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Drug Response Assay System in a Microchip Using Human Hepatoma Cells

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Abstract

A microchip-based cell response assay system to an anticancer agent was developed. The hepatoma cell line HepG2 was used to assess the effects of an anticancer agent, doxorubicin. The required cell number was reduced by two orders, and the observation of the time course of cell response became possible. The system clearly showed that treatment with higher doses of the drug or longer exposure times gave more effects to cells. The possibilities of novel drug response studies or toxicity assay system were demonstrated.

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Authors and Affiliations

  1. Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Yuki Tanaka & Takehiko Kitamori

  2. Core Research for Evolutional Scienceand Technology (CREST), Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Yuki Tanaka, Kiichi Sato, Masayuki Yamato, Teruo Okano & Takehiko Kitamori

  3. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-8657, Japan

    Kiichi Sato

  4. Micro Chemistry Group, Special Research Laboratory for Optical Science, Kanagawa Academy of Science and Technology, 3-2-1 Sakado, Takatsu, Kawasaki, 213-0012, Japan

    Kiichi Sato & Takehiko Kitamori

  5. Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo, 162-8666, Japan

    Masayuki Yamato & Teruo Okano

Authors
  1. Yuki Tanaka
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  2. Kiichi Sato
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  3. Masayuki Yamato
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  4. Teruo Okano
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  5. Takehiko Kitamori
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Corresponding author

Correspondence to Takehiko Kitamori.

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Tanaka, Y., Sato, K., Yamato, M. et al. Drug Response Assay System in a Microchip Using Human Hepatoma Cells. ANAL. SCI. 20, 411–413 (2004). https://doi.org/10.2116/analsci.20.411

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  • DOI: https://doi.org/10.2116/analsci.20.411

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