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Extrachromosomal Elements and Gene Transfer

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Molecular Biology and Pathogenicity of Mycoplasmas

Abstract

Molecular dissection of plasmid or phage DNA provided many important clues to central biological processes such as DNA replication and gene expression in bacteria. In recent years, plasmid research has taken a new turn, with the advent of gene cloning, and the use of plasmids as gene vectors. Indeed, the availability of gene transfer systems is an absolute prerequisite to genetic studies. In mollicutes, the engineering of endogenous plasmid and viral DNA to cloning vectors has encountered limited success. However, during the past five years, significant progress in the development of new genetic tools has been accomplished, opening the way to genetic studies in mollicutes. Gene vectors based on artificial oriC plasmids (plasmids carrying the replication origin, oriC of Spiroplasma citri) and on transposons have been constructed, and methods such as transposon mutagenesis and gene disruption through homologous recombination have been used to generate mutants. Characterization of these mutants has contributed to the identification of genes associated with biological function such as motility, cytadherence, and pathogenicity. Exhaustive reviews on plasmids, viruses, and molecular genetics of mollicutes have been published previously7,9,22,23,33,71,85,86,91 Therefore this chapter will focus primarily on the recent data with special emphasis on viruses and plasmids used as tools for gene transfer.

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  1. Laboratoire de Biologie Cellulaire et Moléculaire, UMR GDPP, IBVM, INRA and Université Victor Segalen Bordeaux2, 71 avenue Edouard Bourleaux, BP 81, 33883, Villenave d’Ornon Cedex, France

    Joël Renaudin

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  1. The Hebrew University-Hadassah Medical School, Jerusalem, Israel

    Shmuel Razin

  2. University of Heidelberg, Heidelberg, Germany

    Richard Herrmann

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Renaudin, J. (2002). Extrachromosomal Elements and Gene Transfer. In: Razin, S., Herrmann, R. (eds) Molecular Biology and Pathogenicity of Mycoplasmas. Springer, Boston, MA. https://doi.org/10.1007/0-306-47606-1_15

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  • DOI: https://doi.org/10.1007/0-306-47606-1_15

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