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Application of 4-(4-Nitrophenyl)-1,2,4-triazoline-3,5-dione to Analysis of 25- Hydroxyvitamin D3 in Human Plasma by Liquid Chromatography/Electron Capture Atmospheric Pressure Chemical Ionization-Mass Spectrometry

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Abstract

The utility of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-dione (NPTAD) as a derivatization reagent in the analysis of 25- hydroxyvitamin D3 [25(OH)D3] using liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry (LC/ECAPCI-MS) was examined. The derivatives of 25(OH)D3 with NPTAD underwent electron capture in the APCI source in the negative-ion mode and provided 30-fold higher sensitivity compared to an intact compound. This derivatization-LC/ECAPCI-MS method was applied to a plasma 25-hydroxyvitamin D3 assay, and gave satisfactory results in sensitivity, specificity, measurable range and throughput of the analysis.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-0934, Japan

    Tatsuya Higashi, Akinori Yamauchi & Kazutake Shimada

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  1. Tatsuya Higashi
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  2. Akinori Yamauchi
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  3. Kazutake Shimada
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Correspondence to Tatsuya Higashi.

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Higashi, T., Yamauchi, A. & Shimada, K. Application of 4-(4-Nitrophenyl)-1,2,4-triazoline-3,5-dione to Analysis of 25- Hydroxyvitamin D3 in Human Plasma by Liquid Chromatography/Electron Capture Atmospheric Pressure Chemical Ionization-Mass Spectrometry. ANAL. SCI. 19, 941–943 (2003). https://doi.org/10.2116/analsci.19.941

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  • DOI: https://doi.org/10.2116/analsci.19.941

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