Abstract
Spherical molecularly imprinted polymer particles for bisphenol A (BPA-MIP) were easily prepared by using a Y-junction microfluidic device. The sizes of the obtained BPA-MIP particles were found to be 86 μm with a narrow size distribution. The binding characteristics were investigated by drawing a binding isotherm to estimate the binding constant and by switching the polarity of solvents to examine the feasibility of use as a medium for affinity chromatography. When dichloromethane was used as a solvent, BPA was strongly bound to the spherical BPA-MIP particles based on hydrogen bond formation; after switching the solvent to methanol, BPA was eluted quantitatively due to the weakening of the hydrogen bonding, suggesting that the spherical BPA-MIP particles can be applied to affinity-type solid-phase extraction for BPA. As the present method can provide a diverse range of spherical MIPs without tedious procedures, MIP-based affinity media will be able to be more readily used as pretreatment and/or purification for various fields.
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References
J. S. Fritz, “Analytical Solid-phase Extraction”, 1999, Wiley-VCH, Weinheim.
M.-C. Hennion, J. Chromatogr., A, 1999, 856, 3.
E. M. Thurman and M. S. Mills, “Solid-phase Extraction: Principles and Practice”, 1998, Wiley, New York.
L. I. Andersson, J. Chromatogr., B, 2000, 739, 163.
J. Haginaka, J. Sep. Sci., 2009, 32, 1548.
G. Wulff, Angew. Chem., Int. Ed. Engl., 1995, 34, 1812.
M. Komiyama, T. Takeuchi, T. Mukawa, and H. Asanuma, “Molecular Imprinting”, 2002, Wiley-VCH, Weinheim.
K. Haupt and K. Mosbach, Chem. Rev., 2000, 100, 2495.
R. A. Anderson, M. M. Ariffin, P. A. G. Cormack, and E. I. Miller, Forensic Sci. Int., 2008, 174, 40.
Y. Zhan, Y. Li, Y. Hu, G. Li, and Y. Chen, J. Chromatogr., A, 2010, 1217, 7337.
J. Matsui, M. Okada, M. Tsuruoka, and T. Takeuchi, Anal. Commun., 1997, 34, 85.
J. Matsui, K. Fujiwara, S. Ugata, and T. Takeuchi, J. Chromatogr., A, 2000, 889, 25.
J.-P. Lai, X.-Y. Lu, C.-Y. Lu, H.-F. Ju, and X.-W. He, Anal. Chim. Acta, 2001, 442, 105.
J.-P. Lai, R. Niessner, and D. Knopp, Anal. Chim. Acta, 2004, 522, 137.
R. Walsh, Q. Osmani, H. Hughes, P. Duggan, and P. McLoughlin, J. Chromatogr., B, 2011, 879, 3523.
A. Kawai, S. Matsumoto, H. Kiriya, T. Oikawa, K. Hara, T. Ohkawa, T. Futami, K. Katayama, and K. Nishizawa, TOSOH Research and Technology Review, 2003, 47, 3.
S. Okushima, T. Nisisako, T. Torii, and T. Higuchi, Langmuir, 2004, 20, 9905.
M. L. J. Steegmans, K. G. P. H. Schroën, and R. M. Boom, Langmuir, 2009, 25, 3396.
Z. Nie, S. Xu, M. Seo, P. C. Lewis, and E. Kumacheva, J. Am. Chem. Soc, 2005, 127, 8058.
S.-H. Kim, A. Abbaspourrad, and D. A. Weitz, J. Am. Chem. Soc., 2011, 133, 5516.
A. Kubo, H. Shinmori, and T. Takeuchi, Chem. Lett., 2006, 35, 588.
S. Kitamura, T. Suzuki, S. Sanoh, R. Kohta, N. Jinno, K. Sugihara, S. Yoshihara, N. Fujimoto, H. Watanabe, and S. Ohta, Toxicol. Sci., 2005, 84, 249.
T. Ikegami, W.-S. Lee, H. Nariai, and T. Takeuchi, Anal. Bioanal. Chem., 2004, 379, 1898.
T. Ikegami, W.-S. Lee, H. Nariai, and T. Takeuchi, J. Chromatogr., B, 2004, 804, 197.
T. Ikegami, T. Mukawa, H. Nariai, and T. Takeuchi, Anal. Chim. Acta, 2004, 504, 131.
S. Sasaki, T. Ooya, and T. Takeuchi, Polym. Chem., 2010, 1, 1684.
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Takano, E., Tanaka, F., Ooya, T. et al. Molecularly Imprinted Microspheres for Bisphenol A Prepared Using a Microfluidic Device. ANAL. SCI. 28, 457–461 (2012). https://doi.org/10.2116/analsci.28.457
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DOI: https://doi.org/10.2116/analsci.28.457