Abstract
We have studied the effect of nitrogen supply on growth as well as nitrate assimilation in Lotus japonicus plants. High nitrate concentrations (higher than 20 mM) were toxic for L japonicus, inhibiting growth, with the optimum nitrate concentration ranging between 4 and 8 mM. The maximum growth and levels of nitrate reductase (NR) and nitrite reductase (NiR) activity as well as nitrate accumulation was obtained in plants grown simultaneously on nitrate plus ammonium. L japonicus could also grow on ammonium as the sole nitrogen source, without a supplement of organic acids like succinate. Low external nitrate concentrations induced a higher growth of roots, while higher external nitrate concentration inhibited the growth of roots and favoured shoots growth. NR and NiR activities were always higher in roots than in shoots of L japonicus plants. An increase in external nitrate concentrations did not alter significantly the plant partitioning of nitrate assimilation among roots and shoots, being predominantly higher in the roots than in stems and leaves. The kinetics of NR activity induction by nitrate showed a different pattern in roots and in leaves, being this induction in roots independent on external nitrate concentration in the short-term. In contrast, the induction kinetics of NiR activity and nitrate accumulation showed the same pattern in roots and leaves, being dependent on the external nitrate concentration. In all cases the levels of NR and NiR activity and nitrate accumulation were higher in roots than in leaves. Significant levels of NR protein but no NR activity were detected in NH +4 -grown plants.
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References
Andrews M. (1986b) The partitioning of nitrate assimilation between root and shoot of higher plants. Plant, Cell and Environment 9, 511–586.
Agrell D, Oscarson, P. and Larsson C-M (1994) Translocation of N to and from barley roots: its dependence on local nitrate supply in split-root cultures. Physiologia Plantarum 90, 467–474.
Agren GI and Ingestad T. (1987) Root: shoot is a balance between nitrogen productivity and photosynthesis. Plant, Cell and Environment, 10, 579–586.
Back E, Burkhardt W, Moyer M, Privalle L and Rothstein S. (1988) Isolation of cDNA clones coding for nitrite reductase: complete sequence and nitrate induction. Molecular General Genetics 212, 20–26.
Beavers L, and Hageman RH. (1980) Nitrate and nitrite reduction. In: The Biochemistry of Plants (B.J. Miflin, Ed). New York: Academic Press, vol. 5, pp. 115–168.
Bloom AJ, Chapin FS, and Mooney HA. (1985) Resource limitation in plants-an economic analogy. Annual Review of Ecology and. Systematics 16, 363–392.
Bradford MM. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.
Brower R. (1962) Nutrient influences on the distribution of the dry matter in the plant. Nhetherlands Joural of Agricultural Science 10, 399–408.
Brower R. (1963) Some aspect of the equilibrium between overground and underground plant parts. Jaarbook Instituut Biologisch Scheikundig Onderzoeg, pp. 31–39.
Caba JM, Lluch C, and Ligero F. (1995) Distribution of nitrate reductase activity in Vicia faba: Effect of nitrate supplies. Physiologia Plantarum 93, 667–672.
Campbell WH. (2002) Molecular control of nitrate reductase and other enzymes involved in nitrate assimilation. In: Photosynthetic nitrogen assimilation and associated carbon and respiratory metabolism (Foyer CH, and Noctor G, Eds.), Kluwer Academic Publishers, Dordrecht, pp.35–48.
Cataldo DA, Haroon M, Schrader LE, and Youngs VL (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in. Soil Science and Plant Analysis 6, 71–80.
Chu CC, Coleman JS, and Mooney HA. (1992) Control of biomass partitioning between roots and shoots: atmospheric carbon dioxide enrichment and the acquisition and allocation of carbon and nitrogen in wild radish. Oecologia 89, 580–587.
Clarkson DT. (1986) Regulation of the absorption and release of nitrate by plant cells: A review of current ideas and methodology. In: Fundamental, Ecological and Agricultural Aspects of Nitrogen Metabolismin Higher Plants (Lambers H, Neeteson JJ, Stulen I, Eds.). Dordrecht: Martinus Nijhoff Publisher, pp. 3-27.
Colebatch G, Trebaskis B, and Udvardi M. (2002) Symbiotic nitrogen fixation in the postgenomics era. New Phytologist 153, 37–42.
Cooper HD, and Clarkson DT. (1989) Cycling of amino-nitrogen and other nutrients between shoots and roots in cereals: a possible mechanism integrating shoot and root in the regulation of nutrient uptake. Journal of Experimental Botany 40, 753–762.
Cox WJ and Reisenauer HM. (1973) Growth and ion uptake by wheat supplied nitrogen as nitrate, or ammonium, or both. Plant and Soil 38, 363–380.
Crawford NM. (1995) Nitrate: nutrient and signal for plant growth. Plant Cell 7, 859–868.
Daniel-Vedele F and Caboche M. (1996) Molecular analysis of nitrate assimilation in higher plants. Comptes Rendus de l’Académie des Sciences de Paris, Sciences de la vi 319, 961–968.
