Abstract
This investigation was improve the separation for tauret (retinylidene taurine) and to compare its content in the retina under dark and light adaptation. To prevent tauret hydrolysis, retinal samples were quickly frozen and lyophilized. Methanol extracts of dried retina and pigment epithelium from both darkor light-adapted frogs, Rana ridibunda, were injected onto HPLC. Synthetic standard tauret appeared at 4.7 min after the solvent front. At the same time, an endogenous substance was eluted from the mixed retinal and pigment epithelial samples. The UV spectra of this endogenous compound matched with the spectra of synthetic tauret obtained under identical conditions, with λmax=446 nm at peak. We conclude that the HPLC system used permitted full separation of tauret from the methanol extracts of the retina and pigment epithelium. TLC and further HPLC analysis have shown that tauret quantities were several times higher in the retina and pigment epithelium of the frogs adapted to dark compared with those light-adapted (about 4 h under 1000 1x illumination). Tauret based vitamin A transport is probably involved in other systems as well, where along with its other known beneficial effects taurine probably is necessary to facilitate vitamin A transport.
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References
Altshuler, D., Lo Turco, J.J., Rush, J., and Cepko, C. 1993, Taurine promotes the differentiation of a vertebrate retinal cell type in vitro, Development, 119: 1317–1328.
Awapara J. 1962 Free amino acids in invertebrates: a comparative study of their distribution and metabolism In Amino Acid Pools pp. 158–175, Elsevier Publ Company Amsterdam, London, New York
Chesney R.W., Helms R.A., Christensen M., Budreau A. M., Han X. & Sturman J.A., 1998, The role of taurine in infant nutrition. In: “Taurine: Basic and Clinical Aspects” (Ed. by Schaffer S., Lombardinin B., & Huxtable R.) p.463–476. Plenum Publ. Corp.
Dontsov, A. E., Sakina, N. L., Boulton, M. A., 1991, Taurine stimulates phagocytosisin retinal pigment epithelium. Biol. Membranes, (Russ.) 8: 1197–1198
Dumoulin J.C., Evers J.L., Bras M., Pieters M.H., and Geraedts J.P., 1992. Positive effect of taurine on preimplantation development of mouse embryos in vitro. J. Reprod. Fertil. 94:373–380
Eichmuller S., Schaffer S., 1995. Sensory neuron development revealed by taurine immunocytochemistry in the honeybee. J. comp. Neurol. 352:297–307
Fox, P.R., Trautwein, E.A., Hays, K.C., Bond, B.R., Sisson, D.D., and Moise, N.S. 1993, Comparision of taurine, a-tocopherol, retinol, selenium and total triglycerides and cholesterol concentrations in cats with cardiac disease and in healthy cats, Am. J. Vet. Res. 54:563–569.
Gabrielian, K., Wang, H.M., Ogden, T.E., and Ryan, S.J. 1992, In vitro stimulation of retinal pigment epithelium proliferation by taurine, Curr.Eye Res. 11:481–487.
Hayes, K.C., Garey, R.E., and Schmidt, S.Y. 1975, Retinal degeneration associated with taurine deficiency in the cat, Science 188:945–951.
Holmes R.P., Goodman H.O., Shihabi Z.K., Jarow, J.P., 1992 The taurine and hypotaurine content of human semen. J. Androl. 13: 289–292
Imaki, H., Jacobson, S.G., Kemp, C.M., Knighton, R. W., Neuringer, M., and Sturman, J. 1993, Retinal morphology and visual pigment levels in 6-and 12-month-old rhesus monkeys fed a taurine-free human infant formula, J. Neurosci. Res. 36:290–304.
Lake, N. 1989, The role of taurine in retinal function, in::“Extracellular and Intracellular Messengers in the Vertebrate Retina”, Redburn, D..A. and Pasantes-Morales, H. eds., Alan R.Liss, New York. pp.61–86.
Lake, N 1993 Loss of cardiac myofibrils: mechanism of contractile deficits induced by taurine deficiency. Am. J. Physiol. 264: 1323–1326.
Lehmann, A., Knutsson, L., and Bosaeus, I. 1990, Elevation of retinal levels and suppression of alanine aminotransferase activity in the liver of taurine-deficient kittens, J. Nutr. 120:1163–1167.
Lima L, Drujan B, Matus P., 1990, Spatial distribution of taurine in the teleost retina and its role in retinal tissue regeneration, in “Taurine: Functional Neurochemistry, Physiology and Cardiology”, Pasantes-Morales, H., Martin, D. L., Shain, W., Martin del Rio, R.,eds Wiley-Liss, Inc. New York, Vol. 351 pp 103–112.
Lima L, Matus P., Drujan B., 1993, Taurine-induced regeneration of goldfish retina in culture may involve a calcium-mediated mechanism. J. Neurochem. 60:2153–2158.
Nakashima T., Shima T., Mitsuyoshi H., Inaba K., Matsumoto N., Sakamoto Y. and Kashima K., 1996 Taurine in the Liver: The function of taurine conjugated with bile acids In: “Taurine 2: Basic and Clinical Aspects”, pp. 85–92 (Ed. by Huxtable R., Azuma J., Kuriyama K., Nakagawa M. & Baba A.). Plenum Press, New York & London.
