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Cell and Tissue Microdissection in Combination with Genomic and Proteomic Applications

  • Chapter
Neuroanatomical Tract-Tracing 3

Abstract

The combination of tissue microdissection protocols including discrete cell microaspiration and laser capture microdissection with high throughput gene expression profiling platforms such as cDNA microarrays and oligonucleotide microarrays enables the simultaneous assessment of many individual elements from a single cell or a population of homogeneous cells. This chapter outlines in detail the theoretical and practical background for selecting the appropriate tissues and conditions amenable to expression profiling. In addition, this report illustrates the usage of microdissection strategies and RNA amplification methodologies in concert with array technologies using tissues harvested from the central nervous system obtained from animal models of neurodegeneration and postmortem human brain tissues.

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Authors and Affiliations

  1. Center for Dementia Research, Nathan Kline Institute, and Departments of Psychiatry and Physiology and Neuroscience, New York University School of Medicine, Orangeburg, NY, 10962

    Stephen D. Ginsberg

  2. Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, NC, 27157

    Scott E. Hemby

  3. Department of Neurological Sciences, Rush University Medical Center, Chicago, IL, 60612

    Elliott J. Mufson

  4. Division of Neuropathology, Departments of Pathology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, 21205

    Lee J. Martin

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  1. Stephen D. Ginsberg
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  2. Scott E. Hemby
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  3. Elliott J. Mufson
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  4. Lee J. Martin
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Editors and Affiliations

  1. Center for Molecular and Behavioral Neuroscience, Rutgers University, Newark, NJ, USA

    Laszlo Zaborszky

  2. Department of Anatomy, Vrije University Medical Center, Amsterdam, The Netherlands

    Floris G. Wouterlood

  3. Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain

    José Luis Lanciego

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Ginsberg, S.D., Hemby, S.E., Mufson, E.J., Martin, L.J. (2006). Cell and Tissue Microdissection in Combination with Genomic and Proteomic Applications. In: Zaborszky, L., Wouterlood, F.G., Lanciego, J.L. (eds) Neuroanatomical Tract-Tracing 3. Springer, Boston, MA . https://doi.org/10.1007/0-387-28942-9_4

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