Abstract
A carbon nanofiber-based luminol-biotin probe was synthesized for the sensitive chemiluminescence (CL) detection of a target protein by grafting luminol and biotin onto an oxidized carbon nanofiber. This carbon nanofiber was prepared by chemical vapor-deposition with methane in the presence of the Ni–Cu–MgO catalyst, which was followed by oxidization with HNO3–H2SO4 to produce a carboxyl group on the surface of the nanofiber. The material was grafted with luminol and biotin by means of a standard carbodiimide activation of COOH groups to produce corresponding amides. The substance was water-soluble and thus could be utilized as a sensitive CL probe for a protein assay. The probe showed highly specific affinity towards the biotin-labeled antibody via a streptavidin–biotin interaction. The detection limit for this model assay was approximately 0.2 pmol of the biotinized IgG spotted on a polyvinylidene fluoride (PVDF) membrane. Nonspecific binding to other proteins was not observed. Therefore, the synthesized carbon nanofiber-based CL probe may be useful for a sensitive and specific analysis of the target protein.
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References
J. Wang and Y. Lin, TrAC, Trends Anal. Chem., 2008, 27, 619.
J. Huang, Y. Liu, and T. You, Anal. Methods, 2010, 2, 202.
L. Wu, F. Yan, and H. Ju, J. Immunol. Methods, 2007, 322, 12.
L. Ding, C. Hao, X. Zhang, and H. Ju, Electrochem. Commun., 2009, 11, 760.
P. Scrimin and L. J. Prins, Chem. Soc. Rev., 2011, 40, 4488.
S. K. Sia, V. Linder, B. A. Parviz, A. Siegel, and G. M. Whitesides, Angew. Chem., Int. Ed. Engl., 2004, 43, 498.
M. Zhou, J. Roovers, G. P. Robertson, and C. P. Grover, Anal. Chem., 2003, 75, 6708.
Q. Zhang and L.-H. Guo, Bioconjugate Chem., 2007, 18, 1668.
J. Lei and H. Ju, Chem. Soc. Rev., 2012, 41, 2122.
R. J. Amir, E. Danieli, and D. Shabat, Chem. Eur. J., 2007, 13, 812.
H. Zhang, C. Smanmoo, T. Kabashima, J. Lu, and M. Kai, Angew. Chem., Int. Ed. Engl., 2007, 46, 8226.
G. Lai, F. Yan, and H. Ju, Anal. Chem., 2009, 81, 9730.
C. M. Soto, A. S. Blum, G. J. Vora, N. Lebedev, C. E. Meador, A. P. Won, A. Chatterji, J. E. Johnson, and B. R. Ratna, J. Am. Chem. Soc., 2006, 128, 5184.
S. Bi, H. Zhou, and S. Zhang, Biosens. Bioelectron., 2009, 24, 2961.
C. P. Chan, Y. Bruemmel, M. Seydack, K. Sin, L. Wong, E. Merisko-Liversidge, D. Trau, and R. Renneberg, Anal. Chem., 2004, 76, 3638.
R. Genç, D. Murphy, A. Fragoso, M. Ortiz, and C. K. O’Sullivan, Anal. Chem., 2011, 83, 563.
Y. Song, W. Wei, and X. Qu, Adv. Mater., 2011, 23, 4215.
T. Krawczyk, M. Kondo, M. G. Azam, H. Zhang, T. Shibata, and M. Kai, Analyst, 2010, 135, 2894.
G. Azam, T. Shibata, T. Kabashima, and M. Kai, Anal. Sci., 2011, 27, 715.
M. G. Azam, T. Shibata, T. Kabashima, and M. Kai, Anal. Bioanal. Chem., 2011, 401, 1211.
H. Zhang, T. Shibata, T. Krawczyk, T. Kabashima, J. Lu, M. K. Lee, and M. Kai, Talanta, 2009, 79, 700.
C. A. Marquette, P. Hezard, A. Degiuli, and L. J. Blum, Sens. Actuators, B, 2006, 113, 664.
C. L. Cooper, P. L. Dubin, A. B. Kayitmazer, and S. Turksen, Curr. Opin. Colloid Interface Sci., 2005, 10, 52.
A. Roda, M. Guarigli, E. Michelini, M. Mirasoli, and P. Pasini, Anal. Chem., 2003, 75, 463A.
H. Wang and R. T. K. Baker, J. Phys. Chem. B, 2004, 108, 20273.
S. Chen, X. Zhao, J. Chen, J. Chen, L. Kuznetsova, S. S. Wong, and I. Ojima, Bioconjugate Chem., 2010, 21, 979.
N. M. Green, Methods Enzymol., 1970, 18, 418.
E. P. Diamandis and T. K. Christopoulos, Clin. Chem., 1991, 37, 625.
H. Karatani, Bull. Chem. Soc. Jpn., 1987, 60, 2023.
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Baj, S., Krawczyk, T., Pradel, N. et al. Carbon Nanofiber-based Luminol-biotin Probe for Sensitive Chemiluminescence Detection of Protein. ANAL. SCI. 30, 1051–1056 (2014). https://doi.org/10.2116/analsci.30.1051
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DOI: https://doi.org/10.2116/analsci.30.1051