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Development of a N-Acetyl-β-d-glucosaminidase (NAG) Assay on a Centrifugal Lab-on-a-compact-disc (Lab-CD) Platform

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Abstract

A centrifugal microfluidic platform, which is also known as lab-on-a-compact-disc (Lab-CD), was developed for use as a urinary N-acetyl-β-d-glucosaminidase (NAG) activity assay. In this work, Lab-CD design, centrifugal operations and analytical procedures were established. Automated liquid handling on Lab-CD processes, such as fluid transport, sample metering, mixing, and fluorescence detection are accomplished using a portable Lab-CD system. The linearity of the NAG assay using 4-methylumbelliferyl-N-acetyl-β-d-glucosaminide (4-MU-GlcNAc) was found to be acceptable in the range of 2.5 to 20 U L−1; the relative standard deviations for the fluorescence intensity of eight samples (7.5 U L−1) was 6.4%. Clinical diagnostics is one of the most promising applications for Lab-CD technologies. All the benefits of miniaturization, such as reduced sample requirement, reduced reagent consumption and automation, are realized in this investigation.

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Authors and Affiliations

  1. Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, 563-8577, Ikeda, Osaka, Japan

    Yoshihide Tanaka

  2. Faculty of Pharmaceutical Sciences, Kinki University, 3-4-1 Kowakae, 577-8502, Higashi-osaka, Osaka, Japan

    Seira Okuda, Ayumi Sawai & Shigeo Suzuki

Authors
  1. Yoshihide Tanaka
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  2. Seira Okuda
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  3. Ayumi Sawai
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  4. Shigeo Suzuki
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Correspondence to Yoshihide Tanaka.

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Tanaka, Y., Okuda, S., Sawai, A. et al. Development of a N-Acetyl-β-d-glucosaminidase (NAG) Assay on a Centrifugal Lab-on-a-compact-disc (Lab-CD) Platform. ANAL. SCI. 28, 33–38 (2012). https://doi.org/10.2116/analsci.28.33

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  • DOI: https://doi.org/10.2116/analsci.28.33

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