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Abstract

Cell division is the final step of cell reproduction as a well-grown cell divides into daughter cells. However, this phenomenon cannot occur by itself and requires preceding cellular events. A cell must essentially duplicate its mass, namely the amount of small and large biomolecules, if the cell is going to divide by binary fission. The replication of chromosomal DNA is done with the highest priority; however, it should occur in synchronicity with other reproduction events. The replicated chromosomes must also be partitioned for delivery to the daughter cells properly. Cytokinesis, the final step of cell division, may depend on the formation of a special apparatus. Mycoplasmas have the distinct ability to adhere to cell surfaces of their hosts. For some mycoplasma species, this ability depends on a terminal structure located at a cell pole. Duplication of this structure before cell division is for them an indispensable event. In this chapter, individual steps of cell division will be discussed focusing on recent studies.

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References

  1. Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts, and J. D. Watson. 1994. Molecular biology of the cell., Third ed. Garland Publishing, Inc., New York & London.

    Google Scholar 

  2. Baseman, J. B., R. M. Cole, D. C. Krause, and D. K. Leith. 1982. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J. Bacteriol. 151:1514–1522.

    PubMed Central  PubMed  CAS  Google Scholar 

  3. Bi, E. F., and J. Lutkenhaus. 1991. FtsZ ring structure associated with division in Escherichia coli. Nature. 354:161–4.

    Article  PubMed  CAS  Google Scholar 

  4. Bramhill, D., and A. Kornberg. 1988. A model for initiation at origins of DNA replication. Cell. 54:915–8.

    Article  PubMed  CAS  Google Scholar 

  5. Bredt, W. 1968. Motility and multiplication of Mycoplasma pneumoniae. A phase contrast study. Pathol. Microbiol. 32:321–6.

    CAS  Google Scholar 

  6. Chambaud, I., R. Heilig, S. Ferris, V. Barbe, D. Samson, F. Galisson, I. Moszer, K. Dybvig, H. Wroblewski, A. Viari, E. P. Rocha, and A. Blanchard. 2001. The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis. Nucleic Acids Res. 29:2145–53.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  7. Errington, J. 1998. Dramatic new view of bacterial chromosome segregation. ASM News. 64:210–217.

    Google Scholar 

  8. Feldner, J., U. Göbel, and W. Bredt. 1982. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature. 298:765–7.

    Article  PubMed  CAS  Google Scholar 

  9. Fisseha, M., H. W. Gohlmann, R. Herrmann, and D. C. Krause. 1999. Identification and complementation of frameshift mutations associated with loss of cytadherence in Mycoplasma pneumoniae. J. Bacteriol. 181:4404–10.

    PubMed Central  PubMed  CAS  Google Scholar 

  10. Fraser, C. M., J. D. Gocayne, O. White, M. D. Adams, R. A. Clayton, R. D. Fleischmann, C. J. Bult, A. R. Kerlavage, G. Sutton, J. M. Kelley, R. D. Fritchman, J. F. Weidman, K. V. Small, M. Sandusky, J. Fuhrmann, D. Nguyen, R. Utterback, D. M. Saudek, C. A. Phillips, J. M. Merrick, J.-F. Tomb, B. A. Dougherty, K. F. Bott, P.-C. Hu, T. S. Lucier, S. N. Peterson, H. O. Smith, C. A. Hutchison III, and J. C. Venter. 1995. The minimal gene complement of Mycoplasma genitalium. Science. 270:397–403.

    Article  PubMed  CAS  Google Scholar 

  11. Fujita, M. Q., H. Yoshikawa, and N. Ogasawara. 1992. Structure of the dnaA and DnaA-box region in the Mycoplasma capricolum chromosome: conservation and variations in the course of evolution. Gene. 110:17–23.

    Article  PubMed  CAS  Google Scholar 

  12. Garnier, M., M. Clerc, and J. M. Bove. 1984. Growth and division of Spiroplasma citri: elongation of elementary helices. J. Bacteriol. 158:23–8.

    PubMed Central  PubMed  CAS  Google Scholar 

  13. Garnier, M., M. Clerc, and J. M. Bove. 1981. Growth and division of spiroplasmas: morphology of Spiroplasma citri during growth in liquid medium. J. Bacteriol. 147:642–52.

