Abstract
We previously developed a separation-free ligase detection reaction assay based on fluorescence resonance energy transfer from a donor quantum dot to an acceptor fluorescent dye. This assay could successfully detect one cancer mutation among 10 wild-type templates. In the current study, the mutation-discrimination threshold was improved by one order of magnitude by replacing the original acceptor dye (Alexa Fluor 647) with another fluorescent dye (Cyanine 5) that was spectrally similar but more fluorescent.
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This work was supported by the Japan Society for the Promotion of Science's (JSPS) KAKENHI (Grant Number 13250199).
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Hagihara, K., Tsukagoshi, K., Nakajima, C. et al. Improvement of the Mutation-Discrimination Threshold for Rare Point Mutations by a Separation-Free Ligase Detection Reaction Assay Based on Fluorescence Resonance Energy Transfer. ANAL. SCI. 32, 367–370 (2016). https://doi.org/10.2116/analsci.32.367
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DOI: https://doi.org/10.2116/analsci.32.367