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Aptamer-Mediated Chemiluminescence Detection of Prion Protein on a Membrane Using Trimethoxyphenylglyoxal

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Abstract

Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt–Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot–1.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan

    Towhid Hossain, Takayuki Shibata, Tsutomu Kabashima & Masaaki Kai

  2. Global Center of Excellence Program, Nagasaki University, Nagasaki, 852-8521, Japan

    Masaaki Kai

Authors
  1. Towhid Hossain
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  2. Takayuki Shibata
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  3. Tsutomu Kabashima
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  4. Masaaki Kai
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Corresponding author

Correspondence to Masaaki Kai.

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Hossain, T., Shibata, T., Kabashima, T. et al. Aptamer-Mediated Chemiluminescence Detection of Prion Protein on a Membrane Using Trimethoxyphenylglyoxal. ANAL. SCI. 26, 645–647 (2010). https://doi.org/10.2116/analsci.26.645

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  • DOI: https://doi.org/10.2116/analsci.26.645

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