    PubMed Central  PubMed  CAS  Google Scholar 

  14. Glass, J. I., E. J. Lefkowitz, J. S. Glass, C. R. Heiner, E. Y. Chen, and G. H. Cassell. 2000. The complete sequence of the mucosal pathogen Ureaplasma urealyticum. Nature. 407:757–62.

    Article  PubMed  CAS  Google Scholar 

  15. Göbel, U., V. Speth, and W. Bredt. 1981. Filamentous structures in adherent Mycoplasma pneumoniae cells treated with nonionic detergents. J. Cell Biol. 91:537–543.

    Article  PubMed  Google Scholar 

  16. Helmstetter, F. C. 1996. Timing of synthetic activities in the cell cycle, p. 1627–1639. In F. C. Neidhardt (ed.), Escherichia coli and Salmonella. ASM press, Washington D. C.

    Google Scholar 

  17. Hilbert, H., R. Himmelreich, H. Plagens, and R. Herrmann. 1996. Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes. Nucleic Acids Res. 24(4):628–39.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  18. Himmelreich, R., H. Hilbert, H. Plagens, E. Pirkl, B.-C. Li, and R. Herrmann. 1996. Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae. Nucleic Acids Res. 24:4420–4449.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  19. Hiraga, S. 2000. Dynamic localization of bacterial and plasmid chromosomes. Annu. Rev. Genet. 34:21–59.

    Article  PubMed  CAS  Google Scholar 

  20. Hu, P. C, R. M. Cole, Y. S. Huang, J. A. Graham, D. E. Gardner, A. M. Collier, and W. A. Clyde, Jr. 1982. Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 216:313–5.

    Article  PubMed  CAS  Google Scholar 

  21. Kirchhoff, H. 1992. Motility, p. 289–306. In J. Maniloff, R. N. McElhaney, L. R. Finch, and J. B. Baseman (ed.), Mycoplasmas- Molecular Biology and Pathogenesis. American Society for Microbiology, Washington, D.C.

    Google Scholar 

  22. Krause, D. C. 1998. Mycoplasma pneumoniae cytadherence: organization and assembly of the attachment organelle. Trends in Microbiol. 6:15–18.

    Article  CAS  Google Scholar 

  23. Krause, D. C. 1996. Mycoplasma pneumoniae cytadherence: unravelling the tie that binds. Mol. Microbiol. 20:247–53.

    Article  PubMed  CAS  Google Scholar 

  24. Krause, D. C, and M. F. Balish. 2001. Structure, function, and assembly of the terminal organelle of Mycoplasma pneumoniae. FEMS Microbiol. Lett. 198:1–7.

    Article  PubMed  CAS  Google Scholar 

  25. Krause, D. C, D. K. Leith, R. M. Wilson, and J. B. Baseman. 1982. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect. Immun. 35:809–17.

    PubMed Central  PubMed  CAS  Google Scholar 

  26. Kukekova, A. V., A. Y. Malinin, J. A. Ayala, and S. N. Borchsenius. 1999. Characterization of Acholeplasma laidlawii ftsZ gene and its gene product. Biochem Biophys Res. Commun. 262:44–9.

    CAS  Google Scholar 

  27. Layh-Schmitt, G., and M. Harkenthal. 1999. The 40- and 90-kDa membrane proteins (ORF6 gene product) of Mycoplasma pneumoniae are responsible for the tip structure formation and PI (adhesin) association with the Triton shell. FEMS Microbiol Lett. 174:143–9.

    Article  PubMed  CAS  Google Scholar 

  28. Layh-Schmitt, G., and R. Herrmann. 1994. Spatial arrangement of gene products of the PI operon in the membrane of Mycoplasma pneumoniae. Infect. Immun. 62:974–9.

    PubMed Central  PubMed  CAS  Google Scholar 

  29. Layh-Schmitt, G., R. Himmelreich, and U. Leibfried. 1997. The adhesin related 30-kDa protein of Mycoplasma pneumoniae exhibits size and antigen variability. FEMS Microbiol. Lett. 152:101–8.

    Article  PubMed  CAS  Google Scholar 

  30. Layh-Schmitt, G., A. Podtelejnikov, and M. Mann. 2000. Proteins complexed to the PI adhesin of Mycoplasma pneumoniae. Microbiology. 146:741–747.

