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Quantitative Determination of Cyclosporine in Human Whole Blood by Ultra-Performance Liquid Chromatography with Triple Quadrupole Tandem Mass Spectrometry

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Abstract

Cyclosporine is an immunosuppressant drug used in organ transplants or for the treatment of autoimmune diseases. We developed and validated a simple, sensitive, and specific method using UPLC-MS/MS to determine cyclosporine levels in human whole blood. MS/MS detection was performed in the positive electrospray ionization mode with multiple reaction monitoring. Cyclosporine was extracted from whole-blood samples using ascomycin as an internal standard. The mass transitions m/z 1203.49 → 1185.53 and m/z 814.71 → 796.67 were used to assay the analyte and IS. This method was validated with respect to linearity, specificity, accuracy, precision, recovery, and stability. The method exhibited a linear response from 10 to 1000 ng mL-1 with correlation coefficient values >0.99. The precision and the accuracy values were within 15%, except at the lower limit of quatification (LLOQ). Cyclosporine was stable in whole blood with no evidence of degradation. This method was successfully applied to a pharmacokinetic study of cyclosporine in healthy volunteers following oral administration.

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Authors and Affiliations

  1. Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital, 130 Dongdeok-ro, Jung-gu, Daegu, 700-721, Korea

    Hyun Jin Jung, Mi-Ri Gwon, Jeonghyeon Park, Jeong Ju Seo, Sook Jin Seong, Hae Won Lee & Young-Ran Yoon

  2. KNU Bio-Medical Convergence Program for Creative Talent, Kyungpook National University Graduate School, 680 Gukchaebosang-ro, Jung-gu, Daegu, 700-842, Korea

    Mi-Ri Gwon & Young-Ran Yoon

  3. School of Nursing, Yeungnam College of Science & Technology, 170 Hyeongchung-ro, Nam-gu, Daegu, 705-703, Korea

    Eun Hee Kim

  4. College of Nursing, Kyungpook National University, 680 Gukchabosang-ro, Jung-gu, Daegu, 700-422, Korea

    Soon Rim Suh

  5. Division of Endocrinology and Metabolism, Department of Internal Medicine, Kyungpook National University School of Medicine, Jung-gu, Daegu, 700-842, Korea

    Ji Yun Jeong

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  1. Hyun Jin Jung
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  2. Mi-Ri Gwon
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  7. Soon Rim Suh
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  8. Ji Yun Jeong
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  9. Hae Won Lee
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Corresponding authors

Correspondence to Hae Won Lee or Young-Ran Yoon.

Additional information

H. J. J. and M.-R. G. equally contributed to this work.

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Jung, H.J., Gwon, MR., Park, J. et al. Quantitative Determination of Cyclosporine in Human Whole Blood by Ultra-Performance Liquid Chromatography with Triple Quadrupole Tandem Mass Spectrometry. ANAL. SCI. 30, 293–298 (2014). https://doi.org/10.2116/analsci.30.293

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  • DOI: https://doi.org/10.2116/analsci.30.293

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