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Light Scattering: a Novel Approach to Analyze Protein 4.1R FERM Domain Interaction with Inside-out-vesicles in Solution

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Abstract

In this study, we describe a novel application for light scattering, a method widely used for separation of molecules in solution based on their size. We demonstrate that light scattering analysis can monitor the change in particle size of protein 4.1R prior to and after binding to red blood cell inside-out-vesicles in solution. Light scattering constitutes therefore a novel tool to analyze protein-binding association constants.

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Abbreviations

IOVs:

inside-out-vesicles

SDS-PAGE:

SDS-poly-acrylamide gel electrophoresis

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Author information

Author notes

  1. K. S. and W. N. contributed equally to this work.

Authors and Affiliations

  1. Sysmex Corporation, 4-4-4 Takatsukadai, 651-2271, Nishi, Kobe, Japan

    Kohei Shiba

  2. Center for Geo-Environmental Science, Graduate School of Engineering and Resource Science, Akita University, 1-1 Tegata-Gakuén, 010-8502, Akita, Japan

    Wataru Nunomura

  3. Department of Life Science, Graduate School of Engineering and Resource Science, Akita University, 1-1 Tegata-Gakuén, 010-8502, Akita, Japan

    Wataru Nunomura

  4. Department of Biochemistry, Tokyo Women’s Medical University, 8-1 Kawada, 162-8666, Shinjuku, Tokyo, Japan

    Yuichi Takakuwa

Authors
  1. Kohei Shiba
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  2. Wataru Nunomura
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  3. Yuichi Takakuwa
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Corresponding author

Correspondence to Kohei Shiba.

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Shiba, K., Nunomura, W. & Takakuwa, Y. Light Scattering: a Novel Approach to Analyze Protein 4.1R FERM Domain Interaction with Inside-out-vesicles in Solution. ANAL. SCI. 28, 613–615 (2012). https://doi.org/10.2116/analsci.28.613

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  • DOI: https://doi.org/10.2116/analsci.28.613

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