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Lanthanide-Labeled DNA

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Topics in Fluorescence Spectroscopy

Part of the book series: Topics in Fluorescence Spectroscopy ((TIFS,volume 7))

Conclusion

Lanthanides, as alternative probes to conventional fluorophores, can lead to enhanced sensitivity in applications where autofluorescence is a problem. Multiple labeling with lanthanide chelates have been shown to be an effective method for further increases in sensitivity. These probes, being nonisotopic, avoid the many practical problems associated with radioactive probes. Lanthanide probes are particularly advantageous in resonance energy transfer, whether for measuring distances in biocomplexes or when used to generate new lifetime tailored dyes.

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  1. Physics Department and Biophysics Center, University of Illinois, Urbana, Illinois, 61801

    Paul R. Selvin

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  1. Center for Fluorescence Spectroscopy and Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland

    Joseph R. Lakowicz

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Selvin, P.R. (2003). Lanthanide-Labeled DNA. In: Lakowicz, J.R. (eds) Topics in Fluorescence Spectroscopy. Topics in Fluorescence Spectroscopy, vol 7. Springer, Boston, MA. https://doi.org/10.1007/0-306-47947-8_6

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