Negoro S. and Hara H., 1992. The effect of taurine on the age-related decline of the immune response in mice: the restorative effect on the T cell proliferative response to costimulation with ionomycin and phorbol myristate acetate. Adv. Exp. Med. Biol. 315: 229–239.
Pasantes-Morales, H., Quesada, O., Carabez, A., and Huxtable, R.J. 1983, Effects of the taurine transport antagonist, guanidinoethane sulfonate and betta-alanine, on the morphology of the rat retina, J. Neurosci. Res. 9:135–143.
Pasantes-Morales, H., Queseda O., Moran, J., 1998. Taurine: An osmolyte in mammalian tissues In: “Taurine 3: Cellular and regulatory mechanisms”, pp. 209–217 (Ed. by Schaffer S., Lombardinin B., & Huxtable R.J.). Plenum Press, NY & London.
Petrosian A.M. and Haroutounian J.E., 1998. The role of taurine in osmotic, mechanical and chemical protection of the retinal rod outer segments. In: “Taurine: Basic and Clinical Aspects”, pp. 407–413 (Schaffer S., Lombardinin B., and Huxtable R.J. Eds). Plenum Publ. Corp., New York and London.
Petrosian A.M. and Haroutounian J.E., 1998. Whether connections between disks and cellular membrane in the retinal rod outer segments are channelized. In: Proceedings of the 14th Internat. Congress on Electron Mycroscopy, Cancun, Mexico, Eds. H.A. Calderon Benavides and M. Jose Yacaman. Inst. Phys Publ. Biol Sci., 7:249–250.
Petrosian A.M., Haroutounian J.E., Zueva L.V., 1996. Tauret: A taurine-related endogenous substance in the retina and its role in vision. In: “ Taurine: Basic and Clinical Aspects” (Ed. Huxtable R.J., Azuma J., Kuriyama K., Nakagawa M. & Baba A.) p.333–42. Plenum Publ. Corp, New York.
Petrosian A.M,. Haroutounian J.E.,. Gundersen T.E, Blomhoff R., Fugelli K. and Kanli H., 1999. Effects of osmotic and light stimulation on 3H-taurine efflux from isolated rod outer segments and synthesis of tauret in the frog retina. This volume.
Petrosian, A.M. and Haroutounian, J.E. 1988, On the possibility of retinal transportation in rods by tauret through special channels. 13th All-Union conf. on Electron Microscopy, (Abstract), Zvenigorod, Moscow.
Petrosian, A.M. and Haroutounian, J.E. 1990, Tauret: further studies of the role of taurine in retina, in: “Taurine: Functional Neurochemistry, Physiology, and Cardiology”, Pasantes-Morales, H., Martin, D.L., Shain, W. and del Rio, R.M. eds., Wiley-Liss, New York, pp.471–475.
Porter D.W., Kaczmarczyk W., Martin W.G., 1993. The effect of taurine on the incorporation of thymidin by chick B cells. Comp. Biochem. Physiol. B. 106:251–254.
Salceda, R., Lopez-Colome, A.M., and Pasantes-Morales, H. 1977, Light-stimulated release of 35S-taurine from frog retinal outer segments, Brain Res. 135:186–191.
Schalinske, K.L., Steele, R.D. 1991, !3-cis-Retinoic acid alters methionine metabolism in rats, J. Nutr. 121:1714–1719.
Schmidt, S.Y., Berson, E.L., Watson, G., and Huang, C. 1977, Retinal degeneration in cats fed casein. III.taurine deficiency and ERG amplitudes, Inves. Ophtal. Vis.Sci. 16:673–678.
Sivaprasadarao A. and Findlay J.B.C., 1994, The retinol-binding protein superfamily. In: Vitamin A in health and disease. Rune Blomhoff ed. Marcel Dekker, Inc, New York, Basel, Hong Kong pp 87–117.
Sturman, J..A. 1990, Taurine deficiency, in: “Taurine: Functional Neurochemistry, Physiology, and Cardiology”, Pasantes-Morales, H., Martin, D.L., Shain, W. and del Rio, R.M. eds., Wiley-Liss. New York, pp.385–395.
Sturman, J.A., Hayes K.C., 1980, The biology of taurine in nutrition and development. Adv. Nutr. Res., 3:231–299
Takanami M. and Miura K.L., 1963, Biochem. Biophys. Acta, 72:237–
Voaden, M. J., Lake, N., Marshall, J., Morjaria, B. 1977. Studies on the distribution of taurine and other neuroactive amino acids in the retina. Exp. Eye Res. 25:249–257.
Wake K., 1994, Role of perisinusoidal stellate cells in vitamin A storage. In: Vitamin A in health and disease. Rune Blomhoff ed. Marcel Dekker, Inc, New York, Basel, Hong Kong pp 73–86.
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Petrosian, A.M., Haroutounian, J.E., Gundersen, T.E., Blomhoff, R., Fugelli, K., Kanli, H. (2002). New HPLC Eevidence on Endogenous Tauret in Retina and Pigment Epithelium. In: Della Corte, L., Huxtable, R.J., Sgaragli, G., Tipton, K.F. (eds) Taurine 4. Advances in Experimental Medicine and Biology, vol 483. Springer, Boston, MA. https://doi.org/10.1007/0-306-46838-7_50
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DOI: https://doi.org/10.1007/0-306-46838-7_50
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