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Fedbatch Culture and Dynamic Nutrient Feeding

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Cell Culture Engineering

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 101))

Abstract

In the past decade, we have seen a rapid expansion in mammalian cell based therapeutic proteinsreaching clinical applications. This increased demand has been met with much increased productivity throughintensive process development. During this time, fedbatch culture processes have emerged as the predominantmode for producing recombinant proteins. In this review, we discuss the fundamentals of fedbatch cultureprocess design, focusing on the use of stoichiometric nutrient requirements for feed medium formulation,and articulating the need and potential means for devising rational dynamic feeding schemes. Incorporationof on-line nutrient measurement will play a key role in further refinement of process control for thedevelopment of a much sought after generic feeding strategy that can respond to the changing demandsof different cell lines in a fluctuating culture environment. The future of process engineering willlikely require a combination of current process engineering strategies along with a better understandingand control over cell physiology. Process development will likely to entail not only optimizing traditionalengineering parameters but also engineering cell lines with desired characteristics. The integration ofcell engineering and process intensification will likely provide the stimuli that propel the limits of growthand productivity to the next high level.

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Acknowledgments

The support of the cell culture research from Pfizer, Inc., Bayer Health Care, and Merck Co. is gratefully acknowledged. KFW was supported by the NIH Biotechnology Training Grant (GM08347).

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Authors and Affiliations

  1. Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Ave. S.E., 55455-0132, Minneapolis, MN, USA

    Katie F. Wlaschin & Wei-Shou Hu

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  1. Katie F. Wlaschin
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  2. Wei-Shou Hu
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Correspondence to Wei-Shou Hu .

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Wei-Shou Hu

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© 2006 Springer-Verlag Berlin Heidelberg

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Wlaschin, K.F., Hu, WS. (2006). Fedbatch Culture and Dynamic Nutrient Feeding. In: Hu, WS. (eds) Cell Culture Engineering. Advances in Biochemical Engineering/Biotechnology, vol 101. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_015

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