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Bacteriophage SPP1 DNA Packaging

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Viral Genome Packaging Machines: Genetics, Structure, and Mechanism

Part of the book series: Molecular Biology Intelligence Unit ((MBIU))

Concluding Remarks

The phenomenology of bacteriophage SPP1 DNA packaging is well known and some of the molecular mechanisms involved are among the best understood in tailed phages systems. These include the terminase recognition and cleavage of its target sequence pac, the structure and function of the portal protein, the mechanism of headful sensor, and connector assembly. Other aspects of the DNA packaging process were not yet studied in detail like the terminase-procapsid interaction, the properties and assembly of the DNA translocating complex, or the mechanism of DNA translocation. A complete picture of the DNA packaging process requires knowledge of all these molecular mechanisms and their integration in the dynamics of the infected cell. An interdisciplinary approach combining genetics, biochemistry, structural biology and bacterial cell biology, on one side, and comparative analysis of the strategies used in different virus systems, on the other side, will undoubtedly be necessary to characterise the unifying mechanism optimised by tailed phages and herpesviruses to encapsidate their viral genome.

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Author information

Authors and Affiliations

  1. Department of Neuroproteomics, Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin-Buch, Germany

    Anja Dröge

  2. Unité de Virologie Moléculaire et Structurale, Gif-sur-Yvette, France

    Paulo Tavares

Authors
  1. Anja Dröge
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  2. Paulo Tavares
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Dröge, A., Tavares, P. (2005). Bacteriophage SPP1 DNA Packaging. In: Viral Genome Packaging Machines: Genetics, Structure, and Mechanism. Molecular Biology Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-28521-0_6

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