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DNA-Conjugated Polymers for Self-Assembled DNA Chip Fabrication

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Abstract

We developed two DNA-conjugated polymers, one based on polyallylamine and the other on polyacrylic acid, for use in DNA chips. A 30-mer single-stranded DNA probe and thioctic acid were covalently attached to polyallylamine as sidechains. The same single-stranded DNA and 3-(pyridyldithio)propionyl hydrazide were covalently attached to polyacrylic acid as sidechains. Both DNA-conjugated polymers could be specifically immobilized onto a gold sensor substrate by a self-assembly technique. The interactions between fully matched DNA and each DNA-conjugated polymer were investigated by surface plasmon resonance. A gold surface modified with either DNA-conjugated polymer recognized fully matched DNA much better than unmatched DNA. The hybridization selectivity and efficiency of DNA-conjugated polyallylamine was optimized by adjusting the pH so as to reduce the effects of cationic polymer sidechains. The hybridization selectivity and efficiency of DNA-conjugated polymers were higher than those of a conventional immobilized thiol-based DNA. The coating of DNA-conjugated polymers reduced nonspecific adsorption of DNA by the gold substrate. DNA-conjugated polyacrylic acid was more selective toward fully matched DNA than was DNA-conjugated polyallylamine. Therefore, DNA-conjugated polymers show promise for application in novel DNA chips.

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Authors and Affiliations

  1. Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, 305-8562, Japan

    Shu Taira

  2. School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatsunokuchi, Ishikawa, 923-1292, Japan

    Kenji Yokoyama

Authors
  1. Shu Taira
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  2. Kenji Yokoyama
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Correspondence to Kenji Yokoyama.

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Taira, S., Yokoyama, K. DNA-Conjugated Polymers for Self-Assembled DNA Chip Fabrication. ANAL. SCI. 20, 267–271 (2004). https://doi.org/10.2116/analsci.20.267

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  • DOI: https://doi.org/10.2116/analsci.20.267

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