Abstract
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of a model peptide, Substance P (SubP), was carried out using 2,4,6-trihydroxyacetophenone (THAP) and 2,4-dihydroxyacetophenone (DHAP) with cyclodextrins (cyclodextrin-supported matrix). It was found that the use of a cyclodextrin-supported matrix simplified the mass spectrum in the low-molecular-weight region. The interaction between THAP/DHAP and cyclodextrin (CD) was studied by UV-vis absorption spectroscopy and the incorporation of matrix molecules into the cyclodextrin cavity was confirmed by 1H-NMR spectroscopy. DHAP showed tight incorporation with βCD (βCD(DHAP)) rather than THAP and it was found that the matrix-related peaks could be weakened by less than one third of the peak intensity of a protonated analyte. The βCD(DHAP) matrix was applied to the measurements of two low-molecular-weight compounds; adenosine and adrenaline. It became clear that the cyclic structure of the CD and the host-guest interaction between βCD and the matrix molecule were important to reduce the matrix-related peaks of THAP and DHAP.
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F. Hillenkamp, M. Karas, R. C. Beavis, and B. T. Chait, Anal. Chem., 1991, 63, 1193A.
F. Hillenkamp and M. Karas, Int. J. Mass. Spectrom., 2000, 200, 71.
C. Koester, J. A. Castoro, and C. L. Wilkins, J. Am. Chem. Soc., 1992, 114, 7572.
J. Asara and J. Allison, J. Am. Soc. Mass Spectrom., 1999, 10, 35.
X. Yang, H. Wu, T. Kobayashi, R. J. Solaro, and R. B. van Breemen, Anal. Chem., 2004, 76, 1532.
S. Kjellstroem and O. N. Jensen, Anal. Chem., 2004, 76, 5109.
T. Nishikaze and M. Takayama, Rapid Commun. Mass Spectrom., 2007, 21, 3345.
T. Kobayashi, H. Kawai, T. Suzuki, T. Kawanishi, and T. Hayakawa, Rapid Commun. Mass Spectrom., 2004, 18, 1156.
R. Arakawa, Y. Shimomae, H. Morikawa, K. Ohara, and S. Okuno, J. Mass Spectrom., 2004, 39, 961.
H. Kawasaki, T. Yonezawa, T. Watanabe, and R. Arakawa, J. Phys. Chem. C, 2007, 111, 16278.
Y. Komori, H. Shima, T. Fujino, J. N. Kondo, K. Hashimoto, and T. Korenaga, J. Phys. Chem. C, 2010, 114, 1593.
S. Yamaguchi, T. Fujita, T. Fujino, and T. Korenaga, Anal. Sci., 2008, 24, 1497.
A. Meyer, N. Spinelli, J.-L. Imbach, and J.-J. Vasseur, Rapid Commun. Mass Spectrom., 2000, 14, 234.
M. Knochenmuss, F. Dubois, M. J. Dale, and R. Zenobi, Rapid Commun. Mass Spectrom., 1996, 10, 871.
Y. Yamamoto and Y. Inoue, J. Carbohydr. Chem., 1989, 8, 29.
J. T. Edward, J. Chem. Educ., 1970, 47, 261.
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Fujita, T., Fujino, T., Hirabayashi, K. et al. MALDI Mass Spectrometry Using 2,4,6-Trihydroxyacetophenone and 2,4-Dihydroxyacetophenone with Cyclodextrins: Suppression of Matrix-related Ions in Low-molecular-weight Region. ANAL. SCI. 26, 743–748 (2010). https://doi.org/10.2116/analsci.26.743
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DOI: https://doi.org/10.2116/analsci.26.743