Debeys D, Cammaerts D, and Jacobs M. (1990) Isolation of mutants resistant to high nitrate concentrations. In: Abstracts International Symposium on Nitrate Assimilation, Molecular and Genetics Aspects, Bombannes, INRA-Versalles, France, pp. 118.
De la Rosa MA, Márquez AJ, and Vega JM. (1982) Dissociation of FAD from NADH-nitrate reductase complex from Ankistrodesmus braunii and role of flavin in catalysis. Zeitschrift fur Naturforschung C 37, 24–30.
Ericsson T. (1995) Growth and shoot:root allocation of seedling in relation to nutrient availability. Plant and Soi, 168, 205–214.
Farrar JF, and Williams JHH. (1991) Control of rate of respiration in roots: compartmentation, demand and supply of substrate. In: Compartmentation of Plant Metabolism in Non-photosynthetic Tissues (Emes MJ, Ed.). New York: Cambridge University Press, pp. 167–188.
Fichtner K, and Schulze E-D. (1992) The effect of nitrogen nutrition on annuals originating from habitats of different nitrogen availability. Oecologia, 92, 236–241.
Forde BG. (2002) Local and long-range signalling pathways regulating plant responses to nitrate. Annual Review of Plant Biology 53, 203–224.
Forde BG. and Clarkson DT. (1999) Nitrate and ammonium nutrition of plants: physiological and molecular perspectives. In: Advances in Botanical Research, Vol. 30, Academic Press, New York, pp. 1–90.
Gojon A, Bussi C, Grignon C, and Salsac L. (1991) Distribution of NO − 3 reduction between roots and shoots of peach tree seedlings as affected by NO − 3 uptake rate. Physiologia Plantarum 82, 502–512.
Gojon A, Plassard C, and Bussi C. (1994) Root/shoot distribution of NO − 3 assimilation in herbaceous and woody species. In: A Whole Plant Perpective on Carbon-Nitrogen Interaction (Roy J, and Garnier E, Eds.). The Hague: SPB Academic Publishing, pp. 131–147.
Heldt HW. (1997) Nitrate assimilation. In: Plant Biochemestry and molecular Biology (Heldt, HW, Ed.). New York: Oxford University Press, pp. 247–276.
Hirel B., and Lea PJ. (2002) The Biochemistry, Molecular Biology, and Genetic manipulation of primary ammonia assimilation. In: Photosynthetic nitrogen assimilation and associated carbon and respiratory metabolism. (Foyer CH, and Noctor G, Eds.), Kluwer Academic Publishers, Dordrecht, pp. 71–92.
Hoff T, Troung H-N., and Caboche M. (1994) The use of mutants and transgenic plants to study nitrate assimilation. Plant Cell and Evironment 17, 489–506.
Huber, SC. (1983) Relation between photosynthetic starch formation and dry-weight partitioning between the shoot and root. Canadian Journal of Botany 61, 2709–2716.
Ireland RJ., and Lea PJ. (1999) The enzymes of glutamine, glutamate, asparagine, and aspartate metabolism. In: Plant Amino Acids: Biochemistry and Biotechnology. (Singh, BK, Ed.). New York: Marcel Dekker, Inc, pp. 49–109.
Jackson WA, Volk RJ, and Israel DW. (1980) Energy supply and nitrate assimilation in root systems. In: Carbon-Nitrogen Interaction in Crop Production (Tanaka, Ed.). Tokyo: Japan Society for Promotion of Science, pp. 25–40.
Kaiser WM, and Brendle-Behnisch E. (1991) Rapid modulation of spinach leaf nitrate reductase activity by photosynthesis. I. Modulation in vivo by CO 2 availability. Plant Physiology 96, 363–367.
Komor E. (1994) Regulation of futile cycles: the transport of carbon and nitrogen in plants. In: Flux Control in Biological System (Schulze, E-D, Ed). San Diego, CA: Academic Press, pp. 153–202.
Kramer V, Lahners K, Back E, Privalle LS, and Rothstein S. (1989) Transient accumulation of nitrite reductase mRNA in maiz following the addition of nitrate. Plant Physiology 90 1214–1220.
Laemmli UK. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 251, 614–616.
Lewis OAM, and Chadwick S. (1983) A 15N investigation into nitrogen assimilation in hydroponically growth barley (Hordeum vulgare L cv. Clipper) in response to nitrate, ammonium and mixed nitrate and ammonium nutrition. New Phytologist 95, 653–646.
Neininger A, Kronenberger J, and Mohr H. (1992) Co-action of light, nitrate, and a plastidic factor in controlling nitrite-reductase gene expression in tobacco. Planta 187, 381–387.
Oaks A. (1992) A re-evaluation of nitrogen assimilation in roots. Bioscience 42, 103–111.
Oostinder-Braaksma FJ, and Feenstra WJ. (1973) Isolation and characterization of chlorate-resistant mutants of Arabidopsis thaliana. Mutation Research 19, 175–185.
