biochemical Engineering/Biotechnology, vol 129. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2012_147

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