    PubMed  CAS  Google Scholar 

  31. Lowe, J., and L. A. Amos. 1998. Crystal structure of the bacterial cell-division protein FtsZ. Nature. 391:203–6.

    Article  PubMed  CAS  Google Scholar 

  32. Lutkenhaus, J., and S. G. Addinall. 1997. Bacterial cell division and the Z ring. Annu. Rev. Biochem. 66:93–116.

    Article  PubMed  CAS  Google Scholar 

  33. Maddock, J. R., and L. Shapiro. 1993. Polar location of the chemoreceptor complex in the Escherichia coli cell. Science. 259:1717–23.

    Article  PubMed  CAS  Google Scholar 

  34. Margolin, W. 1999. The bacterial cell divisin machine. ASM News. 65:137–143.

    Google Scholar 

  35. Meng, K. E., and R. M. Pfister. 1980. Intracellular structures of Mycoplasma pneumoniae revealed after membrane removal. J. Bacteriol. 144:390–399.

    PubMed Central  PubMed  CAS  Google Scholar 

  36. Miyata, M., and T. Fukumura. 1997. Asymmetrical progression of replication forks just after initiation on Mycoplasma capricolum chromosome revealed by two-dimensional gel electrophoresis. Gene. 193:39–47.

    Article  PubMed  CAS  Google Scholar 

  37. Miyata, M., W. S. Ryu and H.C. Berg. 2002. Force and velocity of Mycoplasma mobile gliding. J. Bacteriol. 184: in press

    Google Scholar 

  38. Miyata, M., K. Sano, R. Okada, and T. Fukumura. 1993. Mapping of replication initiation site in Mycoplasma capricolum genome by two-dimensional gel-el ectrophoretic analysis. Nucleic Acids Res. 21:4816–23.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  39. Miyata, M., and S. Seto. 1999. Cell reproduction cycle of mycoplasma. Biochimie. 81:873–8.

    Article  PubMed  CAS  Google Scholar 

  40. Miyata, M., L. Wang, and T. Fukumura. 1993. Localizing the replication origin region on the physical map of the Mycoplasma capricolum genome. J. Bacteriol. 175:655–660.

    PubMed Central  PubMed  CAS  Google Scholar 

  41. Miyata, M., L. Wang, and T. Fukumura. 1991. Physical mapping of the Mycoplasma capricolum genome. FEMS Microbiol. Lett. 63:329–334.

    Article  PubMed  CAS  Google Scholar 

  42. Miyata, M., H. Yamamoto, T. Shimizu, A. Uenoyama, C. Citti, and R. Rosengarten. 2000. Gliding mutants of Mycoplasma mobile: relationships between motility and cell morphology, cell adhesion and microcolony formation. Microbiology. 146:1311–1320.

    PubMed  CAS  Google Scholar 

  43. Morowitz, H. J., and J. Maniloff. 1966. Analysis of the life cycle of Mycoplasma gallisepticum. J. Bacteriol. 91:1638–44.

    PubMed Central  PubMed  CAS  Google Scholar 

  44. Popham, P. L., T. W. Hahn, K. A. Krebes, and D. C. Krause. 1997. Loss of HMW1 and HMW3 in noncytadhering mutants of Mycoplasma pneumoniae occurs post-translationally. Proc. Natl. Acad. Sci. USA. 94:13979–84.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  45. Quinlan, D. C, and J. Maniloff. 1972. Membrane association of the deoxyribonucleic acid growing-point region in Mycoplasma gallisepticum. J. Bacteriol. 112:1375–9.

    PubMed Central  PubMed  CAS  Google Scholar 

  46. Razin, S., and E. Jacobs. 1992. Mycoplasma adhesion. J. Gen. Microbiol. 138:407–22.

    Article  PubMed  CAS  Google Scholar 

  47. Regula, J. T., G. Boguth, A. Görg, J. Hegermann, F. Mayer, R. Frank, and R. Herrmann. 2001. Defining the mycoplasma ‘cytoskeleton’: the protein composition of the Triton X-100 insoluble fraction of the bacterium Mycoplasma pneumoniae determined by 2-D gel electrophoresis and mass spectrometry. Microbiology. 147:1045–1057.

