Abstract
In order to elucidate the role of desorption/ionization efficiency of peptides in MALDI-MS, we focused on peptides with disulfide bonds, which form a rigid tertiary structure. We synthesized seven sets of peptides with one disulfide bond (oxytocin, somatostatin, [Arg8]-vasopressin, [Arg8]-vasotocin, cortistatin, melanin-concentrating hormone, urotensin II-related peptide) and five sets of peptides with two disulfide bonds (tertiapin, α-conotoxin GI, α-conotoxin ImI, α-conotoxin MI and α-conotoxin SI). Each peptide set consisted of three peptides: the oxidized form (S-S type), the reduced form (SH type), and an internal standard peptide in which all cysteine residues were substituted with alanine residues. In the case of urotensin II-related peptide, tertiapin, α-conotoxin ImI and α-conotoxin MI, the reduced form showed higher desorption/ionization efficiency than the oxidized form. In contrast, the other peptides revealed higher desorption/ionization efficiency in the oxidized form relative to the reduced form. These results imply that a rigid structure of peptides formed by disulfide bonds does not correlate with desorption/ionization efficiency in MALDI-MS.
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References
K. Dreisewerd, Chem. Rev., 2003, 103, 395.
Y. Shigeri, S. Fujii, R. Obana, Y. Hagihara, M. Jitosho, H. Kawasaki, and R. Arakawa, J. Mass Spectrom., 2009, 44, 1119.
H. Wenschuh, P. Halada, S. Lamer, P. Jungblut, and E. Krause, Rapid Commun. Mass Spectrom., 1998, 12, 115.
L. Mayr, D. Willbold, O. Landt, and F. Schmid, Protein Sci., 1994, 3, 227.
M. Feige and L. Hendershot, Curr. Opin. Cell Biol., 2011, 23, 167.
K. Inaba, Genes Cells, 2010, 15, 935.
H. Yang, N. Liu, X. Qiu, and S. Liu, J. Am. Soc. Mass Specrtom., 2009, 20, 2284.
S. Cohen and B. Chait, Anal. Chem., 1996, 68, 31.
O. Vorm, P. Roepstorff, and M. Mann, Anal. Chem., 1994, 66, 3281.
R. Kratzer, C. Eckerskorn, M. Karas, and F. Lottspeich, Electrophoresis, 1998, 19, 1910.
Z. Olumee, M. Sadeghi, X. Tang, and A. Vertes, Rapid Commun. Mass Spectrom., 1995, 9, 744.
S. Baumgart, Y. Lindner, R. Kuhne, A. Oberemm, H. Wenschuh, and E. Krause, Rapid Commun. Mass Spectrom., 2004, 18, 863.
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Shigeri, Y., Inazumi, S., Hagihara, Y. et al. Desorption/Ionization Efficiency of Peptides Containing Disulfide Bonds in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. ANAL. SCI. 28, 295–299 (2012). https://doi.org/10.2116/analsci.28.295
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DOI: https://doi.org/10.2116/analsci.28.295