Orea A, Pajuelo P, Pajuelo E, Márquez AJ and Romero JM (2001) Characterisation and expression studies of a root cDNA encoding for ferredoxin-nitrite reductase fromM Lotus japonicus. Physiologia Plantarum 113, 193–202.
Pajuelo E, Borrero, JA, and Márquez AJ (1993) Immunological approach to subunit composition of ferredoxin-nitrite reductase from Chamydomonas reinhardtii. Plant Science 95, 9–21.
Pajuelo E, Pajuelo P, Clemente MT and Márquez AJ (1995) Regulation of expression of ferredoxin-nitrite reductase in synchronous culture of Chlamydomonas reinhardtii. Biochimica et Biophysica Acta, 1249, 72–78.
Pajuelo P, Pajuelo E, Orea A, Romero JM, and Márquez AJ (2002) Influence of plant age and growth conditions on nitrate assimilation in roots of Lotus japonicus plants. Functional. Plant Biology 29, 485–494.
Pelsy F. and Caboche M. (1992) Molecular genetics of nitrate reductase in higher plants. Advances in Genetics 30, 1–40.
Pelsy F, Kronenberger J, and Caboche M. (1991) M2 seed screening for nitrate reductase deficiency in Nicotiana plumbaginifolia. Plant Science 76, 109–114.
Peuke AD, Glaab J, Kaiser, WM and Jeschlke WD. (1996) The uptake and flow of C and n and ions between roots and shoots in Ricinus communis L IV. Flow and metabolism of inorganic nitrogen and malate depending on nitrogen nutrient and salt treatment. Journal of Experimental Botany 47, 377–385.
Pilbeam DJ, and Kirby EA. (1992) Some aspects of the utilization of nitrate and ammonium by plants. In: Nitrogen Metabolism of Plants (Mengel K, and Pilbeam, DJ, Eds.). Oxford: Oxford Science Publications, pp. 55–70.
Radutoiu S, Madsen LH, Madsen EB, Felle HH, Umehara Y, Gronlund M, Sato S, Nakamuro Y, Tabata S, Sandal N, and Stougaard J. (2003) Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases. Nature 425, 585–591.
Redinbaugh MG, and Campbell WM. (1991) Higher plant responses to environmental nitrate. Physiologia Plantarum 82, 640–650.
Rufty T.W. (1997) Probing the carbon and nitrogen interaction: a whole plant perspective. In: A Molecular Approach to Prymary Metabolism in Higher Plants (Foyer CH and Quick WP, Eds.). London: Taylor & Francis, pp. 255–273.
Rufty TW, Huber, SC, and Volk, RJ (1988) Alterations in leaf carbohydrate metabolism in response of nitrogen stress. Plant Physiology 88, 725–730.
Scheible W-R, Lauerer M, Schulze E-D, Caboche M, and Stitt M. (1997) Accumulation of nitrate in the shoot acts as a signal to regulate shoo-root allocation in tobacco. The Plant Journal 11, 671–691.
Schulze E-D, and Chapin FS. (1987) Plant specialization to environments of different resource availability. In: Potential and Limitations of Ecosystem Analysis, Ecological Studies (E.-D. Schulze and H. Zwölfer, Eds.). Berlin: Springer-Verlag, vol. 61, pp. 120–148.
Shaner DL, and Boyer J.S. (1976) Nitrate reductase in maiz (Zea mays L) leaves. I. Regulation by nitrate flux. Plant Physiology 58, 499–504.
Smirnoff N, and Stewart GR. (1985) Nitration assimilation and translocation by higher plants: Comparative physiology and ecological consequences. Physiologia Plantarum 64, 133–140.
Stitt M. (1999) Nitrate regulation of metabolism and growth. Current Opinion in. Plant Biology 2, 178–86.
Stougaard J, Szczyglowski, K, de Brujin FJ, and Parniske M. (1999) Genetic nomenclature guidelines for the model legume Lotus japonicus. Trends in Plant Science 4, 300–301.
Szczyglowski, K, Hamburger D, Kapronov P, and Brujin FJ. (1997) Construction of a Lotus japonicus late nodulin expressed sequence tag library and identification of novel nodule specific genes. Plant Physiology 114, 1335–1346.
Wagner BM, and Beck E. (1993) Cytokinins in the perennial herb Urtica dioca L as influenced by its nitrogen status. Planta 190, 511–518.
Wallace W. (1986) Distribution of nitrate assimilation between the root and shoot of legumes and a comparison with wheat. Physiologia Plantarum 66, 630–636.
Woodall J. (1994) Studies of glutamine synthetase in Lotus japonicus species and characterization of the occurrence of root GS2 in other legumes. PhD thesis, University College, London.
Woodall J and Forde BG. (1996) Glutamine synthetase polypeptide in the roots of 55 legume species in relation to their climatic origin and partitioning of nitrate assimilation. Plant Cell and Environ, 19, 848–858.
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Orea, A., Pajuelo, P., Romero, J.M., Márquez, A.J. (2005). Nitrate assimilation: Influence of nitrogen supply. In: Márquez, A.J. (eds) Lotus japonicus Handbook. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3735-X_30
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