    PubMed  CAS  Google Scholar 

  48. Renaudin, J., A. Marais, E. Verdin, S. Duret, X. Foissac, F. Laigret, and J. M. Bove. 1995. Integrative and free Spiroplasma citri oriC plasmids: expression of the Spiroplasmaphoeniceum spiralin in Spiroplasma citri. J. Bacteriol. 177:2870–7.

    PubMed Central  PubMed  CAS  Google Scholar 

  49. Romero-Arroyo, C. E., J. Jordan, S. J. Peacock, M. J. Willby, M. A. Farmer, and D. C. Krause. 1999. Mycoplasma pneumoniae protein P30 is required for cytadherence and associated with proper cell development. J. Bacteriol. 181:1079–87.

    PubMed Central  PubMed  CAS  Google Scholar 

  50. Rosengarten, R., and H. Kirchhoff. 1989. Growth morphology of Mycoplasma mobile 163K on solid surfaces: Reproduction, aggregation, and microcolony formation. Curr. Microbiol. 18:15–22.

    Article  Google Scholar 

  51. Seto, S., G. Layh-Schmitt, T. Kenri, and M. Miyata. 2001. Visualization of the attachment organelle and cytadherence proteins of Mycoplasma pneumoniae by immunofluorescence microscopy. J. Bacteriol. 183:1621–30.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  52. Seto, S., and M. Miyata. 1998. Cell reproduction and morphological changes in Mycoplasma capricolum. J. Bacteriol. 180:256–64.

    PubMed Central  PubMed  CAS  Google Scholar 

  53. Seto, S., and M. Miyata. 1999. Partitioning, movement, and positioning of nucleoids in Mycoplasma capricolum. J. Bacteriol. 181:6073–80.

    PubMed Central  PubMed  CAS  Google Scholar 

  54. Seto, S., S. Murata, and M. Miyata. 1997. Characterization of dnaA gene expression in Mycoplasma capricolum. FEMS Microbiol. Lett. 150:239.

    Article  PubMed  CAS  Google Scholar 

  55. Skarstad, K., and E. Boye. 1994. The initiator protein DnaA: evolution, properties and function. Biochim. Biophys. Acta. 1217:111–130.

    Article  PubMed  CAS  Google Scholar 

  56. Suzuki, K., M. Miyata, and T. Fukumura. 1993. Comparison of the conserved region in the dnaA gene from three mollicute species. FEMS Microbiol. Lett. 114:229–233.

    Article  PubMed  CAS  Google Scholar 

  57. Trachtenberg, S. 1998. Mollicutes-wall-less bacteria with internal cytoskeletons. J. Struct. Biol. 124:244–56.

    Article  PubMed  CAS  Google Scholar 

  58. Wang, X., and J. Lutkenhaus. 1996. Characterization of the ftsZ gene from Mycoplasma pulmonis, an organism lacking a cell wall. J. Bacteriol. 178:2314–9.

    PubMed Central  PubMed  CAS  Google Scholar 

  59. Weisburg, W. G., J. G. Tully, D. L. Rose, J. P. Petzel, H. Oyaizu, D. Yang, L. Mandelco, J. Sechrest, T. G. Lawrence, J. Van Etten, J. Maniloff, and C. R. Woese. 1989. A phylogenetic analysis of the Mycoplasmas: Basis for their classification. J. Bacteriol. 171:6455–6467.

    PubMed Central  PubMed  CAS  Google Scholar 

  60. Wheeler, R. T., and L. Shapiro. 1997. Bacterial chromosome segregation: is there a mitotic apparatus? Cell. 88:577–9.

    Article  PubMed  CAS  Google Scholar 

  61. Wilson, M. H., and A. M. Collier. 1976. Ultrastructural study of Mycoplasma pneumoniae in organ culture. J. Bacteriol. 125:332–9.

    PubMed Central  PubMed  CAS  Google Scholar 

  62. Ye, F., J. Renaudin, J. M. Bove, and F. Laigret. 1994. Cloning and sequencing of the replication origin (oriC) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids. Curr. Microbiol. 29:23–9.

    Article  PubMed  CAS  Google Scholar 

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Miyata, M. (2002). Cell Division. In: Razin, S., Herrmann, R. (eds) Molecular Biology and Pathogenicity of Mycoplasmas. Springer, Boston, MA. https://doi.org/10.1007/0-306-47606-1_6

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  • DOI: https://doi.org/10.1007/0-306-47606-1_6

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