Conclusion
Genotypic differences regarding virulence factors between human and animal E. coli strains were observed in this work. They consisted mainly on the frequencies and type of adhesin encoded operons detected, which may reveal uropathogenic E. coli adaptation to different host cell receptors. Low detection of the cnf1 gene in human hemolytic E. coli strains suggests the possibility that, in these strains, other cytotoxins may be involved in the pathogenesis of urinary tract infection.
Simultaneous presence of 4 or more virulence factors was more important in animal (12/40), than in human (1/90) E. coli strains. This may be due to the fact that these animal strains caused primary urinary tract infection (11/40). Simultaneous carriage of virulence determinants by these strains may occur in fundamental gene blocks, previously designated as “pathogenicity-associated-islands“. This hypothesis is under investigation.
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References
Low, D., Braten, B.A., Ling, G.V., Johnson, D.L., and Ruby, A.L., 1988, Isolation and comparison of Escherichia coli strains from canine and human patients with urinary tract infection. Infect. Immun., 56:2601–2609.
Yuri, K., Nakata, K., Katae, H., Yamamoto, S., and Hasegawa, A., 1998, Distribution of uropathogenic virulence factors among Escherichia coli strains isolated from dogs and cats. J. Vet. Sci. 60:287–290.
Le Bouguenec, C., Archambaud, M., and Labigne, A., 1992, Rapid and specific detection of the pap, afa and sfa adhesin-encoded operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J. Clin. Microbiol. 30:1189–1193.
Yamamoto, C., Terai, A., Yuri, K., Korazano, H., Takeda, Y., and Yoshida, O., 1995, Detection of virulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunol. Med. Microbiol., 12:85–90.
Kao, J.-S., Stucker, D.M., Warren, J.W., and Mobley, H.L.T., 1997, Pathogenicity island sequences of pyelonephritogenic Escherichia coli CFT073 are associated with virulent uropathogenic strains. Infect. Immun. 65:2812–2820.
Guyer, D., Kao, J.-S., and Mobley, H.L.T., 1998, Genomic analysis of a pathogenicity island in uropathogenic Escherichia coli CFT073: distribution of homologous sequences among isolates from patients with pyelonephritis, cystitis and catheter-associated bacteriuria and from fecal samples. Infect. Immun. 66:4411–4417.
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Féria, C.P., Correia, J.D., Gonçalves, J., Machado, J. (2002). Detection of Virulence Factors in Uropathogenic Escherichia Coli Isolated from Humans, Dogs and Cats in Portugal. In: Emoődy, L., Pál, T., Hacker, J., Blum-Oehler, G. (eds) Genes and Proteins Underlying Microbial Urinary Tract Virulence. Advances in Experimental Medicine and Biology, vol 485. Springer, Boston, MA. https://doi.org/10.1007/0-306-46840-9_40
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DOI: https://doi.org/10.1007/0-306-46840-9_40
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