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Determination of Serum 25-Hydroxyvitamin D3 by LC/MS/MS and Its Monthly Variation in Sapporo Indoor Workers

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Abstract

25-Hydroxyvitamin D3 (25(OH)D3) as the metabolite of vitamin D, is connected with various of diseases, and important to people with limited sunshine. Thus, the investigation of serum 25-hydroxyvitamin D and its variation in these people is necessary. In this study, a simple, precise, and accurate method for serum 25(OH)D3 determination by LC/MS/MS was developed. Serum samples were obtained monthly for one year from 11 male and 11 female indoor workers in Sapporo, Japan, and the overall 25(OH)D3 concentration was 12.9 ± 4.7 ng/mL. The 25(OH)D3 in females was significantly lower than that in males (14.0 ± 5.0 vs. 11.9 ± 4.3 ng/mL). The serum 25(OH)D3 concentration in males and females were both strongly correlated to UV-B radiation (r2 = 0.8477 and 0.7384, respectively), with a two-month's lag. Also the monthly change in 25(OH)D3 in males was more significant than that in females.

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Acknowledgments

This study was financially supported by the Regional Innovation Strategy Support Program, Sapporo Health Innovation “Smart-H”, of the Ministry of Education, Culture, Sports, Science and Technology, Japan, and partially supported by the Center of Innovation Program from Japan Science and Technology Agency, JST, by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science, Japan.

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Authors and Affiliations

  1. Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Sapporo, 060-0812, Japan

    Hiroaki Okabe, Maiko Yamamoto, Zhen Chen & Shu-Ping Hui

  2. Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Kita-14, Nishi-5, Sapporo, 060-8648, Japan

    Chikara Shimizu, Rei Kikuchi & Akiko Minami

  3. Graduate School of Information Science and Technology, Hokkaido University, Kita-14, Nishi-9, Sapporo, 060-0814, Japan

    Yi-Fan Chen, Hideyuki Imai & Masahiro Mizuta

  4. Department of Nutrition, Sapporo University of Health Sciences, 4-2-1-15 Nakanuma Nishi, Higashi, Sapporo, 007-0894, Japan

    Hitoshi Chiba

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  1. Hiroaki Okabe
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  2. Chikara Shimizu
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  3. Maiko Yamamoto
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  4. Rei Kikuchi
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  5. Akiko Minami
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  6. Yi-Fan Chen
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  7. Hideyuki Imai
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  8. Masahiro Mizuta
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  9. Zhen Chen
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  10. Hitoshi Chiba
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  11. Shu-Ping Hui
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Correspondence to Hitoshi Chiba or Shu-Ping Hui.

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Okabe, H., Shimizu, C., Yamamoto, M. et al. Determination of Serum 25-Hydroxyvitamin D3 by LC/MS/MS and Its Monthly Variation in Sapporo Indoor Workers. ANAL. SCI. 34, 1043–1047 (2018). https://doi.org/10.2116/analsci.18P193

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  • DOI: https://doi.org/10.2116/analsci.18P193

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