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Measurement of Benzophenones in Human Urine Samples by Stir Bar Sorptive Extraction and Thermal Desorption-Gas Chromatography–Mass Spectrometry

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Abstract

Determination of benzophenones (BPs) in human urine samples by stir bar sorptive extraction (SBSE) and thermal desorption (TD)-gas chromatography–mass spectrometry (GC-MS) is described. As analytes, BP, its metabolites benzhydrol (BP-OH) and 2-hydroxybenzophenone (2OH-BP), and its derivatives 2-hydroxy-4-methoxybenzophenone (BP-3) and 2-hydroxy-4-methoxy-4′-methylbenzophenone (BP-10) were selected. After enzymatic hydrolysis, a polydimethylsiloxane (PDMS) stir bar was placed in a urine sample diluted 1:1 with water and stirred for 60 min at room temperature. The limit of quantification (LOQ) of BPs is 0.2–0.5 ng ml–1 (ppb). The method showed linearity over the calibration range (0.2–10 or 0.5–10 ng ml–1), and the correlation coefficients were equal to or higher than 0.993 for all of the analytes. The average recoveries of BPs were equal to or higher than 98.7% (RSD: 1.5–4.8%, n = 6).

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References

  1. D. Miller, B. B. Wheals, N. Beresford, and J. P. Sumpter, Environ. Health Perspect., 2001, 109, 133.

    CAS  PubMed  PubMed Central  Google Scholar 

  2. M. Schlumpf, B. Cotton, M. Conscience, V. Haller, B. Steinmann, and W. Lichtensteiger, Environ. Health Perspect., 2001, 109, 239.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. S. Takatori, Y. Kitagawa, H. Oda, G. Miwa, J. Nishikawa, T. Nishihara, H. Nakazawa, and S. Hori, J. Health Sci., 2003, 49, 91.

    Article  CAS  Google Scholar 

  4. C. Nerín and E. Asensio, Anal. Bioanal. Chem., 2007, 389, 589.

    Article  PubMed  Google Scholar 

  5. L. Castle, C. P. Offen, M. J. Baxter, and J. Gilbert, Food Addit. Contam., 1997, 14, 35.

    Article  CAS  PubMed  Google Scholar 

  6. Y. Nakagawa, T. Suzuki, and S. Tayama, Toxicology, 2000, 156, 27.

    Article  CAS  PubMed  Google Scholar 

  7. V. Sarveiya, S. Risk, and H. A. E. Benson, J. Chromatogr., B, 2004, 803, 225.

    Article  CAS  Google Scholar 

  8. X. Ye, Z. Kuklenyik, L. L. Needham, and A. M. Calafat, Anal. Chem., 2005, 77, 5407.

    Article  CAS  PubMed  Google Scholar 

  9. X. Ye, Z. Kuklenyik, L. L. Needham, and A. M. Calafat, Anal. Bioanal. Chem., 2005, 383, 638.

    Article  CAS  PubMed  Google Scholar 

  10. T. Felix, B. J. Hall, and J. S. Brodbelt, Anal. Chim. Acta, 1998, 371, 195.

    Article  CAS  Google Scholar 

  11. E. Baltussen, P. Sandra, F. David, and C. Cramers, J. Microcol. Sep., 1999, 11, 737.

    Article  CAS  Google Scholar 

  12. M. Kawaguchi, R. Ito, K. Saito, and H. Nakazawa, J. Pharm. Biomed. Anal., 2006, 40, 500.

    Article  CAS  PubMed  Google Scholar 

  13. R. Rodil and M. Moeder, J. Chromatogr., A, 2008, 1179, 81.

    Article  CAS  PubMed  Google Scholar 

  14. M. Kawaguchi, R. Ito, N. Endo, N. Sakui, N. Okanouchi, K. Saito, N. Sato, T. Shiozaki, and H. Nakazawa, Anal. Chim. Acta, 2006, 557, 272.

    Article  CAS  Google Scholar 

  15. M. Kawaguchi, R. Ito, H. Honda, N. Endo, N. Okanouchi, K. Saito, Y. Seto, and H. Nakazawa, J. Chromatogr., A, 2008, 1200, 260.

    Article  CAS  PubMed  Google Scholar 

  16. N. Okanouchi, H. Honda, R. Ito, M. Kawaguchi, K. Saito, and H. Nakazawa, Anal. Sci., 2008, 24, 627.

    Article  CAS  PubMed  Google Scholar 

Download references

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Author notes

  1. Migaku Kawaguchi

    Present address: Bio-Medical Standard Section, National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), 1–1–1 Umezono, Tsukuba, Ibaraki, 305-8563, Japan

Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2–4–41 Ebara, Shinagawa, Tokyo, 142-8501, Japan

    Migaku Kawaguchi, Rie Ito, Hidehiro Honda, Naoyuki Endo, Noriya Okanouchi, Koichi Saito & Hiroyuki Nakazawa

  2. National Research Institute of Police Science, 6–3–1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan

    Yasuo Seto

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  1. Migaku Kawaguchi
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  2. Rie Ito
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  3. Hidehiro Honda
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  4. Naoyuki Endo
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  5. Noriya Okanouchi
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  6. Koichi Saito
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  7. Yasuo Seto
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  8. Hiroyuki Nakazawa
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Corresponding author

Correspondence to Hiroyuki Nakazawa.

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Kawaguchi, M., Ito, R., Honda, H. et al. Measurement of Benzophenones in Human Urine Samples by Stir Bar Sorptive Extraction and Thermal Desorption-Gas Chromatography–Mass Spectrometry. ANAL. SCI. 24, 1509–1512 (2008). https://doi.org/10.2116/analsci.24.1509

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  • DOI: https://doi.org/10.2116/analsci.24.1509

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