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Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

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Abstract

The existence of two cryptic species within strains of the wood-decaying fungus Fomes fomentarius was revealed recently based on the internal transcribed spacer (ITS) sequence variability. In this study for the first time the sequences of another molecular markers, partial translation elongation factor 1-α (efa) region and partial 25S large subunit ribosomal RNA gene were obtained and used to evaluate genetic variability of F. fomentarius. Congruent phylogeny was observed for all three markers used confirming the presence of two cryptic species within F. fomentarius. Surprisingly, ITS sequence variability within F. fomentarius was significantly lower compared to the variability of efa sequences (0.023 versus 0.036 nucleotide substitutions per site) questioning the discriminatory power of ITS sequences for fungal species identification.

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Abbreviations

efa :

translation elongation factor 1-α

ITS:

internal transcribed spacer

LSU:

25S large subunit ribosomal RNA

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Authors and Affiliations

  1. Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, SK 04001, Košice, Slovakia

    Peter Pristas

  2. Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, Tajovského 40, SK 97401, Banská Bystrica, Slovakia

    Peter Pristas, Svetlana Gaperova & Jana Judova

  3. Department of Biology and General Ecology, Faculty of Ecology and Environmental Sciences, Technical University, T. G. Masaryka 24, SK 96053, Zvolen, Slovakia

    Jan Gaper

  4. Department of Biology and Ecology, Faculty of Sciences, University of Ostrava, Chittussiho 10, CZ 71000, Ostrava, Czech Republic

    Jan Gaper

Authors
  1. Peter Pristas
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  2. Svetlana Gaperova
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  3. Jan Gaper
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  4. Jana Judova
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Correspondence to Peter Pristas.

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Pristas, P., Gaperova, S., Gaper, J. et al. Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences. Biologia 68, 816–820 (2013). https://doi.org/10.2478/s11756-013-0228-9

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  • DOI: https://doi.org/10.2478/s11756-013-0228-9

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